+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prevalence and characterization of quinolone resistance mechanisms in commensal Escherichia coli isolated from slaughter animals in Poland, 2009-2012

, : Prevalence and characterization of quinolone resistance mechanisms in commensal Escherichia coli isolated from slaughter animals in Poland, 2009-2012. Microbial Drug Resistance 20(6): 544-549

The background of quinolone resistance was characterized in ciprofloxacin-resistant commensal Escherichia coli selected out of 3,551 isolates from slaughtered animals in Poland between 2009 and 2012. Plasmid-mediated determinants were suspected in 6.2% of the study group, ranging from 1.1% in cattle to 9.7% in turkeys. Polymerase chain reaction and sequencing identified up to four quinolone resistance-determining substitutions in gyrA (Ser83, Asp87) and parC (Ala56, Ser80). Plasmid-mediated mechanisms were identified as qnrS1 (or qnrS3, n=70, including six isolates with chromosomal mutations), qnrB19 (or qnrB10, n=19), and qnrB17 (n=1). All tested isolates were negative for qnrA, qnrC, qnrD, qepA, and aac(6')-Ib-cr. Still, there were several E. coli suspected for both plasmid- and chromosome-mediated resistance with unrevealed genetic background of the phenomenon. Since all tested isolates showed diverse XbaI-PFGE profiles, chromosome-encoded quinolone resistance does not result from the spread of a single resistant clone, however, it is rather due to antimicrobial pressure leading to the selection of random gyr and par mutants. It also favors the selection and spread of plasmids carrying predominant qnr genes, since the same determinants were found in Salmonella, isolated from similar sources. The identification of carrier plasmids and mitigation of their spread might be essential for sustainable quinolone usage in animal husbandry and efficient protection of human health.


PMID: 25051094

DOI: 10.1089/mdr.2014.0061

Other references

Okolov, F.S.; Imanbaev, S.I., 1966: Use of ethyl alcohol as an antifreeze for the study in winter of the natural ultraviolet radiation level using the oxalate method. Gig Sanit 31(10): 86-87

Aziz, A.; Bajwa, I.Ullah., 2007: Minimizing human health effects of urban air pollution through quantification and control of motor vehicular carbon monoxide (CO) in Lahore. Impact of urban air pollution has variety of focuses such as urban ecology, human health, economy, etc. But human health is always given priority. Air pollution is threat to the lives of people living in big cities of Pakistan. In Lahore only ther...

Foeken, A.W.J. van; Stadhouders, J.; Witsenburg, W.W., 1978: The number of enterotoxigenic Staphylococcus aureus in 24-hour old Gouda cheese made under optimal manufacturing conditions and the amount of enterotoxin produced. In Gouda cheese made under optimum conditions, enterotoxin could always be detected when the Staph. aureus count exceeded 108/g; levels of enterotoxin ranged from 0.5 to 2.0 mu g/100 g cheese. Strains differed in the amount of enterotoxin produced...

Mombelli, A.; Geering, A.H., 1982: Esthetic effect of dental prosthesis. Schweizerische Monatsschrift für Zahnheilkunde 92(7): 586-596

Hoving, J.; Roosjen, H.N.; Brouwers, J.R., 1988: Adverse reactions to technetium-99m methylene diphosphonate. Journal of Nuclear Medicine 29(7): 1302-1303

Salathe, M.; Lipson, E.J.; Ivonnet, P.I.; Bookman, R.J., 1997: Muscarinic signaling in ciliated tracheal epithelial cells: dual effects on Ca2+ and ciliary beating. To examine cholinergic signal transduction pathways that modulate ciliary beat frequency (CBF), cultured ovine tracheal epithelial cells were imaged using a combination of phase-contrast (CBF) and fluorescence (Ca-2+) microscopy techniques. In sin...

Papousek, H., 1973: Group rearing in day care centers and mental health: potential advantages and risks. Research Publications - Association for Research in Nervous and Mental Disease 51: 398-411

Kozlowski, R.Z.; Ashford, M.L., 1992: Nucleotide-dependent activation of KATP channels by diazoxide in CRI-G1 insulin-secreting cells. 1. Patch-clamp recording techniques were used, to examine the effects of diazoxide on KATP currents in CRI-G1 insulin-secreting cells in the presence of non-hydrolysable nucleotides. 2. In the presence of non- or slowly-hydrolyzed ATP analogues, b...

De, H.N.; Bhuiya, E.H.; Debnath, J.C., 1965: Biochemical and nutritional studies on East Pakistan vegetables. 2. Distribution of protein and non-protein nitrogen in different parts of vegetables. For part 1 see Abst. 6082, Vol. 33. 2. The skin and flesh of fruity vegetables and the leaves and stems of leafy vegetables grown in East Pakistan were analysed for total N and non-protein N. The skin and leaves contained more protein than the fle...

Zhang, R.; Lee, B.; Stafford, C.M.; Douglas, J.F.; Dobrynin, A.V.; Bockstaller, M.R.; Karim, A., 2017: Entropy-driven segregation of polymer-grafted nanoparticles under confinement. The modification of nanoparticles with polymer ligands has emerged as a versatile approach to control the interactions and organization of nanoparticles in polymer nanocomposite materials. Besides their technological significance, polymer-grafted...