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Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

, : Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides. Optics Express 16(14): 10077-10090

Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to approximately 17 mm/s. We derive an analytic scaling relationship [equation: see text] converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.


PMID: 18607415

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