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Retinal self-antigen induces a predominantly Th1 effector response in Axl and Mertk double-knockout mice


, : Retinal self-antigen induces a predominantly Th1 effector response in Axl and Mertk double-knockout mice. Journal of Immunology 187(8): 4178-4186

The TAM family of receptors (Tyro3, Axl, and Mertk) plays an important role in the negative regulation of response of dendritic cells (DCs) and macrophages to pathogenic stimuli, and mice lacking this receptor family develop spontaneous lupus-like systemic autoimmunity against a variety of tissues, including retina. To study the molecular mechanism underlying the TAM regulation of APC functions and subsequent effects on the induction of an autoimmune response against the eye, we examined CD4 T cell differentiation following retinal self-antigen immunization. CD4 T cells prepared from naive or interphotoreceptor retinoid-binding protein (IRBP)1-20-immunized Axl and Mertk double-knockout (dko) mice reacted to activation using anti-CD3 and anti-CD28 Abs or to bolster by self-antigen in vitro with a predominantly Th1 effector response, as characterized by increased IFN-γ production and higher frequency of IFN-γ-positive CD4 T cells. The Th17 effector response to IRBP immunization was similar in dko mice to that in wild-type controls, as shown by ELISA measurement of IL-17A in the culture medium and flow cytometric analysis of IL-17A-secreting CD4 T cells. Interestingly, APCs or DCs isolated from IRBP-immunized dko mice exhibited a greater ability to drive the Th1 response. The production of two driving cytokines for Th1 differentiation, IL-12 and IL-18, was dramatically increased in dko DCs and macrophages, and LPS stimulation bolstered their production. The preferential development into the Th1 subset in dko mice suggests that the cytokine milieu produced by the mutant mice in vivo or by mutant APCs in vitro selectively creates a differentiation environment favoring the Th1 effector response.

US$19.90

PMID: 21918185

DOI: 10.4049/jimmunol.1101201


Other references

Lynch, D.V.; Phinney, A.J., 1995: The transbilayer distribution of glucosylceramide in plant plasma membrane. Kader, J -C [Editor], Mazliak, P [Editor] Plant lipid metabolism 239-241

Cunningham, K.I.; Northup, D.E.; Pollastro, R.M.; Wright, W.G.; Larock, E.J., 1995: Bacteria, fungi and biokarst in Lechuguilla Cave, Carlsbad Caverns National Park, New Mexico. Lechuguilla Cave is a deep, extensive, gypsumand sulfur-bearing hypogenic cave in Carlsbad Caverns National Park, New Mexico, most of which (>90%) lies more than 300 m beneath the entrance. Located in the arid Guadalupe Mountains, Lechuguilla&a...

Li, H.; Zhu, W.; Xia, M., 1997: Detection of human papillomavirus DNA in condylomata acuminata treated with CO2 laser by polymerase chain reaction. Chinese Medical Journal 110(1): 78-80

Ali, A.; Hayder, M., 2008: Seasonal variation of reproductive performance, foetal development and progesterone concentrations of sheep in the subtropics. The aim of the present study was to investigate the effect of breeding season [late spring (LSP) vs winter (WIN)] on reproductive performances, foetal development and progesterone (P(4)) concentrations of sheep in the subtropics. Ewes of Farafra b...

Lewis D.L.; Holm H.W.; Kollig H.P.; Hall T.L., 1984: Transport and fate of di ethyl phthalate in aquatic ecosystems. Sorption, alkaline hydrolysis, photolysis and microbial transformation of diethyl phthalate (DEP) were studied in a large laboratory aquatic ecosystem composed of a series of reactor tanks. Microbial transformation accounted for 95-99% of the rapi...

Ertas, F.; Karaoguz, R.; Guldal, M.; Kumbasar, D.; Gulec, S.; Oral, D., 2000: Atrial sensing performance of a single-lead VDD pacing system during physical activities. This study was undertaken to evaluate the atrial sensing performance of a single-lead atrial synchronous-ventricular pacing system during various physical activities on late follow-up. Fourteen patients (mean age 55 +/- 17 years) with a third-degr...

Knowles, D.W.; Biggin, M.D., 2014: Building quantitative, three-dimensional atlases of gene expression and morphology at cellular resolution. Animals comprise dynamic three-dimensional arrays of cells that express gene products in intricate spatial and temporal patterns that determine cellular differentiation and morphogenesis. A rigorous understanding of these developmental processes r...

Ryabinin A.N. (Riabinin), 1937: Notice sur les restes d'un lion des cavernes des environs de la ville de Sotchi sur la riviera de la mer Noire. Note on remains of Felis leo spelaea from a middle Pleistocene terrace deposit near the village of Sochi on the shore of the Black sea, Russia.

Sharma, T.; Coutinho, O., 1978: A spatio temporal analysis of wheat crop in Uttar Pradesh, 1960-61 to 1974-75. Agricultural situation in India: 33 (2) 81-86

Eungdamrong, J.; Boyd, K.P.; Meehan, S.A.; Latkowski, J-Ann., 2014: Muckle-Wells treatment with anakinra. A 35-year-old man initially was referred for management of recalcitrant urticaria. Owing to his long history of arthritis and sensorineural hearing loss, genetic testing was performed. The test showed a D305N heterozygous mutation in the NLRP3 gen...