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Site-specific two-color protein labeling for FRET studies using split inteins

, : Site-specific two-color protein labeling for FRET studies using split inteins. Journal of the American Chemical Society 131(33): 11644-5

Fluorescence resonance energy transfer (FRET) is an ideal tool for studying protein conformational changes and dynamics. Strategies to specifically label proteins with multiple colors at structurally important sites, a prerequisite for such studies, however, need to be devised on a protein-by-protein basis. Here we describe a simple yet widely applicable two-color site-specific protein labeling method utilizing Npu DnaE split intein.


PMID: 19645470

DOI: 10.1021/ja9030215

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