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Simultaneous determination of triterpenoid saponins in dog plasma by a validated UPLC-MS/MS and its application to a pharmacokinetic study after administration of total saponin of licorice


, : Simultaneous determination of triterpenoid saponins in dog plasma by a validated UPLC-MS/MS and its application to a pharmacokinetic study after administration of total saponin of licorice. Journal of Pharmaceutical and Biomedical Analysis 75: 248-255

A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents, uralsaponin C, uralsaponin F, 22β-acetoxyl-glycyrrhizin, 24-hydroxy-licorice-saponin E2, licorice-saponin G2, licorice-saponin E2, glycyrrhizin, and licorice-saponin J2, from total saponin of licorice (TSL) in dog plasma. Ardisiacrispin A was used as the internal standard (IS). The separation was performed on Thermo Syncronic C₁₈ column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 mL min⁻¹, and acetonitrile/methanol (3:1, v/v)-0.1% formic acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with negative ionization mode. All calibration curves had good linearity (r>0.991) over the concentration range from 2.03-405 ng mL⁻¹ to 2.63-2625 ng mL⁻¹ for all components. The intra- and inter-day precision was within 15% and the accuracy ranged from -14.08% to 13.8%. The method was successfully applied to pharmacokinetic study of eight triterpenoid saponins in dog plasma after oral administration of TSL.

US$19.90

PMID: 23280090

DOI: 10.1016/j.jpba.2012.11.035


Other references

Daoudi, F.; Marques, A., 1987: Eimeria bouixi n. sp. et Eimeria dicentrarchi n. sp. (Sporozoa-Apicomplexa). Coccidies parasites du loup Dicentrarchus labrax (Linne, 1758), en region languedocienne. Annales des Sciences Naturelles Zoologie et Biologie Animale. 1986- ; 84: 237-242

Laux L.F.; Lane D.M., 1985: Information processing components of substitution test performance. Although substitution tests have been included in tests of intelligance for years, the underlying abilities they measure have still not been clearly determined. Componential analysis was used to investigate the information-processing components un...

Berera; Dawson, 1991: Sine-Gordon renormalization-group analysis of the orientational preroughening transition on a triangular lattice. Physical Review. A, Atomic, Molecular, and Optical Physics 44(6): 3466-3470

Zulli F.; Gysler C.; Rey Berthod C.; Niederberger P., 1990: Determination of the ploidy of commercial bakers yeast strains. Yeast 6(SPEC ISSUE): S598

Sato, H.; Wada, Y.; Itabashi, T.; Nakamura, M.; Kawamura, M.; Tamai, M., 2005: Mutations in the pre-mRNA splicing gene, PRPF31, in Japanese families with autosomal dominant retinitis pigmentosa. PURPOSE: To describe the clinical and genetic characteristics of three Japanese families with autosomal dominant retinitis pigmentosa (ADRP) associated with mutations in the PRPF31 gene.DESIGN: Case reports and results of DNA analysis.METHODS: Mut...

Schacter, B.A.; Mason, J.I., 1974: The effect of phenobarbital, 3-methylcholanthrene, 3,4-benzpyrene, and pregnenolone-16 alpha-carbonitrile on microsomal heme oxygenase and splenic cytochrome P-450. Archives of Biochemistry and Biophysics 160(1): 274-278

Mayer, R., 1967: Breeding of vegetables at the National Institute of Agronomic Research, France. Of the French bean varieties tested for reaction to six races of Colletotrichum lindemuthianum the only resistant ones were some Mexican lines and Cornell 49242, the resistance gene in at least one of the Mexican lines being different front those...

Kiraly, K.; Horvath, I., 1976: Survival of treponema pallidum under micro aerobic conditions in cell and tissue cultures. The survival of pathogenic Treponema pallidum was studied under microaerobic conditions (partial pressure of O2 7 mm Hg) with and without tissue cell cultures. As 1/3-1/2 of the germs were motile even after 96 h incubation, treponemes do not seem...

Hughes, J.R., 1985: Natural history of hypsarhythmia. Clinical Eeg 16(3): 128-130

Kästner, I., 1990: Reactions of German medical intelligentsia to World War I. Starting from the ideological position of physicians before the first world war it is shown that the majority of physicians accepted this war and that pacifists among them belonged to a minority, which, however, relentlessly unmasked the culture-d...