+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Puerarin attenuates calcification of vascular smooth muscle cells

, : Puerarin attenuates calcification of vascular smooth muscle cells. American Journal of Chinese Medicine 42(2): 337-347

Several studies demonstrate that estradiol can prevent arterial calcification. However, little is known regarding the effect of puerarin, a phytoestrogen extracted from Radix Puerariae, on arterial calcification. The aim of the present study was to determine whether puerarin reduced osteoblastic differentiation of calcifying vascular smooth muscle cells (CVSMCs). The CVSMCs were isolated from mice aorta and treated with different concentrations of puerarin. The alkaline phosphatase (ALP) activity, osteocalcin secretion and Runx2 expression were determined. To examine whether estrogen receptors (ERs) PI3K and Akt play a role in this effect, ICI182789, phosphoinositide 3-kinase (PI3K) inhibitor, LY294002, or the Akt inhibitor, 1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-O-methyl-3-O-octadecylcarbonate (HIMO) was used. Our results showed puerarin could inhibit ALP activity, osteocalcin secretion and Runx2 expression in CVSMCs. Puerarin could induce the activation of Akt. Furthermore, pretreatment of ICI182780, LY294002, HIMO could abolish the effect of puerarin on ALP activity in CVSMCs. Our experiment demonstrated that puerain could attenuate the osteoblastic differentiation of VSMCs through the ER/PI3K-Akt signal pathway.


PMID: 24707866

DOI: 10.1142/S0192415X14500220

Other references

Belotskiĭ, S.M., 1966: Changes in the reactivity of tissue in the process of infection and immunity. Effect of dose, intervals and non-specific factors on the development of influenza infections in mice. Voprosy Virusologii 11(3): 272-277

Shenoy, J.; Kapur, M., 1996: Prevalence of scholastic backwardness among five to eight year old children. The present paper reports the findings of a study of scholastic backwardness among five to eight year old school going children. 1535 children were screened by their class teachers, of which 10.23% were found to have scholastic backwardness. No ge...

Neidhart, M.; Michel, B.A.; Hauselmann, H.J.rg; Dicesara, P.E.; Hauser, N.; Paulsson, M., 1995: Cartilage proteins in synovial fluid and serum as markers for cartilage degradation. Arthritis & Rheumatism 38(9 SUPPL ): S238

Tohen, M.; Waternaux, C.M.; Tsuang, M.T.; Hunt, A.T., 1990: Four-year follow-up of twenty-four first-episode manic patients. Twenty-four first-episode manic patients were followed to investigate the 4-year outcome after recovery from a manic episode. Patients had no documented previous manic or depressive episodes. The presence of psychotic features during the index epi...

Schiel, H.; Jacob, A.; Hammer, B.; Lambrecht, J.T., 1996: Transfer of the dental arch situation into 3D Laser-Technology-Models. Lemke, H U [Author], Vannier, M W [Author], Inamura, K [Author], Farman, A G [Author] International Congress Series; CAR '96: Computer assisted radiology 705-711

Davis, D.L.; Finkner, V.C., 1973: Influence of Temperature on Sterol Biosynthesis in Triticum aestivum. In wheat cv. Monon, the predominant sterol was sitosterol, while cholesterol accounted for < 1% of the sterol in shoots and was present only in trace amounts in roots. Decrease in temperature from 10 to 1 deg C caused a decrease in the sitoster...

Engstrom, W.W., 1952: Some clinical aspects of potassium metabolism. American Practitioner and Digest of Treatment 3(8): 623-625

van Dusseldorp, M., 1997: Classification of folic acid. Lancet 349(9047): 289-289

Verseveldt, J., 1982: New species of Alcyonacea (Octocorallia) from the Great Barrier Reef, south-east Asia, and the Red Sea. Zoologische Mededelingen (Leiden): 5612: 143-151

Cherry J.D.; Soriano F.; Jahn C.L., 1968: Search for peri natal viral infection a prospective clinical virologic and serologic study child echovirus coxsackievirus cytomegalovirus anom congenital rubella. American Journal of Diseases of Children 116(3): 245-250