+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Regions outside the alpha-crystallin domain of the small heat shock protein Hsp26 are required for its dimerization

, : Regions outside the alpha-crystallin domain of the small heat shock protein Hsp26 are required for its dimerization. Journal of Molecular Biology 398(1): 122-131

Small heat shock proteins (sHsps) are a ubiquitous family of molecular chaperones. They form homo-oligomers, composed of mostly 24 subunits. The immunoglobulin-like alpha-crystallin domain, which is flanked by N- and C-terminal extensions, is the most conserved element in sHsps. It is assumed to be the dimeric building block from which the sHsp oligomers are assembled. Hsp26 from Saccharomyces cerevisiae is a well-characterized member of this family. With a view to study the structural stability and oligomerization properties of its alpha-crystallin domain, we produced a series of alpha-crystallin domain constructs. We show that a minimal alpha-crystallin domain can, against common belief, be monomeric and stably folded. Elongating either the N- or the C-terminus of this minimal alpha-crystallin domain with the authentic extensions leads to the formation of dimeric species. In the case of N-terminal extensions, their population is dependent on the presence of the complete so-called Hsp26 "middle domain". For the C-terminal extensions, the presence of the conserved IXI motif of sHsps is necessary and sufficient to induce dimerization, which can be inhibited by increasing ionic strength. Dimerization does not induce major changes in secondary structure of the Hsp26 alpha-crystallin domain. A thermodynamic analysis of the monomeric and dimeric constructs revealed that dimers are not significantly stabilized against thermal and chemical denaturation in comparison to monomers, supporting our notion that dimerization is not a prerequisite for the formation of a well-folded Hsp26 alpha-crystallin domain.


PMID: 20171228

DOI: 10.1016/j.jmb.2010.02.022

Other references

Naeymi Rad, F.; Trace, D.; Carmony, L.; Evens, M.; Juang, M.; D.S.uza Almeida, F.; Kepic, T.; Roberts, R.; Zelanski, R.; Weil, M.H., 1992: Steps in implementing a multiuser integrated electronic medical record. Lun, K C [Editor], Degoulet, P [Editor], Piemme, T E [Editor], Rienhoff, O [Editor] IFIP (International Federation for Information Processing) World Conference Series on Medical Informatics, Vol 7 Parts 1 and 2 ; MEDINFO 92 : liv+858p (Part 1), xxx+779p (Part 2) 724-729

Chadha, S.K., 1992: Problems of animal grazing in Himalayas-a case history of Uttar Pradesh hills. The problems caused by uncontrolled grazing on pasture production are discussed and measures to address these problems are proposed.

Hanneberg, P., 1984: The Seychelles - an oceanic nature paradise.. Sveriges Natur, 756: 3-7

Berkowitz, F.E.; Severens, J.L.; Blumberg, H.M., 2006: Exposure to tuberculosis among newborns in a nursery: decision analysis for initiation of prophylaxis. Objective. Newborns in a hospital nursery were exposed to a mother whose sputum was direct-smear negative for acid-fast bacilli but culture positive for Mycobacterium tuberculosis. Given the low risk for exposure, the high susceptibility of infan...

Asami T., 1988: Soil population by metals from mining and smelting. Salomons, W And U Foerstner (Ed ) Chemistry And Biology Of Solid Waste: Dredged Material And Mine Tailings X+305p Springer-Verlag, New York, Inc : Secaucus, New Jersey, Usa; Berlin, West Germany Illus Maps 143-169

Romanchishen, A.F.; Vabalayte, K.V.; Tovbina, M.H., 2011: The story of an old photograph. Surgery 149(3): 452-454

Claas F.H.J.; Gijbels Y.; Veen A.V.; L.P.; D'amaro J.; Persijn G.G.; Van Rood J.J., 1989: Selection of cross match negative hla a and or hla b mismatched donors for highly sensitized patients. Transplantation Proceedings 21(1 PART 1): 665-666

Neuman, M., 1986: Broad-spectrum quinolones (2d-generation quinolones). Therapie 41(2): 123-128

Liu, W.; Ackermann, L., 2014: Versatile ruthenium(II)-catalyzed C-H cyanations of benzamides. Direct cyanations of arenes and heteroarenes bearing only weakly coordinating amides were accomplished using a robust ruthenium(II) catalyst. The user-friendly C(sp(2))-H activation occurred with the assistance of carboxylate with high site-select...

Robin, Y., 1953: Biochemical preparation of gamma-guanidobutyric acid and its significance in the metabolism of guanidine derivatives. Bulletin de la Societe de Chimie Biologique 35(3-4): 285-288