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Polyester vascular prostheses coated with a cyclodextrin polymer and activated with antibiotics: cytotoxicity and microbiological evaluation


, : Polyester vascular prostheses coated with a cyclodextrin polymer and activated with antibiotics: cytotoxicity and microbiological evaluation. Acta Biomaterialia 4(6): 1725-1733

Polyester (PET) vascular grafts are used to replace or bypass damaged arteries. To minimize the risk of infection during and after surgical interventions, a PET vascular prosthesis (Polythese) was functionalized with cyclodextrin polymers (PolyCDs) in order to obtain the controlled release of antibiotics (ABs: ciprofloxacin, vancomcyin and rifampicin). An epithelial cell line (L132) was used to determine the viability of the antibiotics, and human pulmonary microvascular endothelial cells (HPMEC) were used for cell proliferation by cell counting and cell vitality with Alamar Blue fluorescent dye. Staphylococcus aureus, Escherichia coli and Enteroccocus sp. were used to determine the antimicrobial activity of AB-loaded virgin and PolyCD-grafted Polythese by the minimum inhibitory concentration method. The spectrophotometric titration results first showed that a larger amount of ABs was sorbed onto PolyCD-coated Polythese compared to virgin Polythese (26.7 vs. 35.3 mg g(-1), 51.1 vs. 72.4 mg g(-1) and 4.1 vs. 21.0 mg g(-1), respectively, for rifampicin, vancomycin and ciprofloxacin). These results were further confirmed by a microbiological test, which showed AB-loaded PolyCD-coated Polythese displayed better antimicrobial activity. The viability test revealed the toxicity of rifampicin (22 mg l(-1)) and ciprofloxacin (35 mg l(-1)), and the absence of toxicity of vancomycin. These tests allow us to further explain the lower vitality and proliferation of HPMEC on the AB-loaded PolyCD-coated Polythese, which was due not to the functionalization process of prostheses but to the cytotoxicity of certain ABs themselves. Moreover, such a property could be exploited to tackle intracellular bacteria, such as in tuberculosis and other diseases, and will not compromise further in vivo applications of our functionalized vascular prostheses.

US$19.90

PMID: 18676187

DOI: 10.1016/j.actbio.2008.07.001


Other references

Breuning, S., 1980: Nouvelles especes de Lamiinae d'Asie orientale (Col. Cerambycidae). Bulletin de la Societe Entomologique de Mulhouse, (oct.-dec.): 49-50 .

Viana, Iés.G.; Bode, A., 2013: Stable nitrogen isotopes in coastal macroalgae: geographic and anthropogenic variability. Growing human population adds to the natural nitrogen loads to coastal waters. Both anthropogenic and natural nitrogen is readily incorporated in new biomass, and these different nitrogen sources may be traced by the measurement of the ratio of st...

Varma R.A.aykumar; Ramachandran K.K., 2001: Ground water recharge studies on Kavaratti Island, Union Territory of Lakshadweep, India. Cases of expanded extraction from the fragile fresh water lenses and resultant salinisation were reported from many Islands of Lakshadweep where pumping activity has been on the increase, especially, during the last two decades. This necessitated...

Dalziel Ian W.D., 1992: Pre-Mesozoic plate tectonics; new geologic ideas await paleomagnetic testing. Eos, Transactions, American Geophysical Union 73(14, special supplement): 93

Vorshilova, L.A.; Bylinkina, E.S.; Gorskaia, S.V., 1971: Study of the effect of aeration and agitation conditions on the cultivation of a streptomycin-producing microorganism. Antibiotiki 16(7): 613-615

Sharma, O.D., 1953: Treatment of 10 cases of pulmonary tuberculosis in Africans with isoniazid. East African Medical Journal 30(9): 385-388

Marz, P.; Ozbek, S.; Fischer, M., 2002: Differential response of neuronal cells to a fusion of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor. European Journal of Biochemistry 269(12): 23-31

Oliver, W.R.B., 1924: Strap-toothed Whale stranded at Kaitawa Point, entrance to Porirua Harbour pp. 187-188 1 fig. Stranded Blackfish at Marsden Point pp. 188-189 photo. New Zealand Journal of Science and Technology, 7

Vachharajani, T.J.; Zaman, F.; Latif, S.; Penn, R.; Abreo, K.D., 2005: Curvularia geniculata fungal peritonitis: a case report with review of literature. Peritonitis is the "Achilles heel" of patients oil peritonea] dialysis. Fungal peritonitis though not that common is associated with increased morbidity and mortality. We report the first case in tile literature of a patient who develope...

Dans, P.E., 1992: Medical students and abortion: reconciling personal beliefs and professional roles at one medical school. A survey was used from 1983 through 1990 in a required first-year course, Ethics and Medical Care, at The Johns Hopkins University School of Medicine, to explore where students drew the line about moral issues. Starting in 1988, a similar question...