+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Protective effects of alanyl-glutamine supplementation against nelfinavir-induced epithelial impairment in IEC-6 cells and in mouse intestinal mucosa

, : Protective effects of alanyl-glutamine supplementation against nelfinavir-induced epithelial impairment in IEC-6 cells and in mouse intestinal mucosa. Cancer Biology & Therapy 13(14): 1482-1490

Human Immunodeficiency Virus (HIV) protease inhibitors (PI) remain a crucial component of highly active therapy (HAART) and recently have been demonstrated to have potent antitumor effect on a wide variety of tumor cell lines. However, discontinuation of therapy is an important issue, which may be related to various side-effects, especially diarrhea. The aim of this study was to evaluate the effects of nelfinavir (NFV), an HIV PI, and of alanyl-glutamine (AQ) supplementation, on intestinal cell migration, proliferation, apoptosis and necrosis, using IEC-6 cells and on intestinal crypt depth, villus length, villus area, mitotic index and apoptosis in Swiss mice. Migration was evaluated at 12 and 24 h after injury using a wound healing assay. Cellular proliferation was measured indirectly at 24 and 48 h using tetrazolium salt WST-1. Apoptosis and necrosis were measured by flow cytometry using the Annexin V assay. Intestinal morphometry and mitotic index in vivo were assessed following a seven-day treatment with 100 mg/kg of NFV, given orally. In vivo proliferation and apoptosis were evaluated by intestinal crypt mitotic index and immunohistochemistry, respectively. In vitro, AQ supplementation enhanced IEC-6 cell migration and proliferation, following challenge with NFV. In vivo, AQ increased intestinal villus length, villus area, crypt depth and cell proliferation and cell migration, following treatment with NFV. AQ did not decrease cell death induced by NFV both in vivo and in vitro. AQ supplementation is potentially beneficial in preventing the effects of PIs, such as NFV, in the intestinal tract.


PMID: 22986234

DOI: 10.4161/cbt.22251

Other references

Laderach, D.; Wesa, A.; Galy, A., 2003: 4-1BB-ligand is regulated on human dendritic cells and induces the production of IL-12. Co-stimulation via 4-1BB and its ligand 4-1BB ligand (4-1BB-L) plays an important role in cytotoxic and pro-inflammatory immune responses. 4-1BB-L is generally described on activated antigen-presenting cells but there is limited information on its...

Bayer, C.Roth.; Eckstrand, K.L.; Knudson, G.; Koehler, J.; Leibowitz, S.; Tsai, P.; Feldman, J.L., 2017: Sexual Health Competencies for Undergraduate Medical Education in North America. The number of hours spent teaching sexual health content and skills in medical education continues to decrease despite the increase in sexual health issues faced by patients across the lifespan. In 2012 and 2014, experts across sexuality disciplin...

Sidhu, P.S.; Bhangu, S.S., 1993: Distribution of water soluble and exchangeable potassium in benchmark soils of Punjab. Journal of the Indian Society of Soil Science 41(4): 678-682

Kravchenko, A.I., 1963: Experimental Studies On Some Bicyclic Bis-(Beta-Chloroethyl) Amines. Voprosy Onkologii 9: 35-39

Higgins, S.T.; Heil, S.H.; Solomon, L.J.; Bernstein, I.M.; Lussier, J.Plebani.; Abel, R.L.; Lynch, M.Ellen.; Badger, G.J., 2005: A pilot study on voucher-based incentives to promote abstinence from cigarette smoking during pregnancy and postpartum. We report results from a pilot study examining the use of vouchers redeemable for retail items as incentives for smoking cessation during pregnancy and postpartum. Of 100 study-eligible women who were still smoking upon entering prenatal care, 58...

Frakking, F.N.J.; Brouwer, N.; Dolman, K.M.; van Woensel, J.B.M.; Caron, H.N.; Kuijpers, T.W.; van de Wetering, M.D., 2011: Mannose-binding lectin (MBL) as prognostic factor in paediatric oncology patients. Deficiency of mannose-binding lectin (MBL) has been suggested to influence duration of febrile neutropenia and prognosis in paediatric oncology patients. However, there is no consensus on the definition of MBL deficiency. In a cohort of children w...

Bobrinev, Vp, 1978: Method of sowing and field germination ability of seed. Lesnoe khoziaistvo: ) 66-67

Liberski, P.P.; Yanagihara, R.; Wells, G.A.; Gajdusek, D.C., 1993: Bovine spongiform encephalopathy in cattle mimics ultrastructurally experimental scrapie and Creutzfeldt-Jakob disease in rodents. We report a comparison of the ultrastructural pathology of bovine spongiform encephalopathy, experimental scrapie in hamsters and a panencephalopathic model of Creuzfeldt-Jakob disease (CJD) in mice. Vacuoles in dendrites, intramyelinic vacuoles (...

Tuggle, C.K.; Fuchs, J.A., 1986: Regulation of the operon encoding ribonucleotide reductase in Escherichia coli: evidence for both positive and negative control. The ribonucleotide reductase genes (nrd) are induced by thymine starvation. Deletion analysis of the sequences upstream of the cloned nrd genes was used to identify several regulatory regions. The start of transcription (nrdP) was mapped 110 bp up...

Papadimitriou-Olivgeris, M.; Drougka, E.; Fligou, F.; Kolonitsiou, F.; Liakopoulos, A.; Dodou, V.; Anastassiou, E.D.; Petinaki, E.; Marangos, M.; Filos, K.S.; Spiliopoulou, I., 2015: Risk factors for enterococcal infection and colonization by vancomycin-resistant enterococci in critically ill patients. Vancomycin-Resistant Enterococci (VRE) are important causes of Intensive Care Unit (ICU) infections. Our goal was to identify the prevalence and risk factors for VRE colonization upon ICU admission and during ICU stay, as well as, their impact in...