+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Rituximab treatment in hepatitis C infection: an in vitro model to study the impact of B cell depletion on virus infectivity

, : Rituximab treatment in hepatitis C infection: an in vitro model to study the impact of B cell depletion on virus infectivity. Plos One 6(9): E25789-E25789

Hepatitis C virus (HCV) infected patients with vasculitis are often treated with the B-cell-depleting anti-CD20 antibody rituximab. Treatment reduces the cryoglobulins that cause vasculitis, yet it also leads to a transient increase in liver enzymes and HCV genomic RNA in the periphery. The mechanism underlying the increased viral load is unclear and both direct and indirect roles have been proposed for B cells in HCV infection. We previously reported that HCV can associate with B cells and can trans-infect hepatocytes. We established an in vitro assay to study the effect(s) of rituximab on B cell-associated HCV infectivity. Rituximab-mediated lysis of B cells in vitro increases the level of infectious HCV released from B cells. Our results, using a model where virus does not replicate in B cells, recapitulate observations seen in patients and may explain in part the rapid increase in blood HCV RNA observed after rituximab treatment.


PMID: 21991396

DOI: 10.1371/journal.pone.0025789

Other references

Hayes, R.P.; Lewis, K.M.; Xun, L.; Kang, C., 2013: Catalytic mechanism of 5-chlorohydroxyhydroquinone dehydrochlorinase from the YCII superfamily of largely unknown function. TftG, 5-chloro-2-hydroxyhydroquinone (5-CHQ) dehydrochlorinase, is involved in the biodegradation of 2,4,5-trichlorophenoxyacetate by Burkholderia phenoliruptrix AC1100. It belongs to the YCII superfamily, a group of proteins with largely unknown...

Mock, T.; Kruse, M.; Dieckmann, G.S., 2003: A new microcosm to investigate oxygen dynamics at the sea ice water interface. A laboratory sea ice microcosm was developed to enable the cultivation of the ice diatom Fragilariopsis cylindrus in the skeletal layer and bottom 10 cm of sea ice. Growth of diatoms was ensured by continuous flow of new medium beneath the ice. Li...

Lacy, M.L., 1977: Influence of chemical treatments on stand establishment in asparagus. Seed treatment with captan, thiram and other fungicides did not improve seedling establishment in soil infested with Fusarium oxysporum f. sp. asparagi and F. moniliforme [Gibberella fujikuroi], whether fumigated with Vorlex [methyl isothiocyanate...

Jaynes M.L.; Carpenter S.R., 1985: Effects of submersed macrophytes on phosphorus cycling in surface sediment. Proceedings of the Annual Conference & International Symposium of the North American Lake Management Society 4: 370-374

Al-Bagdadi,, C., 1988: Ultrastructural morphology of the epidermis of the dog with generalized demodectic mange. Proceedings annual meeting Electron Microscopy Society of America: 6) 368-369

Bush, W.W., 1999: Overview of transplantation immunology and the pharmacotherapy of adult solid organ transplant recipients: focus on immunosuppression. A review of transplantation immunology is discussed with emphasis on alloantigen presentation, T-lymphocyte activation and proliferation, and the immune effector mechanisms responsible for allograft rejection. Immunosuppressive pharmacology is int...

Schmidt, K.L., 1980: Rehabilitation prognosis of ankylosing spondylitis. Development trends with reference to current scientific knowledge. Lebensversicherungs Medizin 32(4): 89-92

Li, W-Chang.; Ye, S-Long.; Sun, R-Xia.; Liu, Y-Kun.; Tang, Z-You.; Kim, Y.; Karras, J.G.; Zhang, H., 2006: Inhibition of growth and metastasis of human hepatocellular carcinoma by antisense oligonucleotide targeting signal transducer and activator of transcription 3. Hepatocellular carcinoma (HCC) is an aggressive malignancy and is a devastating clinical complication of chronic liver disease. Therapeutic options are limited mainly because the genetic and biochemical understanding of this disease remains fragme...

Wunderlich, J., 2004: On the relationships of the families of the superfamily Araneoidea (Araneae) and their kin, with cladograms, remarks on the origin of the orb web, and description of the new and extinct families Baltsuccinidae and Protheridiidae in Tertiary Baltic amber. With some hesitation the superfamily Araneoidea (Araneae) is regarded in a wide sense including the cribellate families Deinopidae and Uloboridae (= "Orbiculariae"). Preliminary cladograms of the families of the Araneoidea and their kin...

Rypacek, V., 1967: Biology of wood-destroying fungi. This comprehensive monograph, based on world literature and original Czech research, is a Russian translation of the Czech edition, revised and enlarged by the author . It contains the following chapters: the main wooddestroying fungi, their repro...