+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Setaria cervi dual specific phosphatase: characterization and its effect on eosinophil degranulation

, : Setaria cervi dual specific phosphatase: characterization and its effect on eosinophil degranulation. Parasitology 136(8): 895-904

Setaria cervi, a bovine filarial parasite contains significant acid phosphatase (AcP) activity in its various life stages. Two forms of AcP were separated from somatic extract of adult female parasite using cation exchange, gel filtration and concavalin affinity chromatography. One form having a molecular mass of 79 kDa was characterized as dual specific protein tyrosine phosphatase (ScDSP) based on substrate specificity and inhibition studies. With various substrates tested, it showed significant activity in the order of phospho-L-tyrosine>pNPP>ADP>phospho-L-serine. Inhibition by orthovanadate, fluoride, molybdate, and zinc ions further confirms protein tyrosine phosphatase nature of the enzyme. Km and Vmax determined with various substrates were found to be 16.66 mM, 25.0 microM/ml/min with pNPP; 20.0 mM, 40.0 microM/ml/min with phospho-L-tyrosine and 27.0 mM, 25.0 microM/ml/min with phospho-L-serine. KI with pNPP and sodium orthovanadate (IC50 33.0 microM) was calculated to be 50.0 mM. Inhibition with pHMB, silver nitrate, DEPC and EDAC suggested the presence of cysteine, histidine and carboxylate residues at its active site. Cross-reactivity with W. bancrofti-infected sera was demonstrated by Western blotting. ScDSP showed elevated levels of IgE in chronic filarial sera using ELISA. Under in vitro conditions, ScDSP resulted in increased effector function of human eosinophils when stimulated by IgG, which showed a further decrease with increasing enzyme concentration. Results presented here suggest that S. cervi DSP should be further studied to determine its role in pathogenesis and the persistence of filarial parasite.


PMID: 19523248

DOI: 10.1017/S0031182009006271

Other references

Rahman, H.; Hazarika, G.N.; Alam, S., 1996: Genetic variability and heterosis in a few crosses of chilli. Information on genetic variability, heritability and heterosis is derived from data on 9 yield-related characters in 4 Capsicum annuum varieties and their F1 hybrids from 6 crosses grown during kharif 1993 at Jorhat. High heritability was estimate...

Cui, J.; Hu, W.; Cai, Z.; Liu, Y.; Li, S.; Tao, W.; Xiang, H., 2010: New medicinal properties of mangostins: analgesic activity and pharmacological characterization of active ingredients from the fruit hull of Garcinia mangostana L. The fruit hull of <i>Garcinia mangostana</i> L. contains oxygenated and prenylated phenol derivatives, such as xanthones or xanthen-9H-ones, and is used by people in Southeast Asia as a traditional medicine for the treatment of abdomin...

Wolf, J., 1957: The relief picture of the epithelial conjunctiva with light and electron microscopy and its significance. In cross-sections of the conjunctiva of rabbits examined by the wet-mount method, one can differentiate the superficial squamous cells and the results of their secretion. They show a fine lobate structure. The surface of the fornix epithelium is e...

Delgado-Martinez, A.J.rasek, V., 1982: Extensity of infestation by most important nematodes of cattle in Cuba. II. Oesophagostomum sp. Folia veterinariaub 1982) 23(3-4): 233-245

Bandopadhyay Sukumar; Nelson Michael G., 1994: Multiple-criteria group decision support systems with application to a surface mine equipment selection problem. Many aspects of decision making and many decision problems in the mining engineering domain are not programmable. In dealing with these unstructured problems, engineering decisions are based on a combination of "objective" knowledge, &qu...

Sonin, M.D.; Soshkin, D.V.; Tseitlin, D.G., 1997: Eimeria nordensheldi sp n, a new species of coccidia from the lemming. While studying parasites of the lemmings Dicrostonyx torquatus vinogradovi Ognev, 1948 from the coast of the Vrangel Island a new species Eimeria nordensheldi was found out. Its description, figure and differential diagnosis are given.

Cidlinsky K.; Ibrmajer I., 1986: Contribution of seismic reflection to the knowledge of the tectonics of the Pilsen Basin and its basement. Vestnik Ustredniho Ustavu Geologickeho = Bulletin of the Geological Survey, Prague 61(1): 11-21

Th. Forster; M.E.E.ans; F.H.ller, 1994: The frequency dependence of low field susceptibility in loess sediments. Geophysical Journal International 118(3): 636-642

Bailey, R.; Schleiter, K.E., 2010: Testing Manufacturer Liability in FDA-Approved Device Malfunction. Virtual Mentor 12(10): 800-803

Freedman, L.Z., 1963: Forensic psychiatry. American Journal of Psychiatry 119: 669-675