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Prep1 (pKnox1) regulates mouse embryonic HSC cycling and self-renewal affecting the Stat1-Sca1 IFN-dependent pathway

, : Prep1 (pKnox1) regulates mouse embryonic HSC cycling and self-renewal affecting the Stat1-Sca1 IFN-dependent pathway. Plos One 9(9): E107916-E107916

A hypomorphic Prep1 mutation results in embryonic lethality at late gestation with a pleiotropic embryonic phenotype that includes defects in all hematopoietic lineages. Reduced functionality of the hematopoietic stem cells (HSCs) compartment might be responsible for the hematopoietic phenotype observed at mid-gestation. In this paper we demonstrate that Prep1 regulates the number of HSCs in fetal livers (FLs), their clonogenic potential and their ability to de novo generate the hematopoietic system in ablated hosts. Furthermore, we show that Prep1 controls the self-renewal ability of the FL HSC compartment as demonstrated by serial transplantation experiments. The premature exhaustion of Prep1 mutant HSCs correlates with the reduced quiescent stem cell pool thus suggesting that Prep1 regulates the self-renewal ability by controlling the quiescence/proliferation balance. Finally, we show that in FL HSCs Prep1 absence induces the interferon signaling pathway leading to premature cycling and exhaustion of fetal HSCs.


PMID: 25233378

DOI: 10.1371/journal.pone.0107916

Other references

Anonymous, 1992: 15th International Symposium on Column Liquid Chromatography. Part II. Basel, June 3-7, 1991. Journal of Chromatography 593(1-2): 1-378

Hottz, E.D.; Medeiros-de-Moraes, I.M.; Vieira-de-Abreu, A.; de Assis, E.F.; Vals-de-Souza, Rério.; Castro-Faria-Neto, H.C.; Weyrich, A.S.; Zimmerman, G.A.; Bozza, F.A.; Bozza, Pícia.T., 2014: Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue. Dengue is the most prevalent human arbovirus disease in the world. Dengue infection has a large spectrum of clinical manifestations, from self-limited febrile illness to severe syndromes accompanied by bleeding and shock. Thrombocytopenia and vasc...

Belardinelli, E.; Ursino, M.; Fabbri, G.; Cevese, A.; Schena, F., 1991: Pressure changes induced by whole body acceleration shocks. In the present paper pressure changes induced by sudden body acceleration are studied "in vivo" on the dog and compared to the results obtainable with a recently developed mathematical model. A dog was fixed to a moveable table, which wa...

Weisswange, A., 1996: Dietary measures and lipid lowering. Roskamm, H [Author], Reindell, H [Author] [Heart diseases: Pathophysiology, diagnosis, therapy, 4th edition] Herzkrankheiten: Pathophysiologie, Diagnostik, Therapie, 4 Auflage : 1550-1574

Inoue, K.; Wen, R.; Rehg, J.E.; Adachi, M.; Cleveland, J.L.; Roussel, M.F.; Sherr, C.J., 2000: Disruption of the ARF transcriptional activator DMP1 facilitates cell immortalization, Ras transformation, and tumorigenesis. The DMP1 transcription factor induces the ARF tumor suppressor gene in mouse fibroblasts, leading to cell cycle arrest in a p53-dependent manner. We disrupted sequences encoding the DNA-binding domain of DMP1 in mouse embryonic stem cells and deri...

Boyd, H.B.; Griffin, L.L., 1949: Classification and treatment of trochanteric fractures. Archives of Surgery 58(6): 853-866

Lundquist Gary M.; Howell B.F.J., 1969: Frequency-band and integrated magnitude using long-period seismograms. Eos, Transactions, American Geophysical Union 50(4): 238

Charrin Victor, 1956: Les kaolins metropolitains. Briefly describes primary kaolin deposits associated with granitic and crystalline schist complexes of France, and secondary deposits which occur mainly in Eocene terrain.

Ganapati, P.N.; Rao, D.V.S.bba, 1957: On upwelling and productivity of the waters off Lawson s Bay, Waltair. Current Sci 26(11): 347-348

Wolffram, S.; Eggenberger, E.; Scharrer, E., 1986: Kinetics of D-glucose and L-leucine transport into sheep and pig intestinal brush border membrane vesicles. The kinetic parameters (Vmax, Kt) of Na+-dependent D-glucose transport into brush border membrane vesicles (BBMV) from sheep and pig jejunum were determined. Due to the fermentation of ingested carbohydrates in the rumen the small intestine of rum...