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Sperm protamine mRNA ratio and DNA fragmentation index represent reliable clinical biomarkers for men with varicocele after microsurgical varicocele ligation

, : Sperm protamine mRNA ratio and DNA fragmentation index represent reliable clinical biomarkers for men with varicocele after microsurgical varicocele ligation. Journal of Urology 192(1): 170-176

We investigated whether the sperm protamine-1/2 mRNA ratio and DNA fragmentation index are reliable biomarkers in patients with clinical varicocele. We performed a prospective study in 42 subfertile patients with left clinical varicocele and 10 normozoospermic healthy donors with proven fertility. All patients and female partners were seen 3 and 6 months after varicocelectomy for fertility examination. Real-time quantitative reverse transcriptase-polymerase chain reaction and SCSA® were performed to analyze the sperm protamine-1/2 mRNA ratio and DNA fragmentation index. The protamine-1/2 mRNA ratio and DNA fragmentation index were significantly higher in the preoperative group than in the control group (p <0.01). After varicocelectomy in the pregnant group the protamine-1/2 mRNA ratio recovered to the normal value and the DNA fragmentation index was significantly lower than in the preoperative group (p <0.01). In the nonpregnant group the protamine-1/2 mRNA ratio and DNA fragmentation index did not differ (p >0.05). However, significant differences were present preoperatively according to varicocele severity in the protamine-1/2 mRNA ratio and DNA fragmentation index (p <0.05 and <0.01, respectively) with the highest values in the grade 3 group. Nevertheless, postoperatively no difference in varicocele severity was noted (p >0.05). The protamine-1/2 mRNA ratio was strongly related to preoperative and postoperative sperm concentration (Rs -0.238, p <0.01), progressive motility (Rs -0.327, p <0.01), total motility (Rs -0.206, p <0.05) and DNA fragmentation index (Rs 0.293, p <0.01) except for normal morphology (Rs -0.064, p >0.05). The sperm protamine-1/2 mRNA ratio and DNA fragmentation index can effectively be used to evaluate male fertility. Male infertility due to varicocele may be associated with protamine deficiency and sperm DNA damage. The post-varicocelectomy protamine-1/2 mRNA ratio and DNA fragmentation index are associated with the post-varicocelectomy pregnancy rate.


PMID: 24582535

DOI: 10.1016/j.juro.2014.02.046

Other references

Hartmann, M., 1926: Uber experi-mentelle Unsterblichkeit von Protozoen-Individuen. Naturwissenschaften Berlin, 14: 433-435

Ube, T.; Minagawa, K.; Ikeda, T., 2017: Interpenetrating polymer networks of liquid-crystalline azobenzene polymers and poly(dimethylsiloxane) as photomobile materials. We developed photomobile polymer materials with interpenetrating polymer network (IPN) structures composed of crosslinked liquid-crystalline azobenzene polymer (PAzo) and poly(dimethylsiloxane) (PDMS). By introducing PDMS into a PAzo template netw...

Eleiwa, Mona, M.E., 2004: Effect of different concentrations of zinc. or cadmium on Vigna sinensis plants in presence or absence of arbuscular mycorrhizal fungi and rhizobia. yTHE PRESENT study, effect of Arbuscular mycorrhizal fungi and rhizobia in presence of different concentrations of zinc and -cadmium on growth and some metabolic activities of Vigna sinensis plant. was carried out. Also the effect of both heavy m...

Desalegn, G.; Binner, E.; Lechner, P., 2008: Humification and degradability evaluation during composting of horse manure and biowaste. Performing compost quality assessment such as compost stability is quite necessary for rating the quality of horse manure and biowaste composts and meeting specific regulatory requirements on the composition and compost process. The aim of this s...

Mindermann, T.; Wilson, C.B., 1995: Pediatric pituitary adenomas. Previous series of pediatric pituitary adenomas have been small and have not been analyzed by age group. We analyzed the frequency, manifestation, course, and biology of these tumors before, during, and after puberty in 136 children younger than 2...

Shimotsuma, M., 1965: A survey of seedless watermelon breeding and its extension in Taiwan. The main aim of breeding work taking place in Taiwan is to develop triploid hybrids which have a high yielding capacity, strong disease resistance and good table and transporting quality. Two introduced American diploid varieties, Sugar Baby and I...

Babcock, D.F.; Morris, R.O., 1973: Specific degradation of a plant leucyl transfer ribonucleic Acid by a factor in the homologous synthetase preparation. Partially purified aminoacyl synthetase preparations from pea roots (Pisum sativum L. var. Alaska) contain a heat-labile factor which can degrade leucyl-tRNA(6) (leu) to a new species. The singular electrophoretic and chromatographic mobilities, t...

Porfireva, NA., 1977: On the history of study of planarians in the University of Kazan. Unknown

Ishida, S.; Nihei, T.; Kawakami, M., 1977: Effects of ovulation blocking agents upon the limbic hypothalamic neuronal pathways in the rabbit brain. Female adult rabbits with coaxial bipolar electrodes chronically implanted in the brain were used. Evoked potentials (EVP) were recorded in the medial basal hypothalamus (MBH) including the median eminence and the arcuate nucleus in response to st...

Sampaio, M.; Sampaio, A.; Ferreira, N., 1954: Procaine penicillin G in aluminum monostearate in therapy of recent syphilis. Jornal Da Sociedade das Ciencias Medicas de Lisboa 118(4): 171-202