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Sensitive and selective detection of mycoplasma in cell culture samples using cantilever sensors


, : Sensitive and selective detection of mycoplasma in cell culture samples using cantilever sensors. Biotechnology and Bioengineering 105(6): 1069-1077

In this article we report a new biosensor-based method that is more sensitive and rapid than the current approach for detecting mycoplasma in cell culture samples. Piezoelectric-excited millimeter-sized cantilever (PEMC) sensors respond to mass change via resonant frequency change. They are sensitive at femtogram level and can be used directly in liquid for label-free detection. Common cell culture contaminant, Acholeplasma laidlawii was detected in both buffer and cell culture medium. Two different sources (positive control from a commercial kit and ATCC 23206) were analyzed using antibody-immobilized PEMC sensor. Resonant frequency decrease caused by binding of A. laidlawii was monitored in real-time using an impedance analyzer. Positive detection was confirmed by a second antibody binding. The limit of detection (LOD) was lower than 10(3) CFU/mL in cell culture medium using PEMC sensor while parallel ELISA assays showed LOD as 10(7) CFU/mL. This study shows that PEMC sensor can be used for sensitive and rapid mycoplasma detection in cell culture samples.

US$19.90

PMID: 20014143

DOI: 10.1002/bit.22637


Other references

Schlanderer, G.D.el, F.H.ld, W.D.llweg, H., 1975: A contribution to methanol metabolism and its regulation in yeasts. Symposium: Mikrobielle Proteingewinnung: obielle Proteingewinnung, 1st 25-33

Anonymous, 1973: Crop variety recommendations for 1974. Wheat, barley, oats, lupin, oilseed flax, rapeseed and field pea cultivars recommended for sowing in all the cropping zones of Western Australia are given.

Oku T.; Ohira Y., 1987: Variation in the hatching period of overwintering apple tortrix eggs archips fuscocupreana walsingham according to the deposition site in dwarf apple trees. The apple tortrix, Archips fuscocupreana Walsingham, deposits its overwintering egg masses on trunks and branches of the host trees. In the spring of 1985, eight drawf apple trees were selected and the crown was stratified every 50 cm from the gro...

Smith, MN., 2004: Hydroporus neglectus Schaum, 1845 (Coleoptera: Dytiscidae) new to Ireland. Irish Naturalists' Journal, 2710: 401-402

Ge, R-Shan.; Chen, G-Rong.; Tanrikut, C.; Hardy, M.P., 2007: Phthalate ester toxicity in Leydig cells: developmental timing and dosage considerations. Humans have significant exposures to phthalates, as these chemical plasticizers are ubiquitously present in flexible plastics. Recent epidemiological evidence indicates that boys born to women exposed to phthalates during pregnancy have an increa...

Muraro, R.; Roka, F.; Rouse, R., 1999: Budgeting costs and returns for southwest Florida citrus production, 1998-1999. Economic information report: 99 (99-5)

Alluisi, E.A.; Coates, G.D., 1967: A code transformation task that provides performance measures of nonverbal mediation (COTRAN). NASA Contract Rep NASA CR-895. Nasa Contractor Report. Nasa Cr. United States. National Aeronautics and Space Administration: 1-37

Anonymous, 2013: Non-Equilibirum ionisation and cooling of metal-enriched gas in the presence of a photo-ionisation background. Simulations of the formation of galaxies, as well as ionisation models usedto interpret observations of quasar absorption lines, generally either assumeionisation equilibrium or ignore the presence of the extra-galactic background(EGB) radiation....

Abrahamsen, D., 1955: Status of mental hygiene and child guidance facilities in public schools in the United States. Journal of Pediatrics 46(1): 107-118

Belfrage, A.; Grotmol, K.Støen.; Lien, L.; Moum, Tørn.; Wiese, R.Veslemøy.; Tyssen, R., 2017: Medical school predictors of later perceived mastery of clinical work among Norwegian doctors: a cohort study with 10-year and 20-year follow-up. Doctors' self-perceived mastery of clinical work might have an impact on their career and patient care, in addition to their own health and well-being. The aim of this study is to identify predictors at medical school of perceived mastery lat...