+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prosaposin expression in the regenerated muscles of mdx and cardiotoxin-treated mice

, : Prosaposin expression in the regenerated muscles of mdx and cardiotoxin-treated mice. Histology and Histopathology 28(7): 875-892

The trophic factor prosaposin (PS) is strongly expressed in skeletal muscle, and reportedly, a PS-derived peptide attenuates loss of muscle mass after nerve injury in vivo and increases myoblast fusion into myotubes in vitro. However, few studies have focused on the role of PS during muscle regeneration. We examined the expression of PS in the skeletal muscles in normal, mdx, and cardiotoxin (CTX)-treated mice using immunofluorescence staining, Western blotting, and in situ hybridisation. Immunofluorescence showed intense PS immunoreactivity in the peripheral cytoplasm of uninjured myofibres of normal mice and regenerated myofibres of 8 weeks post-CTX-injection mice. In early stage CTX-treated mice (14 days and earlier), intense PS immunoreactivity was also detected in the immune cells that infiltrated damaged muscle, but it was weak for regenerating myofibres. Western blot confirmed these findings. In contrast, PS was continuously low in mdx mice in both immunofluorescence and Western blotting. In situ hybridisation confirmed the decrease of PS mRNA in regenerated myofibres and revealed the main form of PS mRNA as Pro+0 without a 9-base insertion both in normal and mdx mice. The embryonic myosin (MYH3) was clearly localized in the newly regenerated myofibres at 3, 7, and 14 days of post-CTX-injection and mdx mice, but was lower in the late stage of regenerated myofibres (28 and 56 days post-CTX injection). The inverse distribution of MYH3 and PS indicates that the PS expression is closely related to the differentiation of regenerated myofibres. Investigation of the mitogen-activated protein (MAP) kinase signal pathway showed the inversely synchronous correlation of phosphorylated ERK1/2 with myofibre PS and the synchronous correlation of phosphorylated p-38 with myofibre PS. These data suggest that PS is involved in the regulation of muscle differentiation of regenerated fibres.


PMID: 23325523

DOI: 10.14670/HH-28.875

Other references

Melamed, R.; Boas, R.C.V.llas, 2000: Application of physico-chemical amendments for the counteraction of mercury pollution. In order to counteract mercury pollution, due to gold recovery practices, in situ strategies are required. In this study, some physico-chemical additions were tested, in different environmental compartments, to verify their potential to mitigate m...

Genuis, S.J., 2008: Toxic causes of mental illness are overlooked. While proper brain function requires the complex interaction of chemicals perpetually occupied in purposeful biochemistry, it is well established that certain toxic substances have the potential to disrupt normal brain physiology and to impair neu...

Petukhov, N.A.; Petukhov, V.N., 2002: The effect of the teat rubber of a milking unit on the milk-release reflex. An analysis is made of the action of the teat rubber during the milking process, milk release being stimulated mainly by vibration (9-11 Hertz). Formulae are derived for expressing the relationships of the various parameters involved. A device (de...

Hamidinia, A.; Beck, A.D.; Van Beek, A.L., 1979: Modified technique for microsurgical vasovasostomy. 25 Sprague-Daveley rats had bilateral division of the vas deferens with immediate microsurgical anastomosis. The 2-layer microsurgical vasovasostomy as described in detail tin this report permits continuous imspection of the lumen. To determine th...; M.W.i; Zhang Changqing; Chang Xiaoxiao; Liu Yongzhi, 1993: Experimental research on deformation in model of an artificially frozen wall. International Conference on Permafrost, Proceedings 6, Vol

Kirkpatrick, B.W.; Dinkel, C.A.; Rutledge, J.J.; Hauser, E.R., 1985: Prediction equations of beef cow efficiency. Data from 107 beef (Hereford), dairy (Holstein-Friesian), and beef X dairy crossbred cows and their calves born in 3 parities were used to develop prediction equations of cow efficiency. Efficiency was defined as the ratio of calf weaning weight t...

Pollini H., 1967: Thrombotic changes due to dys proteinemia in athero sclerotic and thrombo phlebitic patients. Sangre (saragossa): 363-374

Schreiber, U.V.daver, W., 1973: Photosynthetic energy transfer reversibly inhibited by hydrostatic pressure. Photochemistry and photobiology: 18 (3) 205-208

Valdimarsson, T.; Arnqvist, H.J.; Toss, G.; Jarnerot, G.; Nystrom, F.; Strom, M., 1999: Low circulating insulin-like growth factor I in coeliac disease and its relation to bone mineral density. Background: Patients with coeliac disease have low bone mineral density (BMD), but the underlying mechanisms are unclear. Our aim was to study circulating insulin-like growth factor I (IGF-I) and its possible relationship to BMD in adults with unt...

Carasi, P.; Racedo, S.María.; Jacquot, C.; Elie, A.Marie.; Serradell, MíÁngeles.; Urdaci, Mía.C., 2017: iEnterococcus durans EP1 a Promising Anti-inflammatory Probiotic Able to Stimulate sIgA and to Increase iFaecalibacterium prausnitzii Abundance. <i>Enterococcus</i> species, principally <i>Enterococcus faecium</i> are used as probiotics since a long time with preference in animal applications but safety considerations were updated and also new uses as probiotics can...