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Special stain and X-ray probe microanalysis of livers with Wilson disease


, : Special stain and X-ray probe microanalysis of livers with Wilson disease. Hepatology Research 39(6): 563-568

Primary copper toxicosis due to Wilson disease is clinically complex, often leading to delayed diagnosis. Because the metabolic disorder is frequently complicated by iron overload due to hypoceruloplasminemia, either a special stain or microanalysis has been recommended for liver biopsy specimens. Liver biopsy was performed in three patients in whom Wilson disease was highly suspected. Light microscopic study included rubeanic acid stain for copper and Berlin blue stain for iron. To improve the resolution of ultra-structures and preservation of toxic metals, short-term fixation with a 0.1% osmic acid solution was applied for X-ray probe microanalysis. Their diagnosis was confirmed by genetic study and copper chelation therapy. Two patients at the age of 17 and 23 years, respectively, demonstrated cirrhotic livers surrounded by fibrous septa, while a 7-year-old patient demonstrated fatty liver with mildly expanded portal tracts. Both copper grains stained with rubeanic acid and cuprothionein by microanalysis were found in the cirrhotic livers of aged patients. However, either morphological method failed to detect copper deposition in fatty liver tissues from the young patient. Iron deposits were also found in the cirrhotic livers of aged patients. The molecular basis of Wilson disease was confirmed by gene analysis. All patients responded to copper chelation therapy. A morphological method of special staining or microanalysis improved with a new fixative may be unreliable for detecting diffusely distributed copper in the early stage of Wilson liver disease.

US$19.90

PMID: 19254344

DOI: 10.1111/j.1872-034X.2009.00491.x


Other references

McLeod, J., 1978: Quiet night. Nursing Times 74(38): 1576-1576

Coren S.; Girgus J.S., 1972: Illusion decrement in intersecting line figures. Psychonomic Science Section on Human Experimental Psychology 26(2): 108-110

Limb, M., 2016: Scientists take first steps to form united front over interactions with global food industry. Bmj 351: H6859-H6859

Vogelmann, Hw, 1982: Catastrophe on Camels Hump Acid rain is killing trees in many forests of North America and Europe. Natural history 91(11): 8

Blanksby, B.A.; Blanksby, J.P., 1995: The development of an aquatics program in an academic environment. The article reports on the development of a swimming programme at the University of Western Australia, which has been used to provide professional and academic microteaching, coaching and research opportunities for its student majors. Accreditatio...

Glaeser R.M., 1985: Structural changes in bacteriorhodopsin during the m state are limited in number and confined to high resolution. Biophysical Journal 47(2 PART 2): 322A

Osteras, O., 1982: Results from a practical mastitis control programme. A mastitis control programme was carried out on 158 Norwegian herds, known to have a high somatic cell count in their milk, over the 2 yr 1978-1979; av. size of herd was 11 cows. The programme included recording of milk somatic cell count, examina...

Casarotti, H., 2012: The evolution of psychiatry in Uruguay. Uruguay, a country in South America situated between Brazil and Argentina, has a population of 3 million, a literacy rate of 97%, and health coverage of 87% . Treatment of patients with mental disturbances has existed throughout its history. From...

Bouillon, R., 2002: The future of endocrinology and the endocrinologist of the future. Hormone Research 56 Suppl 1: 98-105

Caputo M.V., 1972: The Paleozoic sedimentary section of Rio Tapajos. Boletim da Sociedade Brasileira de Geologia 3): 29-34