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Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23


, : Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23. Cryobiology 60(3): 271-280

Although cryosurgery is attaining increasing clinical acceptance, our understanding of the mechanisms of cryogenic cell destruction remains incomplete. While it is generally accepted that cryoinjured cells die by necrosis, the involvement of apoptosis was recently shown. Our studies of liver cell death by cryogenic temperature revealed the activation of endonuclease p23 and its de novo association with the nuclear matrix. This finding is strongly suggestive of a programmed-type of cell death process. The presumed order underlying cryonecrotic cell death is addressed here by examining the mechanism of p23 activation. To that end, nuclear proteins that were prepared from fresh liver, which is devoid of p23 activity, were incubated with protein fractions isolated from liver exposed to freezing/thawing that possessed a presumed p23 activation factor. We observed that the activation of p23 was the result of a proteolytic event in which cathepsin D played a major role. Different patterns of proteolytic cleavage of nuclear proteins after in vitro incubation of nuclei and in samples isolated from frozen/thawed liver were observed. Although both processes induced p23 activation, the incubation experiments generated proteolytic hallmarks of apoptosis, while freezing/thawing of whole liver resulted in typical necrotic PARP-1 cleavage products and intact lamin B. As an explanation we offer a hypothesis that after freezing, cells possess the potential to die through necrotic as well as apoptotic mechanisms, based on our finding that the cytosol of cells exposed to cryogenic temperatures contains both necrotic and apoptotic executors of cell death.

US$19.90

PMID: 20132808

DOI: 10.1016/j.cryobiol.2010.01.005


Other references

Courtenay, K., 2002: First episode psychosis. GPs must assess patients' risk behaviour. Bmj 324(7343): 976-976

Biancani, H., 1955: Documentary film on electrokinesia, new method of diagnosis and treatment. Journal de Radiologie, D'electrologie & Archives D'electricite Medicale 36(3-4): 258-258

Tkachuk, M.N., 1982: The oestrous cycle in gilts. Constant visual contact of gilts with a boar lowered the age at which the gilts reached sexual maturity, although the first few oestrous periods were silent. Sexual maturity occurred at a similar age in Russian Large White and Landrace pigs.

Muromtsev V.S., 1989: The applications of electrometric geology to paleogeographic recontructions, mapping and predicting of sand bodies and oil and gas traps. Pages 147-156 1989

Buehler, J., 1993: Nursing in rural Native American communities. This article discusses the importance of cultural sensitivity in providing nursing services to Native Americans. This necessitates general information about cultural universals that transcend specific tribes as well as cultural-specific knowledge....

Ruidavets, J-Bernard.; Bataille, V.; Dallongeville, J.; Simon, C.; Bingham, A.; Amouyel, P.; Arveiler, D.; Ducimetière, P.; Ferrières, J., 2004: Alcohol intake and diet in France, the prominent role of lifestyle. Aim: Moderate alcohol intake is related to a decrease of coronary heart disease. This protective effect may be attributed to ethanol but may also depend on the type of alcoholic beverages. However, these differences may be confounded by lifestyle...

Rhett D.W., 1990: Compaction behavior of North Sea chalk in contact with seawater. Proceedings - Symposium on Rock Mechanics 31: 695-702

Daniels, N.; Sabin, J.E., 2001: What are fairness and consistency in a national pharmacy benefit?. Medical Care 39(4): 312-314

Chirkov V.D., 1970: Importance of conductivity in the spread of epileptiform activity in the cerebral cortex. Biophysics (Engl Transl Biofiz) 15(4): 755-760

Papageorgiou C., 1971: Technique for titrating antibodies inhibiting the growth of mycoplasmata protective antibodies part 1. Bulletin de l'Academie Veterinaire de France 44(10): 471-477