+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Screening, identification, and potential interaction of active compounds from Eucommia ulmodies leaves binding with bovine serum albumin

, : Screening, identification, and potential interaction of active compounds from Eucommia ulmodies leaves binding with bovine serum albumin. Journal of Agricultural and Food Chemistry 60(12): 3119-3125

The aqueous extract of Eucommia ulmoides leaves has been commonly known as Du-zhong tea as a functional health food for the treatment of hypertension, hypercholesterolemia, and fatty liver. This study developed a centrifugal ultrafiltration-high-performance liquid chromatography (HPLC) method for screening and identification of bioactive compounds in E. ulmoides leaves binding with bovine serum albumin (BSA). Six active compounds were screened, isolated, and elucidated by their ultraviolet (UV), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) data as geniposidic acid (1), caffeic acid (2), chlorogenic acid (3), quercetin-3-O-sambubioside (4), rutin (5), and isoquercitrin (6). The interaction between active compounds and BSA was investigated in the absence and presence of other compounds by quenching the intrinsic BSA fluorescence. The results indicated that the structures significantly affected the binding process. The values of binding constants for compounds 2-6 were in the range of 10(5)-10(6) mol L(-1), while geniposidic acid (1) hardly quenching the BSA intrinsic fluorescence. However, the quenching process of geniposidic acid was easily affected in the presence of other active compounds. The formation of the geniposidic acid-phenylpropanoid (flavonoid) complex could increase the binding affinity of geniposidic acid with BSA; however, the increased steric hindrance of the complex may make phenylpropanoid or flavonoid dissociate from BSA and then decrease their affinities.


PMID: 22385161

DOI: 10.1021/jf205135w

Other references

Huerkamp, B.; Rittinghaus, F.W., 1950: The blood supply of the human retina under the influence of a varying oxygen pressure carbonic, hyperventilation, and adrenalin. Pflugers Archiv für die Gesamte Physiologie des Menschen und der Tiere 252(3): 312-330

Jyotheeswaran, S.; Shah, A.N.; Jin, H.O.; Potter, G.D.; Ona, F.V.; Chey, W.Y., 1998: Prevalence of Helicobacter pylori in peptic ulcer patients in greater Rochester, NY: is empirical triple therapy justified?. Among patients with peptic ulcer disease, the prevalence of Helicobacter pylori has been reported to range from 80% to 90%. Thus empirical cost-effective therapy has been suggested. We surveyed patients with peptic ulcer disease in Rochester, NY....

Zucchelli, S.; Codrich, M.; Marcuzzi, F.; Pinto, M.; Vilotti, S.; Biagioli, M.; Ferrer, I.; Gustincich, S., 2011: TRAF6 promotes atypical ubiquitination of mutant DJ-1 and alpha-synuclein and is localized to Lewy bodies in sporadic Parkinson's disease brains. Parkinson's disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons in the Substantia Nigra and the formation of ubiquitin- and alpha-synuclein (aSYN)-positive cytoplasmic inclusions called Lewy bodies (LBs)...

Young, T., 2002: Why wound pain should be accurately assessed. Community Nurse 6(10): 41-2, 45

Paepin, Jacques, 1988: A fare for the heart. The videotape program demonstrates how to prepare several dishes from the accompanying recipe book to have a complete low-cholesterol, low-fat but not ho-hum menu. The recipe book's recipes meet the dietary guidelines for health, heart nutrit...

Cruse, G.; Cockerill, S.; Bradding, P., 2008: IgE alone promotes human lung mast cell survival through the autocrine production of IL-6. Background: Mast cells play a key role in asthma and recent evidence indicates that their ongoing activation in this disease is mediated, in part, via IgE in the absence of antigen. In this study we have examined whether IgE alone enhances human...

Vourakis, C., 2016: Editor's Note. Journal of Addictions Nursing 27(4): 226-226

Larbouret, C.; Gaborit, Nège.; Poul, M-Alix.; Pèlegrin, Aé.; Chardès, T., 2015: The HER3/ERBB3 receptor: the dark side of the ERBB planet. Medecine Sciences 31(5): 465-468

Schmidt, E., 1933: Our experiences in the breeding of Phytophthora-resistant Potatoes. In 1925 a number of tubers of the so-called 'SW' potato strain were supplied by the Biological Institute, Berlin-Dahlem, to the v. Kameke seed selection establishment at Streckenthin. According to Muller, the strain in question is derive...

Bordeleau, L.M.; Antoun, H.; Lachance, R.A., 1977: Effects of Rhizobium meliloti isolates and of successive cutting of lucerne (Medicago sativa) on symbiotic N fixation. Symbiotic N fixation with 49 isolates of R. meliloti in lucerne cv. Saranac was studied. It was shown that plant DM yield could be used as an indirect measurement of N fixation and as a criterion for selecting efficient strains of R. meliloti. Sta...