geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover


, : Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover. Bioresource Technology 147: 658-661

A new β-glucosidase (Cel3B) was first isolated from cellulytic fungi, designated as PpCel3B. Although PpCel3B was classified to GH family 3 based on the homology sequence, PpCel3B had different biological functions in cellulose degradation and signaling molecules production. PpCel3B was constitutive and could form multiple soluble lignocellulose inducers for cellulase and hemicellulase synthesis via high tranglycosylation activity and new enzymatic activity. Moreover, PpCel3B showed apparent synergism with cellulases by removing several inhibitors. Supplementing low doses of PpCel3B (52 μg/g substrate) increased saccharification efficiency of cellulase produced by Trichoderma reesei and Penicillium piceum by 15% and 35%, respectively on delignified corn stover. PpCel3B had important application in boosting cellulase yield and efficiency.

US$19.90

PMID: 24025854

DOI: 10.1016/j.biortech.2013.08.089


Other references

Yücel, M.A.; Selb, J.; Boas, D.A.; Cash, S.S.; Cooper, R.J., 2014: Reducing motion artifacts for long-term clinical NIRS monitoring using collodion-fixed prism-based optical fibers. As the applications of near-infrared spectroscopy (NIRS) continue to broaden and long-term clinical monitoring becomes more common, minimizing signal artifacts due to patient movement becomes more pressing. This is particularly true in application...

Wang, Y.; Zhou, L.; Yu, X.; Stover, E.; Luo, F.; Duan, Y., 2016: Transcriptome Profiling of Huanglongbing (HLB) Tolerant and Susceptible Citrus Plants Reveals the Role of Basal Resistance in HLB Tolerance. Huanglongbing (HLB) is currently the most destructive disease of citrus worldwide. Although there is no immune cultivar, field tolerance to HLB within citrus and citrus relatives has been observed at the USDA Picos farm at Ft. Pierce, Florida, whe...

Ginsburg, G.S.; Small, D.M.; Hamilton, J.A., 1982: Temperature-dependent molecular motions of cholesterol esters: a carbon-13 nuclear magnetic resonance study. Carbon-13 NMR spectroscopy at 50.3 MHz has been used to study four long-chain cholesterol esters with a double bond in the omega-9 position: cholesteryl oleate, C18:1, omega-9; cholesteryl linoleate, C18:2, omega-6,9; cholesteryl erucate, C22:1, o...

Martin, N.F., 1984: Carbonic anhydrase inhibitors for prevention of space motion sickness: an avenue of investigation. Space motion sickness (SMS) is an important medical problem facing NASA's space shuttle program. Two theories that have been advanced to explain SMS are the "fluid shift theory" and the "vestibulo-ocular sensory conflict theory...

Ercan, Z.S.; Torunoğlu, M.; Türker, R.K., 1980: Elaboration of prostaglandin-like material from isolated perfused rabbit spleen by angiotensin II. New Istanbul Contribution to Clinical Science 13(1): 3-8

Pearce, P., 2016: Preparing to care for paediatric trauma patients. Considerable evidence has discussed the significant workload and advances in clinical care by UK Defence Medical Services (DMS) during recent conflicts in Iraq and Afghanistan. Although the DMS is not doctrinally staffed to deal with children on o...

L.Z.xian; Liu Gang; Han SiHuai; Chen XueLiang, 1996: Study on the cytogenetics of the polyploidy in O. sativa x O. grandiglumis F2 plants. The chromosome number of F2 plants of the cross Oryza sativa x O. grandiglumis was 2n = 4x = 48, revealing that the allotriploid of hybrid F1 had been altered into allotetraploid in the F2. Chromosome behaviour was studied at meiosis and revealed...

Peng, L.; Xu, Z.; Fang, X.; Wang, F.; Yang, S.; Cen, P., 2004: Preferential codons enhancing the expression level of human beta-defensin-2 in recombinant Escherichia coli. Human beta-defensin-2 (hBD2) is a small antimicrobial peptide with potential as a therapeutic agent. The effect of codon usage on the expression of hBD2 in Escherichia coli was studied. Two coding sequences encoding the same hBD2 precursor were bo...

Trakhtenberg I.M.; Korshun M.N., 1978: Substantiating the average per shift maximum permissible concentrations of highly cumulative noxious substances in the air of the production area. Gigiena Truda i Professional'nye Zabolevaniya (1): 14-18

Lerner, M.; Stopalov, S.; Poliak, A.; Frankshtein, M., 1981: Technical-economic criteria of the critical state of a tractor. Traktory i sel'khozmashiny: ) 28-30