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Protection of apigenin against kainate-induced excitotoxicity by anti-oxidative effects


, : Protection of apigenin against kainate-induced excitotoxicity by anti-oxidative effects. Biological & Pharmaceutical Bulletin 35(9): 1440-1446

Apigenin (5,7,4'-trihydroxyflavone) is a principal ingredient of Cirsium japonicum. These experiments were performed to determine whether apigenin has neuroprotective effects against kainic acid (KA)-induced excitotoxicity in vitro and in vivo. Intraperitoneal (i.p.) administration of apigenin (25, 50 mg/kg) decreased the seizure scores induced by KA injection (40 mg/kg, i.p.) in mice. In addition, the convulsion onset time was significantly delayed by apigenin administration. Moreover, we found that apigenin blocked KA-induced seizure-form electroencephalogram (EEG) discharge activity in the brain cortex. In hippocampal cells, apigenin inhibited KA-induced excitotoxicity in a dose-dependent manner as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. To study the possible mechanisms underlying the in vitro neuroprotective effects of apigenin against KA-induced cytotoxicity, we also examined the effect of apigenin on intracellular reactive oxygen species (ROS) elevations in cultured hippocampal neurons and found that apigenin treatment dose-dependently inhibited intracellular ROS elevation. The remarkable reduction of glutathione (GSH) levels induced by KA in hippocampal tissues was reversed by apigenin in a dose-dependent manner. In addition, similar results were obtained after pretreatment with free radical scavengers such as trolox and dimethylthiourea (DMTU). Finally, after confirming the protective effect of apigenin in hippocampal CA3 region, we found apigenin is an active compound in KA-induced neuroprotection. These results collectively indicate that apigenin alleviates KA-induced excitotoxicity by quenching ROS as well as inhibiting GSH depletion in hippocampal neurons.

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PMID: 22975493


Other references

Rubin, B.A., 1993: Clinical picture and epidemiology of adenovirus infections (a review). Adenoviruses produce a variety of serious diseases in people of all ages. The mode of transmission of adenovirus infections includes respiratory, fomite, droplet, venereal, and faecal-oral routes. They have been shown to spread with ease in AIDS p...

Wang, S.; Zhang, J., 1995: AgNOR, DNA ploidy and prognosis in colorectal cancer. A follow-up study with special reference to nucleolarorganizer region-associated protein (AgNOR) and DNA ploidy was conducted in 83 consecutive cases of colorectal cancer patients who underwent radical surgery at the people's Hospital, Beijin...

Speake, D.W.; Hill, E.P.; Carter, V.E., 1975: Aspects of land management with regard to production of wood and wildlife in the southeastern United States. Discusses the habitat requirements of game species (white-tailed deer, bobwhite quail and wild turkey) in relation to the silvicultural management of Southern Pine stands and especially the practice of controlled burning. Data are presented for th...

Shi, Y.; Yao, J.; Xu, G.; Taber, L.A., 2015: Bending of the looping heart: differential growth revisited. In the early embryo, the primitive heart tube (HT) undergoes the morphogenetic process of c-looping as it bends and twists into a c-shaped tube. Despite intensive study for nearly a century, the physical forces that drive looping remain poorly und...

Campbell J.M.; Reusser F.; Caskey C.T., 1979: Specificity of lincomycin action on peptidyl transferase activity. The antibiotic lincomycin and 12 of its analogs were analyzed for their effects on 3 [Escherichia coli] peptidyl transferase reactions, peptide bond formation, esterification and hydrolysis of formylmethionyl-tRNA. Only lincomycin stimulated hydro...

Bengoechea-Alonso, M.T.; Punga, T.; Ericsson, J., 2005: Hyperphosphorylation regulates the activity of SREBP1 during mitosis. The sterol regulatory element-binding protein (SREBP) family of transcription factors controls the biosynthesis of cholesterol and other lipids, and lipid synthesis is critical for cell growth and proliferation. We were, therefore, interested in t...

Ossowska, K.; Wolfarth, S., 1995: Lack of a strong influence of neuroleptic decanoates on dopaminergic and GABAergic functions. Data concerning the incidence of extrapyramidal symptoms and the development of the supersensitivity to dopamine after administration of depot neuroleptics are controversial. The aim of the study was to examine the influence of depot neuroleptics...

Batlle, D.C.; Alpern, R.J., 1991: Regulation of intracellular pH. Introduction. Seminars in Nephrology 11(1): 1-2

Bekedam, D., 1973: Aminopeptidase activity in carious dentin. Chronicle 36(5): 129-129

Burton, R., 1969: The aggressive bonxie. Animals, 11: 402-405