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Purification and characterization of a new weak hemorrhagic metalloproteinase BmHF-1 from Bothrops marajoensis snake venom

, : Purification and characterization of a new weak hemorrhagic metalloproteinase BmHF-1 from Bothrops marajoensis snake venom. Protein Journal 29(6): 407-416

BmHF-1, from the venom of Bothrops marajoensis, was purified by Sephadex G-75 and HPLC-RP on micro-Bondapak C-18 column chromatography. It presented a molecular mass of 27162.36 Da determined by MALDI-TOF MS. BmHF-1 had a sequence of 238 residues of amino acids. The multiple alignment of its amino acid sequence and those of other snake venom metalloproteinases showed high structural similarity, mainly among P-I class. The enzyme initially cleaves the Aalpha-chain of fibrinogen, followed by the Bbeta-chain, and shows no effects on the gamma-chain. BmHF-1 had, caseinolytic and weakly hemorrhagic activities, which were inhibited by EDTA. In contrast, PMSF did not affect these activities. The caseinolytic activity of BmHF-1 had a pH optimum of 8.0 and was stable in solution up to 40 degrees C; activity was completely lost at > or = 70 degrees C. The proteolytic activity was also inhibited by sDa (opossum sera) and Da2-1, Da2-II, antihemorrhagic factors isolated from the opossum sera of Didelphis albiventris. BmHF-1 presents weak hemorrhagic activity, with a MHD of 41.14 microg and it induces dose-dependent edema. We could concluded that, despite its weak hemorrhagic activity, BmHF-1 contributes to local tissue damage by inducing edema, releasing pharmacologically active mediators from protein precursors due to its enzymatic action.


PMID: 20607373

DOI: 10.1007/s10930-010-9267-z

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Sommerhoff, C.P.; Nadel, J.A.; Basbaum, C.B.; Caughey, G.H., 1990: Neutrophil elastase and cathepsin G stimulate secretion from cultured bovine airway gland serous cells. To investigate the hypothesis that neutrophil proteases stimulate airway gland secretion, we studied the effect of human cathepsin G and elastase on secretion of 35S-labeled macromolecules from cultured bovine airway gland serous cells. Both prote...

Beveridge, A.E.; Franklin, D.; Knowles, B., 1968: Second growth sequence after burning. Studies have been begun to determine how bracken invades burnt ground, recently under indigenous forest cover, in New Zealand. Preliminary investigations showed that germination of spores is definitely induced by the burn, and that in late autumn...

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Belles, X.; Monod, T., 1992: Mezium affine Boieldieu dans les mines de sel de Taoudenni (Sahara Malien) (Coleoptera, Ptinidae). Bulletin de la Societe Entomologique de France, 973: 277-280

Mchugh S.M.; Ewan P.W., 1989: Reduction of increased serum neutrophil chemotactic activity following effective hyposensitization in house dust mite allergy. Changes in the level of serum neutrophil chemotactic activity (S-NCA) were investigated in 20 subjects with allergic rhinitis, with or without asthma, undergoing clinically effective hyposensitization to house dust mite with Pharmalgen Dermatophag...

Wiemann Michael C.; Wheeler Elisabeth A.; Manchester Steven R.; Portier Kenneth M., 1998: Dicotyledonous wood anatomical characters as predictors of climate. The relationships among wood anatomical characters and climate are examined for 50 wood anatomical features in floras from 37 regions in North America, South America, Africa and Malaysia. Correlations, simple regressions and multiple regressions,...

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Gulec, S.A.; Weintraub, S.; Moulder, P.V.; Hunt, J., 2003: 99mTc-sulfur colloid intestinal perforation study using a gamma probe. Journal of Nuclear Medicine 44(5 Supplement): 95P, May

Mattot, M.; Ninane, J.; Cornu, G.; D.N.yer, P., 1989: Is there a place for chemotherapy in the management of recurrent digital fibrous tumor of childhood? A case report. The case of an 11-year-old boy suffering digital fibrous tumor of childhood with multiple recurrences is presented. The possible benefit of chemotherapy instead of a high-level amputation is discussed.