geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Recognition based hormonal 95kDa monoclonal antibody on three human cancer cell lines for developing targeted radio-immuno-imaging and therapy


, : Recognition based hormonal 95kDa monoclonal antibody on three human cancer cell lines for developing targeted radio-immuno-imaging and therapy. Hellenic Journal of Nuclear Medicine 15(2): 108-113

The present study aimed to explore the possibility of developing a immuno-imaging/therapeutic agent for hCG-expressing tumors by using antibodies raised against them. Three human cancer cell lines were selected:lung adenocarcinoma (A549), glioblastoma (U87MG) and breast cancer (MCF7). Anti-β-hCG monoclonal antibody, obtained from ascitic fluid, was purified by affinity chromatography followed by characterization and titration. Ectopic expression of hCG on these cell lines was tested by flow cytometry and in-vitro cytotoxicity with antibodies was tested by MTT assay on the cell line with the highest percentage binding. For positive and negative controls, immortalized trophoblast cells (SW71) and peripheral blood monocytes were used. Antibody was then radiolabeled with lutetium-177 ((177)Lu) and in vivo biodistribution studies were conducted in murine tumor model. Antibodies could be purified to homogeneity with a concentration 28mg/mL. Percentage receptor expression on A549, U87MG and MCF7 cells was 95%, 66% and 55% respectively. About 90% of A549 cells could be killed with antibody at 72h post-treatment. No cytotoxicity was observed on SW71 despite a high binding percentage (96%). Antibodies were radiolabeled with high efficiency (∼98%). In-vivo studies using radiolabeled antibodies showed hepato-biliary excretion route and significant uptake in A549 tumor. In conclusion, among the 3 cancer cell lines, lung adenocarcinoma significantly expresses β-hCG and shows dose dependent cytotoxicity with anti-β-hCG antibody. Radiolabeling of this antibody can aid in imaging and also has the potential of enhancing its therapeutic potential. This study provides a platform for further studies for targeted radio-immuno imaging and subsequent therapy of hCG-expressing cancers.

US$29.90

PMID: 22833856


Other references

Chabin, M., 1983: Hospital-supported child care. American Journal of Nursing 83(4): 548-552

Liscia A.; Crnjar R.; Angioy A.M.; Barbarossa I.T., 1989: Gtp and cyclic gmp both enhance while cyclic amp depresses the response to a furanose sugar of taste chemosensilla in the blowfly protophormia terraenovae. 1. The stimulatory effect of a fructose (furanose sugar) solution on labellar taste chemosensilla in the blowfly Protophormia terraenovae is enhanced in the presence of GTP or cGMP. 2. The increase in stimulating effectiveness due to cGMP is doubl...

Mayo-Smith, M.F., 1987: Type non-A, non-B and type B hepatitis transmitted by a single needlestick. American Journal of Infection Control 15(6): 266-267

Deminatti, M., 1962: Comparative histoautoradiographical study of the anterior pituitary gland of Carassium auratus after administration of S35-labeled Na2SO4 and S35-labeled methionine. Comptes Rendus Hebdomadaires des Seances de L'academie des Sciences 254: 1510-1511

Alexander, G.; Lynch, J.J.; Mottershead, B.E., 1979: Use of shelter and selection of lambing sites by shorn and unshorn ewes in paddocks with closely or widely spaced shelters. In late winter sheep spent more time sheltering behind phalaris hedges set 20 m apart than behind those set 240 m apart, but spent more time behind the latter than behind plastic mesh screens set at the same distance. About half the ewes isolated...

Mcmillian W.W.; Widstrom N.W.; Wilson D.M., 1987: Impact of husk type and species of infesting insects on aflatoxin contamination in preharvest corn at tifton georgia usa. The use of plants that resist insects has been suggested as a potential means of reducing aflatoxin contamination in some crops. Dent corn, Zea mays L., germplasm possessing the characteristic of a relatively tight, complete husk cover and germpla...

Schmitz Lars, 2003: Fish teeth (Neoselachii; Actinopterygii) from the lower Barremian of northwestern Germany. Neues Jahrbuch fuer Geologie und Palaeontologie Abhandlungen

Koontz, D.A.; Choi, J.H., 1993: Evidence for phosphorylation of tubulin in carrot suspension cells. Two dimensional gels of phosphoproteins from carrot (Daucus carota L. var. Juwarot) suspension cells labeled in vivo or in vitro revealed phosphoproteins that co-migrate with carrot tubulin. A polyclonal antiserum to hibiscus tubulin immunoprecipi...

Brotherton, J.M.L.; Leask, J.; Jackson, C.; McCaffery, K.; Trevena, L.J., 2010: National survey of general practitioners' experience of delivering the National Human Papillomavirus Vaccination Program. Between 2007 and 2009, Australian general practitioners (GPs) were involved in implementing a population-based human papillomavirus (HPV) vaccination program. We investigated GPs' experiences of delivering the HPV vaccine to women aged 18-26....

Schneider M.; Wenzel M., 1982: Labeling acetyl ruthenocene with ruthenium 97 deuterium or tritium. Acetylruthenocene was labeled by heating acetylferrocene with 97RuCl3. The 3H-labeled compound was gained by exchange in 3H20 at the acetyl side-chain of acetylruthenocene. In a similar way acetylferrocene was labeled with deuterium.