geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India


, : Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India. Indian Journal of Experimental Biology 46(8): 573-578

There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes. Five out of twenty clinical samples were positive for CHIK virus. The partial sequences of the E1 and nsP1 genes of the strain, IndKL01 were highly similar to the Reunion strains and the recently isolated Indian strains. A novel substitution, A148V, was detected in the E1 gene of the isolate, IndKL02. The detection procedure used in this study was simple, sensitive and rapid (less than 4 hr). This result suggests that CHIK viruses similar to the Reunion strains, which had resulted in high morbidity and mortality rates, may have caused the recent Chikungunya outbreak in India. The effect of the variant, E1-A148V, in the virulence and the rate of transmission of the virus deserves further investigation.

US$29.90

PMID: 18814485


Other references

McLean, D.T.F.; Lundy, F.T.; Timson, D.J., 2013: IQ-motif peptides as novel anti-microbial agents. The IQ-motif is an amphipathic, often positively charged, α-helical, calmodulin binding sequence found in a number of eukaryote signalling, transport and cytoskeletal proteins. They share common biophysical characteristics with established, catio...

Clemants, Steven E., 1996: International registration of cultivar names for unassigned woody genera 1995. Hortscience 31(3): 326

Li, Y.; Hotta, M.; Shi, A.; Li, Z.; Yin, J.; Guo, G.; Kawata, K.; Ushijima, H., 2007: Malnutrition improvement for infants under 18 months old of Dai minority in Luxi, China. Background: Child malnutrition and thiamine deficiency remain a matter of public concern in Dai children under 5 years old in Southwest areas of China. The aim of the present study was to understand the status and correlates of malnutrition and t...

Rohrs, W.; Wilkens, D., 1984: Force measurements on tillage equipment. The development of various systems is described for measuring the forces acting between tractor and implement and the forces acting directly on the implement. Three categories may be identified: devices to measure traction (1-dimensional), devices...

Gbelcová, H.; Bakeš, P.; Priščáková, P.; Šišovský, V.; Hojsíková, I.; Straka, Ľ.; Konečný, M.; Markus, J.; D'Acunto, C.W.; Ruml, T.; Böhmer, D.; Danihel, Ľ.; Repiská, V., 2016: PTEN sequence analysis in endometrial hyperplasia and endometrial carcinoma in Slovak women. Phosphatase and tensin homolog (PTEN) is a protein that acts as a tumor suppressor by dephosphorylating the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. Loss of PTEN function has been implicated in the pathogenesis of a number...

Gill, G.V., 1979: Mushroom poisoning in Zambia. Fourteen cases of poisoning are described of which 7 (caused by Lepiota morgani) were in 2 families of 3 and 4 people, respectively. Two patients among the 14 died and the rest suffered gastrointestinal disturbance of variable intensity. Amanita p...

Sanchez Palacios, M.; Trevino Pena, E.; Garcia Oliva, I.; Martin Gonzalez, J.C.; Perez Ortiz, J.B.anco Lopez And C., 1997: Mechanical ventilation does not increase the mortality in asthmatic patients. Journal of Investigational Allergology & Clinical Immunology 7(5): 442, Sept -Oct

Zalimbekov, Zg, 1971: Soil grouping in agricultural regions of Dagestan. Pochvovedenie 7 3-12

Kositsyna Pinegina, E.A., 1989: Production of red clover breeding material with increased seed yield. In 3 sets of trials, with 7, 20 and 40 accessions, material (10 accessions in all) was selected for high percentage of 2-seeded pods/inflorescence, including Wega E2 (15.41%), Lucrum (17.52%), Lutea (14.74%) and (in the largest trial) Bijorn (37.3...

Kobara, R., 1969: Effects of food plants on the hemolymph protein of the fall webworm, Hyphantria cunea. When the haemolymph proteins in larvae of Hyphantria cunea (Dru.) that had developed from eggs collected from different food-plants in Japan were investigated by agarose-gel electrophoresis, the patterns obtained showed three main fractions (refer...