+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prevention of spontaneous abortion in the CBA x DBA/2 mouse model by intravaginal TGF-beta and local recruitment of CD4+8+ FOXP3+ cells

, : Prevention of spontaneous abortion in the CBA x DBA/2 mouse model by intravaginal TGF-beta and local recruitment of CD4+8+ FOXP3+ cells. American Journal of Reproductive Immunology 59(6): 525-534

ProblemActivation of latent transforming growth factor (TGF)-beta in seminal plasma has been suggested by Robertson et al. to promote maternal tolerance to paternal antigens. A possible consequence reported by Tremellen et al. is increased pregnancy rates in women undergoing IVF. A decreased spontaneous abortion rate has also been postulated. Seminal plasma contains many factors besides TGF-beta, and a critical test of the hypothesis was required. The purpose of the present study was to directly test the effect of pure TGF-beta.Method of studyPharmaceutical grade bioactive TGF-beta 3 with a bovine serum albumin (BSA) carrier 0.1-1% in phosphate-buffered saline (PBS) was given into the vaginal tract of CBA/J female mice at the time of mating with DBA/2 males. One microgram Salmonella enteritidis lipopolysaccharide was given intraperitoneally to augment occult losses and spontaneous resorptions assessed on day 13.5 of pregnancy. The effect of TGF-beta 3 on recruitment of lymphomyeloid cells to the vaginal wall and vaginal lumen of unmated mice in estrus was assessed using immunohistochemistry and flow cytometry.ResultsTwo nanogram of intravaginal TGF-beta 3 in 0.1% BSA-PBS or 20 ng in 1% BSA-PBS reduced abortion rates. Protection was comparable to that achieved by immunization with BALB/c spleen cells. Fraction V BSA, a binder of TGF-beta s, had some activity, and could reduce availability of added TGF-beta 3. CD11c dendritic cells, CD3(+) T cells, and CD25(+) cells were recruited to the vaginal wall by 48 hr after TGF-beta 3 treatment, and cellularity of vaginal exudates increased. Foxp3(+) cells were present in increased numbers, and appeared to be CD8(+) and CD4(+) 8(+). Semen, but not TGF-beta 3, stimulated a physiological polymorphonuclear leukocyte exudate.ConclusionIntravaginal bioactive TGF-beta 3 can enhance success of pregnancy in vivo in an established model of abortion. The result could be explained by the independent ability of TGF-beta to promote a regulatory T-cell response.


PMID: 18410469

DOI: 10.1111/j.1600-0897.2008.00582.x

Other references

Hund, T.; Ascher, B.; Rzany, B., 2006: Reproducibility of two four-point clinical severity scores for lateral canthal lines (crow's feet). Several clinical scoring systems have been used to evaluate the efficacy of botulinum toxin A in the treatment of hyperkinetic wrinkles. So far very few have been investigated for their reproducibility. The aim of this study was to investigate the...

Hungr Oldrich, 1997: Mechanisms of rockfalls and rockslides. Annual Meeting Association of Engineering Geologists: ages 113. 1997.

Dennis, D.L.; Fletcher, W.S., 1966: Toxicity of sodium thiosulfate (NSC-45624), a nitrogen mustard antagonist, in the dog. Cancer ChemoTherapy Reports 50(5): 255-257

Nakamura, H.; Koizumi, H.; Kimura, H.; Marushima, H.; Saji, H.; Takagi, M., 2016: Epidermal growth factor receptor mutations in adenocarcinoma in situ and minimally invasive adenocarcinoma detected using mutation-specific monoclonal antibodies. Epidermal growth factor receptor (EGFR) mutation rates in adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA) were studied using both DNA analysis and mutation-specific immunohistochemistry. The peptide nucleic acid-locked nuc...

Su, W-Chao.; Lin, Y-Feng.; Yu, X-Ping.; Wang, Y-Xia.; Lin, X-Dong.; Su, Q-Zhen.; Shen, D-Yan.; Chen, Q-Xi., 2017: Mitochondria-Associated Apoptosis in Human Melanoma Cells Induced by Cardanol Monoene from Cashew Nut Shell Liquid. Cardanol monoene (CM) is the major phenolic component extracted from cashew nut shell liquid (CNSL), which has been relevant to wide range of biological effects. In this study, we found that CM could inhibit the M14 human melanoma cells proliferat...

Yoshidome, K.; Shibata, M.-Aki; Couldrey, C.; Korach, K., S.; Green, J., E., 2000: Estrogen promotes mammary tumor development in C3(1)/SV40 large T-antigen transgenic mice: Paradoxical loss of estrogen receptor alpha expression during tumor progression. Although several lines of epidemiological evidence suggest that estrogen exposure influences the incidence of breast cancer development, the mechanisms by which estrogen may stimulate the formation of breast cancer remain poorly understood. We hav...

Patoine, B., 2009: NerveCenter: Stem cells surge forward to clinical trials, but fears remain. Annals of Neurology 65(5): A9-A11

Kiorboe, Thomas, 2007: Mate finding, mating, and population dynamics in a planktonic copepod Oithona davisae: There are too few males. Three- dimensional ( 3D) video observations of free- swimming copepods revealed the mechanisms of mate finding in the cyclopoid copepod Oithona davisae ( cephalothorax length 0.33 mm). The females are ambush feeders and spend almost all of their...

Singh, R.; Tandon, I.; Deo, S., 2008: Fraser syndrome: recurrence in a family. Fraser syndrome is a rare, autosomal recessive condition with classical features of cryptophthalmos, syndactyly, ambiguous genitalia, genitourinary mal-formations and mental retardation. We report a family with affected child where the pregnant wo...

Okumus, I., 2003: Breeding stock management - III: hatchery production. Recurrent problems in the culture of marine fishes such as sea trout (Salmo trutta), gilt head bream (Pagrus aurata), sea bass (Dicentrarchus labrax) and turbot (Scophthalmus maximus) include the timing of ovulation and/or egg production, uncertai...