+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Simultaneously optical recording of membrane potential in population vestibular ganglion neurons

, : Simultaneously optical recording of membrane potential in population vestibular ganglion neurons. Zhongguo Ying Yong Sheng Li Xue Za Zhi 18(3): 306-309

To investigate membrane electro-physiological features in vestibular ganglion neuronal population using a voltage-sensitive dye and optical recording technique. Dissociated and cultured mouse vestibular ganglion neurons were stained with an absorption voltage-sensitive dye, RH 155, and were imaged in 16 x 16 elements Photodiode arrays (PDA) optical recording system. When the cells were depolarized during perfusion with 150 mmol/L potassium solution, optical absorption of the dye that bound to the external surface of neuron membranes increased. The relative ratio (delta I/I) of optical absorption change was 0.23% +/- 0.08% (x +/- s, n = 28). These optical responses were wavelength dependent. Under our experimental conditions, photo toxicity and pharmacological effects of the dye were either absent or insignificant. Our results suggest that optical recording provides a new, practical and less toxic method to simultaneously monitor changes in membrane potential from several cultured vestibular ganglion cells.


PMID: 21180079

Other references

Galvani, A.P., 2003: Immunity, antigenic heterogeneity, and aggregation of helminth parasites. Empirical studies of helminth parasites reveal that the distribution of parasite burdens in their host populations is highly aggregated. This aggregation is fundamental to the ecology and epidemiology of helminth parasites. Results from a stochast...

Wetzstein R.; Renn K.H., 1970: Studies on the structure of human myometrium. Verhandlungen der Anatomischen Gesellschaft 64: 599-600

Parkin; Engler; Lee; Nazzal; Tokura; Torrance; Grant, 1988: Magnetic field dependence of the resistivity and susceptibility of the above-100-K Bi-Sr-Ca-Cu superconductor. Physical Review. B, Condensed Matter 38(10): 7101-7104

Yang-Jun; Li-Jun; He-Shenxu, 2001: Determination of gastrointestinal contents' acoustical quality and the application in HIFU therapy for tumors in abdomen. Objective: To research the acoustical quality of gastrointestinal contents and the attenuation criterion of ultrasound passing through gastrointestinal contents. Methods: The velocity, impedance, attenuation coefficient of sound were calculated by...

Kimmig, J.; Meyer, R.Hn.J., 1962: Clinical and experimental studies on the chemotherapeutic effectiveness of sulfadiazines substituted in position 5 and the combination of 5-methylsulfadiazine with a derivative of penicillin. Medizinische Klinik 57: 1437-1442

Berglund, A.; Rosenqvist, G.; Svensson, I., 1986: Reversed sex roles and parental energy investment in zygotes of two pipefish Syngnathidae species. In pipefishes, males brood their offspring on their body or in a brood pouch. In 2 species of pipefish, Siphonostoma typhle and Nerophis ophidion , sexual dimorphism, dichromatism, and sex role reversal differed in degree between the species. The...

Jacobs, S.J., 1989: Micro-organisms as potential biological control agents of Eldana saccharina Walker (Lepidoptera: Pyralidae). The bacterium Bacillus thuringiensis produces a toxic crystal which is lethal to Lepidoptera. Local isolates from the sugarcane pest Eldana saccharina in South Africa caused higher mortalities to the pyralid in the laboratory than did Thuricide (R...

Abdelkarim, H.; Brunsteiner, M.; Neelarapu, R.; Bai, H.; Madriaga, A.; van Breemen, R.B.; Blond, S.Y.; Gaponenko, V.; Petukhov, P.A., 2015: Photoreactive "nanorulers" detect a novel conformation of full length HDAC3-SMRT complex in solution. Histone deacetylase 3 (HDAC3) is a promising epigenetic drug target for multiple therapeutic applications. Direct interaction between the Deacetylase Activating Domain of the silencing mediator for retinoid or thyroid-hormone receptors (SMRT-DAD)...

Bonadio, C.M.; Feldman, E.C.; Cohen, T.A.; Kass, P.H., 2015: Comparison of adrenocorticotropic hormone stimulation test results started 2 versus 4 hours after trilostane administration in dogs with naturally occurring hyperadrenocorticism. Trilostane medical treatment of naturally occurring hyperadrenocorticism (NOH) in dogs is common, as is use of the adrenocorticotropic hormone (ACTH) stimulation test (ACTHst) in monitoring response to treatment. There is uncertainty regarding whe...

Tang, W.W.; Feng, L.; Vannice, J.L.; Wilson, C.B., 1992: IL-1 receptor antagonist ameliorates anti-GBM antibody associated glomerulonephritis. Journal of the American Society of Nephrology 3(3): 618