+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer

, : Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer. Biological Chemistry 394(7): 889-900

Maackia amurensis agglutinin is a NeuNAcα (2-3) Galβ (1-4) GlcNAc/Glc-specific lectin, which was shown to have diagnostic potential in cancers of different origin. In a previous report, we demonstrated that GM3 specific IgG from bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients interacted with ∼66kDa membrane glycoprotein band of NSCLC cell lines, which was also recognised by this lectin. This observation prompted us to assess the potential of Maackia amurensis agglutinin in NSCLC. Accordingly, we examined the reactivity of this lectin with NSCLC cell lines as well as the tissue biopsies and cells obtained from fine needle aspirations of NSCLC patients. Maackia amurensis agglutinin showed strong reactivity, specifically with cells and biopsy samples of NSCLC origin. Furthermore, this lectin was found to induce apoptosis in NSCLC cells. The mechanism of this lectin-induced apoptosis involved downregulation of Bcl-XL, upregulation of Bax, release of cytochrome c and activation of procaspase-3. Collectively our results have suggested that Maackia amurensis agglutinin may have the potential to serve as a unique probe for detection of NSCLC and also as a specific apoptosis-inducing agent in NSCLC cells.


PMID: 23612596

DOI: 10.1515/hsz-2012-0279

Other references

McDougall, J.J.; Larson, S.E.M., 2006: Nociceptin/orphanin FQ evokes knee joint pain in rats via a mast cell independent mechanism. Nociceptin/orphanin FQ (N/OFQ) is an opioid-like neuropeptide that has been shown to cause peripheral sensitization of knee joint afferents; however, the effect of the peptide on joint pain behaviour is unknown. In addition to having a direct effe...

Baker, M.A.; Huang, S.S.; Letourneau, A.R.; Kaganov, R.E.; Peeples, J.R.; Drees, M.; Platt, R.; Yokoe, D.S., 2016: Lack of Comprehensive Outbreak Detection in Hospitals. Timely identification of outbreaks of hospital-associated infections is needed to implement control measures and minimize impact. Survey results from 33 hospitals indicated that most hospitals lacked a formal cluster definition and all targeted a...

Salim, R.; Cooksey, B.G., 1980: Kinetics of the adsorption of lead on river-muc. The organic matter fraction of river mud has a higher capacity for Pb adsorption than the inorganic fraction. Maximum adsorption occurs at pH 6.5. The rate of adsorption increases with mud concentration, and is proportional to the concentration of...

Mittal, S.R., 2012: Idiopathic dilatation of inferior vena cava. A case of isolated dilation of inferior vena cava with diminished inspiratory collapse is reported. There was no other abnormality. Diameter and collapsibility of IVC should be interpreted in collaboration with other clinical and echocardiographic...

Feinberg, S.S.; Opler, L.A., 1993: Pimozide-induced depression?. Journal of Clinical Psychopharmacology 13(1): 71-72

Stephens N., 1975: Climatic fluctuations of the ice age. Palaeogeography, Palaeoclimatology, Palaeoecology 18(1): 66-67

Kalenov G.S., 1985: The concept ecological niches of the desert plants based on space photography. Problemy Osvoeniya Pustyn' (6): 3-13

Besra G.S.; Minnikin D.E.; Rigouts L.; Portaels F.; Ridell M., 1990: A characteristic phenolic glycolipid antigen from mycobacterium haemophilum. Letters in Applied Microbiology 11(4): 202-204

Qiu, X.; Erickson, L., 1994: A simple and effective method for isolating RNA from alfalfa pollen. A method is described for isolating RNA from lucerne (Medicago sativa) pollen based on the combination of a simple homogenization device fashioned from a modified glass syringe and buffer. Adequate RNA was isolated from pollen extract after 30 sec...

Appeltant, R.; Somfai, Tás.; Maes, D.;, A.; Kikuchi, K., 2016: Porcine oocyte maturation in vitro: role of cAMP and oocyte-secreted factors - A practical approach. NlmCategory="UNASSIGNED">Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop...