geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proteomic analysis of gemcitabine-induced drug resistance in pancreatic cancer cells


, : Proteomic analysis of gemcitabine-induced drug resistance in pancreatic cancer cells. Molecular Biosystems 7(11): 3065-3074

Currently, the most effective agent against pancreatic cancer is gemcitabine (GEM), which inhibits tumor growth by interfering with DNA replication and blocking DNA synthesis. However, GEM-induced drug resistance in pancreatic cancer compromises the therapeutic efficacy of GEM. To investigate the molecular mechanisms associated with GEM-induced resistance, 2D-DIGE and MALDI-TOF mass spectrometry were performed to compare the proteomic alterations of a panel of differential GEM-resistant PANC-1 cells with GEM-sensitive pancreatic cells. The proteomic results demonstrated that 33 proteins were differentially expressed between GEM-sensitive and GEM-resistant pancreatic cells. Of these, 22 proteins were shown to be resistance-specific and dose-dependent in the regulation of GEM. Proteomic analysis also revealed that proteins involved in biosynthesis and detoxification are significantly over-expressed in GEM-resistant PANC-1 cells. In contrast, proteins involved in vascular transport, bimolecular decomposition, and calcium-dependent signal regulation are significantly over-expressed in GEM-sensitive PANC-1 cells. Notably, both protein-protein interaction of the identified proteins with bioinformatic analysis and immunoblotting results showed that the GEM-induced pancreatic cell resistance might interplay with tumor suppressor protein p53. Our approach has been shown here to be useful for confidently detecting pancreatic proteins with differential resistance to GEM. Such proteins may be functionally involved in the mechanism of chemotherapy-induced resistance.

US$19.90

PMID: 21894339

DOI: 10.1039/c1mb05125c


Other references

Mol, C., 1977: Chinese cabbage under glass. Trials with spring growing. Seeds of Cantonner Witkrop and 5 Japanese hybrid cvs were sown on 6 January at 16 deg C, and the newly emerged seedlings were raised at various temperatures between 12 deg and 24 deg for 5 or 6 weeks. Average plant weight when planted out was high...

Nakamura, E.; Makino, K.; Okano, T.; Yamamoto, T.; Yokoyama, M., 2006: A polymeric micelle MRI contrast agent with changeable relaxivity. Polymeric micelles were formed from cationic polymers (polyallylamine or protamine) and anionic block copolymers (poly(ethylene glycol)-b-poly(aspartic acid) derivative) that bound Gd ions providing high contrasts in Magnetic Resonance Imaging (MR...

Murra, G.; Shane, R., 1995: Evaluating market news and information services. Extension extra 95 (5029)

Crowley, J.G.; Mariano, E.C., 1980: Mesenteric embolectomy. Journal of the Medical Society of New Jersey 77(1): 33-35

Frey, M.J.; Healy, M.; Klinger, G.; Toh, J.; Rucinska, E., 1996: Effect of aspirin on the acute and chronic hemodynamic response to angiotensin II receptor blockade with losartan in heart failure. Journal of the American College of Cardiology 27(2 SUPPL A): 228A

Georgiadis, M.; Cai, Y., 2002: Comprehensive investigation of arsenic speciation in soils and sediments using phosphate solutions as extraction media. Abstracts of Papers American Chemical Society 223(1-2): ANYL 18

Medina, S.H.; El-Sayed, M.E.H., 2009: Dendrimers as carriers for delivery of chemotherapeutic agents. Chemical Reviews 109(7): 3141-3157

Shatz, C., 1977: A comparison of visual pathways in Boston and Midwestern Siamese cats. A genetic mutation in Siamese cats causes retinogeniculate fibers representing roughly the first 20 degrees of ipsilateral visual field in each eye to cross aberrantly in the optic chiasm and terminate in the wrong lateral geniculate nucleus (LGN)...

Goldstein, B.; Fabiano, L.; Iglesias, A., 2004: Spontaneous and imitated productions in Spanish-speaking children with phonological disorders. Research examining the relationship between spontaneous and imitated productions for phonological analysis has indicated that the inclusion of imitated productions may overestimate children's phonological abilities. Previous research in this...

Shigetani, Y.; Ohkura, N.; Yoshiba, K.; Ohshima, H.; Hosoya, A.; Yoshiba, N.; Okiji, T., 2016: GaAlAs laser-induced pulp mineralization involves dentin matrix protein 1 and osteopontin expression. GaAlAs lasers induce pulp mineralization by promoting reparative dentinogenesis. This study analyzed the expression of dentin matrix protein 1 (DMP1) and osteopontin in GaAlAs laser-irradiated rat molars, to examine the hypothesis that these prote...