geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Selection of reference genes for real-time PCR in human hepatocellular carcinoma tissues


, : Selection of reference genes for real-time PCR in human hepatocellular carcinoma tissues. Journal of Cancer Research and Clinical Oncology 134(9): 979-986

To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC). Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination. All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expression levels varied with hepatitis infection status. The combination of HPRT and TBP expression levels were the most stable, regardless of differences in tumor stage and cirrhotic and malignancy status. It is necessary to select reference genes based on tissue and disease specific expression profile and to further identify novel reference genes with greater expression stability for use in HBV related HCC gene expression studies.

US$19.90

PMID: 18317805

DOI: 10.1007/s00432-008-0369-3


Other references

Ozdemir, C.; Sökücü, S.Nedime.; Karasulu, L.; Altın, S.; Dalar, L., 2014: Tracheomalacia treatment using a large-diameter, custom-made airway stent in a case with mounier-kuhn syndrome. Mounier-Kuhn Syndrome (MKS) is a rare congenital disease that presents with abnormal enlargement in the central airways. In MKS, tracheomegaly is accompanied by difficulty in expelling recurrent lung infections and bronchiectasia. We presented a p...

Perpiña, M.; Palau, M.; Cortijo, J.; Fornas, E.; Ortiz, J.L.; Morcillo, E., 1989: Sources of calcium for the contraction induced by various agonists in trachealis muscle from normal and sensitized guinea pigs. Active sensitization of guinea pigs resulted in an increase in the responsiveness and sensitivity of tracheal strips to CaCl2 in K+-depolarized tissue (Emax 0.81 +/- 0.22 g/mm2 and pD2 2.35 +/- 0.10 in normal vs. 0.98 +/- 0.01 g/mm2 and 3.10 +/- 0...

Sparkes, R.S.; Mohandas, T.; Sparkes, M.C.; Shulkin, J.D., 1978: Aconitase (E.C. 4.2.1.3) mitochondrial locus (ACONM) mapped to human chromosome 22. Cytogenetics and Cell Genetics 22(1-6): 226-227

Patel, U.H.; Patel, P.D.; Thakker, N., 2007: C--H...O hydrogen-bonding and C--H...pi interactions in 3-(4-fluorophenyl)-1,5,7-trimethyl-1,2,3,4-tetrahydropyrido[2,3-d]pyrimidine-2,4-dione. In the title compound, C(16)H(14)FN(3)O(2), a diverse set of weak intermolecular C--H...pi, pi-pi and C--H...O interactions link the molecules into sheets. The C--H...O interactions generate centrosymmetric rings with a graph-set motif of R(2)(2)(...

Anonymous, 1995: From the Centers for Disease Control and Prevention. Update: HIV-2 infection among blood and plasma donors--United States, June 1992-June 1995. JAMA 274(13): 1007-1008

Sano T.; Ogawa M.; Nakajima T.; Matsushita T.; Kayatani F.; Nakano S.; Matsuda H.; Shimazaki Y.; Taniguchi K.; E.A., 1989: Ventricular function and its reserve in patients after the fontan operation analysis of the response to acute pressure overload. Japanese Circulation Journal 53(8): 920

Smith, A.E.; Matthews, R.G., 2000: Protonation state of methyltetrahydrofolate in a binary complex with cobalamin-dependent methionine synthase. N5-Methyltetrahydrofolate (CH3-H4folate) donates a methyl group to the cob(I)alamin cofactor in the reaction catalyzed by cobalamin-dependent methionine synthase (MetH, EC 2.1.1.3). Nucleophilic displacement of a methyl group attached to a tertiar...

Goldsmith, H.J.; Ahmad, R.; Raichura, N.; Lal, S.M.; McConnell, C.A.; Gould, D.A.; Gyde, O.H.; Green, J., 1983: Association between rising haemoglobin concentration and renal cyst formation in patients on long term regular haemodialysis treatment. Seventeen of 20 (85%) patients on RDT for six to 13 years were found to have acquired renal cysts. There was a significant positive association between Hb and duration of RDT. The extent of cyst was positively and significantly correlated with the...

Krejcí, J.; Pacherník, J.; Hampl, A.; Dvorák, P., 2008: In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles. Labelling of mammalian cells with superparamagnetic iron oxide (SPIO) nanoparticles enables to monitor their fate in vivo using magnetic resonance imaging (MRI). However, the question remains whether or not SPIO nanoparticles affect the phenotype...

Büchner, T.; Hiddemann, W.; Berdel, W.; Wörmann, B.; Schoch, C.; Löffler, H.; Haferlach, T.; Schumacher, A.; Staib, P.; Balleisen, L.; Grüneisen, A.; Rasche, H.; Aul, C.; Heyll, A.; Lengfelder, E.; Ludwig, W-Dieter.; Maschmeyer, G.; Eimermacher, H.; Karow, J.; Frickhofen, N.; Hirschmann, W-Dietrich.; Sauerland, M-Cristina., 2002: Acute myeloid leukemia: treatment over 60. Undertreatment of older patients with acute myeloid leukemia (AML) can explain, in part, their inferior outcome when compared to that of younger patients. In agreement with the benefit seen by patients under age 60 from high-dose cytosine arabinos...