geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Selective amputation of the pharynx identifies a FoxA-dependent regeneration program in planaria


, : Selective amputation of the pharynx identifies a FoxA-dependent regeneration program in planaria. Elife 3: E02238-E02238

Planarian flatworms regenerate every organ after amputation. Adult pluripotent stem cells drive this ability, but how injury activates and directs stem cells into the appropriate lineages is unclear. Here we describe a single-organ regeneration assay in which ejection of the planarian pharynx is selectively induced by brief exposure of animals to sodium azide. To identify genes required for pharynx regeneration, we performed an RNAi screen of 356 genes upregulated after amputation, using successful feeding as a proxy for regeneration. We found that knockdown of 20 genes caused a wide range of regeneration phenotypes and that RNAi of the forkhead transcription factor FoxA, which is expressed in a subpopulation of stem cells, specifically inhibited regrowth of the pharynx. Selective amputation of the pharynx therefore permits the identification of genes required for organ-specific regeneration and suggests an ancient function for FoxA-dependent transcriptional programs in driving regeneration. DOI: http://dx.doi.org/10.7554/eLife.02238.001.

US$19.90

PMID: 24737865

DOI: 10.7554/eLife.02238


Other references

Harding, P.H., 1968: A strawberry cultivar trial at six centres (1965-67). The cultivars under trial were Regina, Gorella, Merton Herald, Senga Sengana, Templar, Crusader and Red-gauntlet. Details are given of the yields of marketable and large-grade fruit and the percentage of unmarketable fruit for each cultivar, with...

Ahsan, M.F.; Saeed, M.; Abu, 2004: Some aspects of breeding biology of the Bengal lizard (Varanus bengalensis) in Bangladesh. Some aspects of breeding biology of the Bengal, or Gray, Monitor Lizard (Varanus bengalensis) were studied in the farm area of Azra Produces Impex (a private enterprise) at Bhaluka, Mymensingh from 1995 to 1997. The feeding success, caring, egg-la...

Pieruccini Renzo, 1952: I sedimenti dellAppennino settentrionale toscano ed i problemi relativi al loro studio mineralogico, petrochimico e geochimico. Discussion of problems relating to the mineralogical and petrochemical study of sedimentary rocks of the northern Apennines of Tuscany, Italy.

Mojski Jozef Edward, 1970: The stratigraphy of the last glaciation in the territory of Poland. Pleistocene, pollen diagrams, lithofacies.

Hausdorf, B.; Riedel, A., 1996: Redescription of Vitrea sorella (Mousson 1863) from Turkey (Gastropoda: Pulmonata: Zonitoidea). Vitrea sorella (MOUSSON 1863) was described from a shore in Georgia. However, it has not been found again in Caucasia since the original description. Recently it was discovered in the Vilayets Zonguldak and Kastamonu in the Western Pontic mountain...

Martino, T.A.; Oudit, G.Y.; Herzenberg, A.M.; Tata, N.; Koletar, M.M.; Kabir, G.M.; Belsham, D.D.; Backx, P.H.; Ralph, M.R.; Sole, M.J., 2008: Circadian rhythm disorganization produces profound cardiovascular and renal disease in hamsters. Sleep deprivation, shift work, and jet lag all disrupt normal biological rhythms and have major impacts on health; however, circadian disorganization has never been shown as a causal risk factor in organ disease. We now demonstrate devastating ef...

Anderson, D.R.; Cooch, E.G.; Gutierrez, R.J.; Krebs, C.J.; Lindberg, M.S.; Pollock, K.H.; Ribic, C.A.; Shenk, T.M., 2003: Rigorous science: Suggestions on how to raise the bar. We offer suggestions for improving the validity of scientific inferences in wildlife ecology and management. These suggestions are offered under 7 categories as subjects of debate and refinement within our profession: university education, manuscr...

Higa, A.; Khandakar, J.; Mori, Y.; Kitamura, Y., 2013: Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency. Riboflavin secretion by Hyoscyamus albus hairy roots under Fe deficiency was examined to determine where riboflavin is produced and whether production occurs via an enhancement of riboflavin biosynthesis or a stimulation of flavin mononucleotide (...

Fry, C.H.; McGuigan, J.A., 1990: The influence of pH on Ca2+ exchange in ferret heart mitochondria. The effect of pH changes on Ca2+ transport by isolated heart mitochondria was measured. Two components of Ca2+ transport were identified, an accumulation dependent on mitochondrial respiration and a Na+-dependent efflux. A decrease of pH over the...

Abstracts., 1984: Abstracts of the 8th European Neuroscience Congress The Hague, the Netherlands September 11-15, 1984. Neuroscience Letters Supplement, S1-S458 No. 18