+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Simulating tumor microenvironment: changes in protein expression in an in vitro co-culture system

, : Simulating tumor microenvironment: changes in protein expression in an in vitro co-culture system. Cancer Cell International 14(): 40-40

The role of the microenvironment during the initiation and progression of carcinogenesis is thought to be of critical importance, both for the enhanced understanding of fundamental cancer biology as well as for improving molecular diagnostics and therapeutics. The aim of this study was to establish an in vitro model based on a co-culture of healthy human fibroblasts (HFs) and human osteosarcoma cells (MG-63s) to simulate the microenvironment including tumor and healthy cells. The HFs and MG-63s were in vitro co-cultured for a period of time ranging from 24 h to 7 days. Cell morphology and organization were studied using phase contrast microscopy while the expression of Human Cartilage Glycoprotein 39 (YKL-40), Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloprotease 1 (MMP1) was investigated by Real Time PCR and Western Blotting. The results showed a characteristic disposition of tumor and healthy co-cultured cells in columns which are not visible in tumor and healthy cells grown singularly. The expression of YKL-40, VEGF and MMP1 significantly changed in co-cultured cells compared to HFs and MG-63s separately cultured. We concluded that the tumor microenvironment has an influence on the protein expression of the healthy surrounding tissues and the process of tumorigenicity.


PMID: 24883044

DOI: 10.1186/1475-2867-14-40

Other references

Gonzalez Adrian; Graves Duane; Kearney Theresa; Holroyd Chris; Eccles Harry, 1997: Demonstration of an innovative heavy metal and radionuclide bioremediation process. Heavy metals contamination has historically been considered outside the realm of environmental issues suitable for treatment by biological processes. In recent years, growing interest in metals bioremediation has resulted in a few techniques that...

Rainwater E.H., 1964: Regional stratigraphy of the Midway and Wilcox in Mississippi. Alternating beds of sand, silt, and shale comprise the Midway and Wilcox section in Mississippi; thickness varies from 500 to 5,000 feet. Late Paleocene and early Eocene were times of major regression with occasional transgressions. Deltaic enviro...

Nehrash, A.K., 1961: The antimicrobial properties of cultivated Radish. Report II. The antimicrobial properties of a group of acid substances from the root of Black Radish. This complex killed Corynebacterium michiganense at 1: 50, 000-100, 000, C. sepedonicum and G. insidiosum (1: 25, 000-50, 000), Pseudomonas tabaci and P. syringae (1: 2, 500), Xanthomonas malvacearum (1: 10, 000), and X. beticola and B carotovorus...

Vallance John, 1911: The Standard Mine, Silverton, B. C. Transactions of the Canadian Mining Institute Pages 55-58

Ozaki, M.; Nagatomo, T.; Maeda, T.; Kishioka, S.; Yamamoto, H., 2006: Pharmacological differences between Liu-Jun-Zi-Tang, a traditional Chinese herbal medicine, and domperidone on isolated guinea-pig ileum. The different characteristics of Liu-Jun-Zi-Tang, a spray-dried powdered extract from 8 Chinese herbs, and domperidone, a D2 antagonist, both of which are used clinically for the treatment of gastritis, were validated in isolated guinea pig ileal...

Anonymous, 1988: NTP Toxicology and Carcinogenesis Studies of 1,2-Epoxybutane (CAS No. 106-88-7) in F344/N Rats and B6C3F1 Mice (Inhalation Studies). 1,2-Epoxybutane was selected for study because it is a short-chain epoxide that had been shown to be mutagenic and because no carcinogenicity data were available. Approximately 8 million pounds of 1,2-epoxybutane are produced annually in the Unite...

Wright J.E.; Howard K.A.; Anderson J.L., 1987: Isotopic systematics of zircons from Late Cretaceous intrusive rocks, southeastern California; implications for a vertically stratified crustal column. Abstracts with Programs - Geological Society of America 19(7): 898

Sacca, M., 1956: Simultaneous administration of penicillin, streptomycin and chloramphenicol in severe inflammatory lung diseases in children. Minerva Pediatrica 8(29-30): 944-945

Valencia, A.M.; Beharry, K.D.; Ang, J.G.; Devarajan, K.; Van Woerkom, R.; Abrantes, M.; Nishihara, K.; Chang, E.; Waltzman, J.; Modanlou, H.D., 2003: Early postnatal dexamethasone influences matrix metalloproteinase-2 and -9, and their tissue inhibitors in the developing rat lung. In order to test the hypothesis that early postnatal exposure to dexamethasone (Dex) influences matrix metalloproteinases (MMP)-2 and -9, as well as their tissue inhibitors (TIMP-1 and -2) in the developing rat lung, newborn rats (3 litters/group)...

Stacher, A.; Stöckl, W., 1968: Modifications of the free amino acids in the plasma and in the erythrocytes of patients with pernicious anemia through vitamin B 12 therapy. Folia Haematologica 90(2): 269-274