+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proton-translocating transhydrogenase: an update of unsolved and controversial issues

, : Proton-translocating transhydrogenase: an update of unsolved and controversial issues. Journal of Bioenergetics and Biomembranes 40(5): 463-473

Proton-translocating transhydrogenases, reducing NADP(+) by NADH through hydride transfer, are membrane proteins utilizing the electrochemical proton gradient for NADPH generation. The enzymes have important physiological roles in the maintenance of e.g. reduced glutathione, relevant for essentially all cell types. Following X-ray crystallography and structural resolution of the soluble substrate-binding domains, mechanistic aspects of the hydride transfer are beginning to be resolved. However, the structure of the intact enzyme is unknown. Key questions regarding the coupling mechanism, i.e., the mechanism of proton translocation, are addressed using the separately expressed substrate-binding domains. Important aspects are therefore which functions and properties of mainly the soluble NADP(H)-binding domain, but also the NAD(H)-binding domain, are relevant for proton translocation, how the soluble domains communicate with the membrane domain, and the mechanism of proton translocation through the membrane domain.


PMID: 18972197

DOI: 10.1007/s10863-008-9170-x

Other references

Thomas, J., 1998: Antiphospholipid syndrome-current status. Indian Journal of Dermatology, Venereology and Leprology 64(1): 6-11

Hotokebuchi, N.; Yano, T.; Takeshita, T.; Nishi, K., 1991: Acceleration of H+ extrusion via Na(+)-H+ exchange in guinea-pig ventricular papillary muscle under intracellular acidic condition. We investigated mechanisms by which intracellular pH was regulated under intracellular acidic condition in resting guinea-pig ventricular papillary muscles in vitro. Intracellular sodium ion activity (aiNa), intracellular and surface pH (pHi and p...

T.N.T.les;; R.P.kter, 2011: Statistical mechanics of 1D self-gravitating systems the core–halo distribution. We study, using both theory and simulations, a system of self-gravitating sheets. A new statistical mechanics theory free of any adjustable parameters is derived to quantitatively predict the final stationary state achieved by this system after th...

Štrkalj, G., 2015: The emergence of humanistic anatomy. Medical Teacher 36(10): 912-913

Hoffmann, B.; LaPaglia, S.K.; Kubler, E.; Andermann, M.; Eckert, S.E.; Braus, G.H., 2000: Developmental and metabolic regulation of the phosphoglucomutase-encoding gene, pgmB, of Aspergillus nidulans. We have isolated the pgmB gene from Aspergillus nidulans, which encodes a phosphoglucomutase, one of the key enzymes in carbohydrate metabolism. The pgmB gene is located on chromosome VII and its ORF encodes 557 amino acids. Mutant phenotypes were...

Young, J.B.; Abraham, W.T.; Bourge, R.C.; Konstam, M.A.; Stevenson, L.Warner., 2008: Task force 8: training in heart failure endorsed by the Heart Failure Society of America. Journal of the American College of Cardiology 51(3): 383-389

Steemans, P.; Loboziak, S., 1996: Maurice Streel, an annotated list of his publications. The list of all Maurice Streel's publications is sorted by date. Stratigraphical and geographical distributions of his works are plotted on two figures.

Kieft Thomas L.; Brockman Fred J., 2001: Vadose zone microbiology. Pages 141-169 2001

Khosla, S.; Melton, L.J.; Wermers, R.A.; Crowson, C.S.; O'Fallon, W.m.; Riggs, B.l., 1999: Primary hyperparathyroidism and the risk of fracture: a population-based study. While severe primary hyperparathyroidism (HPT) is clearly associated with osteitis fibrosa cystica, it remains uncertain whether mild, asymptomatic primary HPT adversely affects the skeleton. Thus, we assessed the incidence of age-related fracture...

Peters, A.M.; Walport, M.J.; Bell, R.N.; Lavender, J.P., 1984: Methods of measuring splenic blood flow and platelet transit time with In-111-labeled platelets. Following bolus intravenous injection of In-111-labeled autologous platelets in humans, splenic blood flow (SBF) was measured by compartmental analysis of the equilibration of platelets between blood and the intrasplenic platelet pool. The result...