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Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model


, : Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model. Acta Cirurgica Brasileira 28(4): 282-287

To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18 ± 0.1 for the total cohort; 0.14 ± 0.06 for the 4h reperfusion groups and 0.19 ± 0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.

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PMID: 23568236


Other references

Holm, M.V.; Szucs, T.D.; Fara, G.M., 2008: Developments in influenza vaccination coverage in Italy over five seasons (2001-2006). This study aims at assessing trends in influenza vaccination coverage from 2001 to 2006 in Italy, at understanding the drivers and barriers to vaccination, and to identify vaccination intentions for season 2006/07. We conducted telephone-based hou...

Nir, Z.; Neumann, L., 1991: Self-esteem, internal-external locus of control, and their relationship to weight reduction. One hundred sixteen women participated in a 10-week weight reduction program and lost on the average of 7 kg. Family-related variables and two personality constructs, self-esteem and Internal-External Locus of Control (I-E Loc), were examined as p...

Kierych E., 1975: Data on the aphidiidae hymenoptera of poland. Fragmenta Faunistica (warsaw): 233-246

Panissod C.; Camerlynck C., 1996: Subsurface imagery combining the use of radar and electrical methods. Reunion Annuelle des Sciences de la Terre 16: 188

Haj, A., 1996: The role of irrigation in Morocco: sustainable water management. Morocco has a wide programme for the development of irrigation, but now other sectors such as municipal and industrial are competing for available water. Studies have shown that:- (i) the rainfed agricultural sector is far from achieving national...

Andervont, H.B., 1957: Immunology & cancer: summary. Annals of the New York Academy of Sciences 69(4): 848-855

Raaijmakers, W.; Prickaerts, J.; Schoffelmeer, A.; Jolles, J., 1994: The novel cognition enhancing agent S 12024-2 facilitates object and social recognition memory after acute or chronic treatment in rats. Society for Neuroscience Abstracts 20(1-2): 1765

Roman, M.A., 1983: The effect of the acaricide Milbol EC (dicofol 18.5%) on the mite fauna attacking orange. Field trials were carried out in Cuba to determine the effects of dicofol (Milbol) at 2% on the mite fauna in an orange grove. The main phytophagous species in the grove were Phyllocoptruta oleivora (Ashm.), Tetranychus urticae Koch, Eutetranychus...

Bronisz, A.; Rosc, D.; Bronisz, M.; Szymanski, W.; Junik, R., 2008: Coagulation and fibrinolysis variables in pregnant women with type 1 diabetes mellitus. Many studies have reported changes in the hemostatic system in patients with type 1 diabetes in whom coagulation processes predominate over fibrinolytic activity. The aim of this study was to assess some of the hemostatic variables during pregnanc...

Moses R.L.; Delcarpio J.B.; Boogaerts J., 1982: Neo natal cardiac myocytes cultured on reconstituted collagen gels. Journal of Molecular & Cellular Cardiology 14(SUPPL 1): 52