+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Probing the effect of temperature on the backbone dynamics of the human alpha-lactalbumin molten globule

, : Probing the effect of temperature on the backbone dynamics of the human alpha-lactalbumin molten globule. Journal of the American Chemical Society 130(46): 15318-15326

Molten globules are compact, partially folded proteins postulated to be general intermediates in protein folding. Human alpha-lactalbumin (alpha-LA) is a two-domain Ca(2+)-binding protein that partially unfolds at low pH to form a molten globule. NMR spectra of molten globules are characterized by broadened resonances due to conformational fluctuations on microsecond to millisecond time scales. These species are often studied at high temperature where NMR resonances are observed to sharpen. The effect of higher temperatures on fast time-scale backbone dynamics of molten globules has not been investigated previously. Here, 1D (15)N direct-detection and 2D indirect-detection (1)H-(15)N heteronuclear NOE experiments have been used to probe fast time-scale dynamics at low and high temperatures for three disulfide-bond variants of human alpha-LA that form molten globules. Disulfide bonds are found to have a significant effect on backbone dynamics within the beta-domain of the molten globule; within the alpha-domain, dynamics are not significantly influenced by these bonds. At 20 degrees C, backbone mobility is significantly decreased in both domains of the molten globule compared to the mobility at 40-50 degrees C. Heteronuclear NOE values determined at 20 degrees C for the alpha-domain are closely similar to those observed for native alpha-LA, indicating that the alpha-LA molten globule has even more native-like character than suggested by studies conducted at higher temperature. Our results highlight the importance of considering the temperature dependence of the molten globule ensemble when making comparisons between experimental data obtained under different conditions.


PMID: 18939797

DOI: 10.1021/ja802967k

Other references

Böttcher, D.; Nissen, P.; Harmjanz, D., 1972: Oral administration of N-propyl-ajmaline bitartrate in heart rhythm disorders. Medizinische Klinik 67(24): 855-859

Olmstead, A.; Rhode, P., 1995: Beyond the threshold: an analysis of the characteristics and behavior of early reaper adopters. Journal of economic history 55(1): 27-57

Filewood, W., 1984: 7 New Zealand, New Guinea Lord Howe Is 72 The Torres connection zoogeography of New Guinea. Unknown

Gavazzi, G.; Sandri, M., 1978: Further analysis of duplication strands with a duplicate segment in a displaced position on the long arm of chromosome 10. Previous studies have shown that the duplications Dp10-1, Dp10-2, Dp10-3 and Dp10-4 lie on the long arm of chromosome 10 and that in the case of Dp10-1 the duplication is terminal. Addition linkage analyses indicated that Dp10-1 is probably distal...

Cousins, A.B.; Badger, M.R.; von Caemmerer, S., 2008: C4 photosynthetic isotope exchange in NAD-ME- and NADP-ME-type grasses. Monitoring photosynthetic isotope exchange is an important tool for predicting the influence of plant communities on the global carbon cycle in response to climate change. C(4) grasses play an important role in the global carbon cycle, but their c...

Heidarifard, M.H.; Seraj, M.; Shayesteh, M.; Ghalavand, H.; A.K.alifa, S. bin Salman, 2008: Geothermal gradient study of Asmari Formation in Dezful Embayment, Zagros, Iran. This study was focused on determining the geothermal gradient of the Oligocene-Miocene Asmari Reservoir in the Dezful Embayment, Iran. It involved preparing an isothemal map of the reservoir, recognition of a temperature anomaly zone and the study...

Anonymous, 1976: Emphasize the positive. Tic 35(6): 13-13

Nygren, J.M.; Andersson, L.; Jacobsen, S.E.rik, 2002: Actively Proliferating Adult Hematopoietic Stem Cells Reside Predominantly in G1 of the Cell Cycle but Are Not Compromised in Engrafting Ability When Transiting S/G2/M. The ability of adult hematopoietic stem cells (HSC) to engraft following transplantation is thought to be restricted to HSC residing in G0 or G1 of the cell cycle, whereas HSC transiting S/G2/M have been hypothesized to be severely compromised in...

Sutiagin, V.I., 1993: A case of the successful late surgical treatment of double posttraumatic stenosis of the right primary and intermediate bronchi. Grudnaia i Serdechno-Sosudistaia Khirurgiia: 60-62

Kwon, O.K.; Bae, H.D.; Shin, H.T., 1991: Effects of urea-starch product (SKKTU-II) supplementation on the performance of lactating dairy cows. The experiment was conducted to evaluate the effect of the urea-starch product (SKKTU-II) as a substitute for soyabean meal for lactating dairy cows on their performance, measuring the daily feed intake, milk yield, milk composition, blood ammonia...