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Spectrofluorimetric determination of SN-38, a promising new anti-tumor agent, in the presence and absence of organized media

, : Spectrofluorimetric determination of SN-38, a promising new anti-tumor agent, in the presence and absence of organized media. Applied Spectroscopy 65(3): 298-306

This study focuses on the spectrofluorimetric behavior of the camptothecin derivative 7-ethyl-10-hydroxycamptothecin (SN-38) alone and in the presence of organized media and also on its potential analytical applications. SN-38 displays native fluorescence in both lactone and carboxylate form, which has been the base for development of two spectrofluorimetric methods, one for the lactone form (acidic media) and another for the carboxylate form (basic media). In an attempt to improve the understanding of SN-38, its interaction with several cyclodextrins and surfactants has been studied using spectrofluorimetry. Consequently, the optimal working conditions for the determination of SN-38 have been established in both the presence and the absence of organized media. The proposed methods were applied to human urine, using liquid-liquid extraction for clean-up of the samples, with satisfactory recoveries. No interference of the urine matrix was observed.


PMID: 21352650

DOI: 10.1366/10-06019

Other references

Kerns, W.L.; Glanz, M.P., 1989: Private social welfare expenditures, 1972-87. Social Security Bulletin 52(11): 18-26

Smit, B., 1926: Sheep Blow-fly Control. Fly-traps : Their Construction and Operation. The efficiency of the trap previously described by Wahl and Du Plessis has been increased by the addition of gauze windows in the sides of the upper tin similar to that in the top. Further structural improvements made by the author include the use...

Bunout, D.; Barrera, G.; Maza, P. de la; Gattas, V.; Hirsch, S., 2003: Seasonal variation in insulin sensitivity in healthy elderly people. OBJECTIVE: There is a seasonal variation in the incidence of diabetes mellitus and cardiovascular diseases. However, there is very little information about the seasonal variation in insulin sensitivity. We report the seasonal variation in insulin...

Wang, X.Ge.; Ju, Z.Hua.; Hou, M.Hai.; Jiang, Q.; Yang, C.Hong.; Zhang, Y.; Sun, Y.; Li, R.Ling.; Wang, C.Fa.; Zhong, J.Feng.; Huang, J.Ming., 2016: Correction: Deciphering Transcriptome and Complex Alternative Splicing Transcripts in Mammary Gland Tissues from Cows Naturally Infected with Staphylococcus aureus Mastitis. NlmCategory="UNASSIGNED">[This corrects the article DOI: 10.1371/journal.pone.0159719.].

Thomson W.J.; Gaunt R.E., 1986: The effect of speckled leaf blotch on apical development and yield in winter wheat in new zealand. Speckled leaf blotch (Mycosphaerella graminicola) reduced yield in a winter wheat trial by 18 per cent. Detailed analyses of the effects of disease on yield components showed that disease reduced both grain number per ear and grain weight. The red...

Borders, N.; Lawton, R.; Martin, S.R., 2012: A clinical audit of the number of vaginal examinations in labor: a NOVEL idea. Clinical practice audits present an opportunity for providers to examine and reflect on their practice. Vaginal examinations are an integral part of intrapartum care but can be uncomfortable for women and can increase the risk of chorioamnionitis....

Howard T.M., 1973: Studies in the ecology of nothofagus cunninghamii part 1 natural regeneration on the mt donna buang massif victoria australia. Australian Journal of Botany 21(1): 67-78

Calapaj, G.G.; Ongaro, D., 1967: Research on the food use of radiocontaminated milk. The paper deals with the possibility of human consumption of the milk eventually radiocontaminated by a nuclear accident. The following points seem to be ascertained: in buttermaking the Sr90 and Cs137 levels in the commercial product do not excee...

Penzias, A.S., 2000: Infertility. Contemporary office-based evaluation and treatment. Infertility is a common condition that is highly treatable. Couples can help themselves by recognizing that they have a problem, and practitioners can help patients by recognizing who requires an infertility investigation and when they need it. In...

de Felipe, K.Suwwan.; Pampou, S.; Jovanovic, O.S.; Pericone, C.D.; Ye, S.F.; Kalachikov, S.; Shuman, H.A., 2005: Evidence for acquisition of Legionella type IV secretion substrates via interdomain horizontal gene transfer. Intracellular pathogens exploit host cell functions to create a replication niche inside eukaryotic cells. The causative agent of Legionnaires' disease, the gamma-proteobacterium Legionella pneumophila, resides and replicates within a modifie...