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Potential usefulness of baculovirus-mediated sodium-iodide symporter reporter gene as non-invasively gene therapy monitoring in liver cancer cells: an in vitro evaluation


, : Potential usefulness of baculovirus-mediated sodium-iodide symporter reporter gene as non-invasively gene therapy monitoring in liver cancer cells: an in vitro evaluation. Technology in Cancer Research & Treatment 13(2): 139-148

Primary liver cancer has one of the highest mortality rates of all cancers, and the main current treatments have a poor prognosis. This study aims to examine the efficiency of baculovirus vectors for transducing target gene into liver cancer cells and to evaluate the feasibility of using baculovirus vectors to deliver the sodium-iodide symporter (NIS) gene as a reporter gene through co-vector administration approach to monitor the expression of the target therapeutic gene in liver cancer gene therapy. We constructed (green fluorescent protein) GFP- and NIS-expressing baculovirus vectors (Bac-GFP and Bac-NIS), and measured the baculovirus transduction efficiency in HepG2 cells and other tumor cells (A549, SW1116 and 8505C), and it showed that the transduction efficiency and target gene expression level rose with increasing viral multiplicity of infection (MOI) in HepG2 cells, and HepG2 cells had a significantly higher transduction efficiency (60.8% at MOI = 200) than other tumor cells. Moreover, the baculovirus transduction was not cytotoxic to HepG2 cells at a higher MOI (MOI 5 400). We also performed dynamic iodide uptake trials, and found that Bac-NIS-transduced HepG2 cells exhibited efficient iodide uptake which could be inhibited by sodium perchlorate (NaClO₄). And we measured the correlation of fluorescent intensities and 125I uptake amount in HepG2 cells after co-vector administration with Bac-NIS and Bac-GFP at different MOIs, and found a high correlation coefficient (r(2) = 0.8447), which provides a good basis for successfully evaluating the feasibility of baculovirus-mediated NIS reporter gene monitoring target gene expression in liver cancer therapy. Therefore, this study indicates that baculovirus vector is a potential vehicle for delivering therapeutic genes in studying liver cancer cells. And it is feasible to use a baculovirus vector to deliver NIS gene as a reporter gene to monitor the expression of target genes. It therefore provides an effective approach and a good basis for future baculovirus-mediated therapeutic gene delivering or therapeutic gene expression monitoring in liver cancer cells studies.

US$19.90

PMID: 23919394

DOI: 10.7785/tcrt.2012.500368


Other references

Zimmer, B.; Brune, I.; Kohler, B.; Wegener, K.; Schönleben, K.; Riemann, J.F., 1994: Mucinous cystadenomas and cystadenocarcinomas of the pancreas--the pancreaticoscopy as a new device for endoscopic and histological diagnosis. Recent advances in diagnostic imaging procedures offer the opportunity for detection of rare cystic neoplasms of the pancreas. Cystadenomas of the pancreas have been reported to represent 10% of cystic pancreatic lesions. Serous microcystic cystad...

Csekeő, A., 2015: In Process Citation. The significance of surgery in elderly patients with lung cancer is debated. The age is a predisposing risk factor for developing cancer thereby the possibilities of surgical procedure is increasing in the elderly patients. To clarify the benefit...

Lauterbach, F.O., 1975: The intestinal two-stage-transfer of sugars and its inhibition by phlorizin. Naunyn-Schmiedeberg's Archives of Pharmacology 287 Suppl: R69-R69

Gritti, I.; Mariotti, M.; Mancia, M., 1998: GABAaergic and cholinergic basal forebrain and preoptic-anterior hypothalamic projections to the mediodorsal nucleus of the thalamus in the cat. The present study examined projections of GABAergic and cholinergic neurons from the basal forebrain and preoptic-anterior hypothalamus to the "intermediate" part of the mediodorsal nucleus of the thalamus. Retrograde transport from this...

Carbon, J.; Ratzkin, B.; Clarke, L.; Richardson, D., 1977: The expression of cloned eukaryotic DNA in prokaryotes. Brookhaven Symposia in Biology: 277-296

Dijkhof, J.; Poort, S.R.; Poort, C., 1977: Effect of feeding soybean flour containing diets on the protein synthetic pattern of the rat pancreas. The effect was studied of a diet with raw (RSD) or heated (HSD) soya bean flour on synthesis in vitro of pancreatic secretory proteins. After male Wistar rats 2-12 weeks old had been fed on either diet for 5 days, they were killed; one half of the...

Erlich M., 1982: Stochastic models of prediction of linear flows. Archiwum Hydrotechniki 29(1-2): 3-31

Watkins, L.M.; Rodriguez, R.; Schneider, D.; Broderick, R.; Cruz, M.; Chambers, R.; Ruckman, E.; Cody, M.; Mrachko, G.T., 2003: Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase. 2-(2'-Hydroxyphenyl)benzenesulfinate desulfinase (HPBS desulfinase) catalyzes the cleavage of the carbon-sulfur bond of 2-(2'-hydroxyphenyl)benzenesulfinate (HPBS) to form hydroxybiphenyl and sulfite. This is the final step in the desulf...

Diaz, R.Jose.; Ali, S.; Qadir, M.Gull.; De La Fuente, M.I.; Ivan, M.E.; Komotar, R.J., 2017: The role of bevacizumab in the treatment of glioblastoma. Bevacizumab has been used in patients with GBM as a salvage therapy since its approval in the United States for recurrent GBM in 2009. In order to review the therapeutic effect of bevacizumab in the primary and recurrent clinical setting we have p...

Harrison-Lowe, N.J.; Olsen, L.J., 2008: Autophagy protein 6 (ATG6) is required for pollen germination in Arabidopsis thaliana. Autophagy is an intracellular recycling pathway that extends the life of an organism in hostile growth conditions. Yeast autophagy protein 6 (Atg6Nps30) is required for formation of autophagosomes during starvation. Here we show that the Arabidop...