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Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury


, : Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury. Chinese Medical Journal 123(10): 1320-1328

Studies suggested that anesthetics administered upon the early reperfusion or "anesthetic postconditioning" could protect post-ischemic hearts against myocardial ischemia reperfusion injury (MIRI). However, the mechanism responsible for such protection was not well-elucidated. We investigated the cardioprotection induced by sevoflurane postconditioning (SpostC) in rat hearts in vitro, and the respective role of phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated kinase 1 and 2 (ERK 1/2), mitochondrial K(ATP) channels (mitoK(ATP)) and mitochondrial permeability transition pore (mPTP), by selectively inhibiting PI3K, ERK 1/2, mitoK(ATP), with LY294002 (LY), PD98059 (PD), 5-hydroxydecanoate (5-HD) and by directly opening of mPTP with atractyloside (ATR), respectively. Isolated rat hearts were randomly assigned to one of the 12 groups (n = 15): Time control (continuous perfusion), ISCH (30 minutes of ischemia followed by 60 minutes of reperfusion alone), SpostC (3% sevoflurane postconditioning was administered during the first 15 minutes of reperfusion after 30 minutes of ischemia), ISCH + LY, ISCH + PD, ISCH + ATR, ISCH + 5-HD and ISCH + dimethyl sulfoxide (DMSO) groups (LY, PD, ATR, 5-HD and DMSO (the vehicle) was administered respectively during the first 15 minutes of reperfusion following test ischemia), SpostC + LY, SpostC + PD, SpostC + ATR and SpostC + 5-HD groups (LY, PD, ATR and 5-HD was coadministered with 3% sevoflurane, respectively). Hemodynamics was compared within and between groups. Infarction size was determined at the end of experiments using triphenyltetrazolium chloride (TTC) staining. Lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) and cardiac troponin I (cTnI) released from necrotic myocardium, were compared among TC, ISCH and SpostC groups. To investigate the relationships between RISK and mPTP implicated in SpostC, NAD(+) content in myocardium, a marker of mPTP opening, was compared among some experimental groups (TC, ISCH, ISCH + LY, ISCH + PD, ISCH + DMSO, SpostC, SpostC + LY, SpostC + PD). To further investigate whether the anti-apoptotic mechanism is implicated in SpostC-induced cardioprotection and its association with mitochondria, TUNEL staining was performed in some experimental groups (TC, ISCH, ISCH + 5-HD, ISCH + ATR, ISCH + DMSO, SpostC, SpostC + 5-HD, SpostC + ATR). When compared with unprotected hearts subjected to 30 minutes of ischemia, exposure to 3% sevoflurane for 15 minutes during early reperfusion significantly improved functional recovery, decreased myocardial infarct size, decreased LDH, CK-MB and cTnI release, and decreased cardiomyocyte apoptosis (P < 0.05). However, such cardioprotective effects of hemodynamic recovery and infarct size reduction by sevoflurane was completely abolished by any one of LY294002, PD98059, atractyloside and 5-hydroxydecanoate (P < 0.05). Additionally, either LY294002 or PD98059 could reverse the inhibitory effect of SpostC over mPTP opening upon reperfusion (P < 0.05). Both atractyloside and 5-hydroxydecanoate could abrogate the anti-apoptotic effects of SpostC (P < 0.05). These findings demonstrate that PI3K, ERK 1/2, mitoK(ATP) and mPTP are key players in sevoflurane postconditioning induced cardioprotective mechanisms in isolated rat hearts subjected to MIRI.

US$29.90

PMID: 20529589


Other references

Fernicola, A.R., 1970: Primary paravesical hemangioma. Journal of the Medical Society of New Jersey 67(9): 523-526

Ham, C.; Akinnifesi, F.K.; Franzel, S.; Jordaan, D. du P.S.; Hansmann, C.; Ajayi, O.C.; Kock, C. de, 2007: Opportunities for commercialization and enterprise development of indigenous fruits in Southern Africa. This chapter discusses the marketing of fruit trees and fruits in Southern Africa. Marketability, market prices, indigenous fruit processing, and postharvest transport and handling are explained in detail.

Denden, S.; Bouden, B.; Haj Khelil, A.; Ben Chibani, J.; Hamdaoui, M.H., 2016: Gender and ethnicity modify the association between the CYP1A2 rs762551 polymorphism and habitual coffee intake: evidence from a meta-analysis. The association between the single nucleotide polymorphism rs762551 in the cytochrome P450 family 1, subfamily A2 gene (CYP1A2) and caffeine consumption remains controversial. We conducted a meta-analysis to clarify this potential association. Twe...

Argov, N.; Sklan, D.; Zeron, Y.; Roth, Z., 2006: Association between seasonal changes in fatty-acid composition, expression of VLDL receptor and bovine sperm quality. Reduced reproductive performance of dairy cows during the summer is often associated with elevated temperature. Semen collected and cryopreserved during the summer may be of low quality and might contribute to the compromised fertility of dairy co...

Anonymous, 2012: Cancer data in the cloud could lead to more effective treatment. Researchers are using cloud technology to collect information from thousands of cancer cell samples. The goal: better predictions about how a patient's illness will progress and what type of treatment will be most effective.

Dejgaard, A.; Larsen, J.; Pedersen, C.Ross., 2007: Type I and type IV allergy to insulin detemir. Lancet 369(9577): 1926; Author Reply 1926-7

Szweykowski, J.Z.elinski, R., 1983: Isoenzymatic variation in Polish populations of the moss Plagiothecium undulatum (Hedw.) B.S.G. A preliminary report. Journal of the Hattori Botanical Laboratory: (54) 119-123

Vasilenko, F.I.; Grigor'eva, I.G., 1987: Early and late acute recurrences of tick-borne encephalitis. The article analyzes 135 cases of tick-borne encephalitis (TBE) treated at a neurological hospital over the last 20 years. Inapparent form of TBE was diagnosed in 3.7% of cases, febrile in 48.3%, meningeal in 36.3%, focal in 11.7%. Attention is dr...

Musumeci, D.; Bucci, E.M.; Roviello, G.N.; Sapio, R.; Valente, M.; Moccia, M.; Bianchi, M.E.; Pedone, C., 2011: DNA-based strategies for blocking HMGB1 cytokine activity: design, synthesis and preliminary in vitro/in vivo assays of DNA and DNA-like duplexes. In this work we report the design and synthesis of kinked oligonucleotide duplexes as potential inhibitors of HMGB1, a cytokine which triggers a broad range of immunological effects. We found that the designed ligands can interact with HMGB1, as e...

Patra K.C.; Bhunia A.B.; Panigrahy R.C.; Mitra A., 1990: Seasonal variations of primary production of phytoplankton in a tidal creek in the midnapore district of west bengal india. Manna, G K And B B Jana (Ed ) Impacts Of Environment on Animals And Aquaculture; National Symposium, Kalyani, West Bengal, India, May 14-16, 1988 Xxiii+333p Kalyani University Fisheries And Limnology Research Unit: Kalyani, West Bengal, India Illus Maps : 177-182