+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Stable isotope signatures confirm carbon and nitrogen gain through ectomycorrhizas in the ghost orchid Epipogium aphyllum Swartz

, : Stable isotope signatures confirm carbon and nitrogen gain through ectomycorrhizas in the ghost orchid Epipogium aphyllum Swartz. Plant Biology 13(2): 270-275

Epipogium aphyllum is a rare Eurasian achlorophyllous forest orchid known to associate with fungi that form ectomycorrhizas, while closely related orchids of warm humid climates depend on wood- or litter-decomposer fungi. We conducted (13) C and (15) N stable isotope natural abundance analyses to identify the organic nutrient source of E. aphyllum from Central Norway. These data for orchid shoot tissues, in comparison to accompanying autotrophic plants, document C and N flow from ectomycorrhizal fungi to the orchid. DNA data from fungal pelotons in the orchid root cortex confirm the presence of Inocybe and Hebeloma, which are both fungi that form ectomycorrhizas. The enrichment factors for (13) C and (15) N of E. aphyllum are used to calculate a new overall average enrichment factor for mycoheterotrophic plants living in association with ectomycorrhizal fungi (ε(13) C ± 1 SD of 7.2 ± 1.6 ‰ and ε(15) N ± 1 SD of 12.8 ± 3.9 ‰). These can be used to estimate the fungal contribution to organic nutrient uptake by partially mycoheterotrophic plants where fully mycoheterotrophic plants are lacking. N concentrations in orchid tissue were unusually high and significantly higher than in accompanying autotrophic leaf samples. This may be caused by N gain of E. aphyllum from obligate ectomycorrhizal fungi. We show that E. aphyllum is an epiparasitic mycoheterotrophic orchid that depends on ectomycorrhizal Inocybe and Hebeloma to obtain C and N through a tripartite system linking mycoheterotrophic plants through fungi with forest trees.


PMID: 21309973

DOI: 10.1111/j.1438-8677.2010.00369.x

Other references

Denis, J.R., 1967: Complements et corrections au Vol. 8 du traite de P. P. Grasse. (Morphologie des Arthropodes) Heme partie. Heteropteres: thorax de Saldula. Travaux du Laboratoire Zool Stn aquic Grimaldi Dijon, 73: 1-24

Yelland, M.J.; Sweeting, K.R.; Lyftogt, J.A.; Ng, S.Kay.; Scuffham, P.A.; Evans, K.A., 2011: Prolotherapy injections and eccentric loading exercises for painful Achilles tendinosis: a randomised trial. To compare the effectiveness and cost-effectiveness of eccentric loading exercises (ELE) with prolotherapy injections used singly and in combination for painful Achilles tendinosis. A single-blinded randomised clinical trial. The primary outcome m...

Nakamura Y.; Ohmi G.; Kinoshita S., 1991: A study of accommodation and pupillary response in vdt workers. Using a computer-assisted infrared optometer with iriscorder, we measured accommodative function and pupillary response in 83 female subjects who worked with visual display terminals (VDT) (Age: 31 .apprx. 50 yrs). Of the 83, 32 complained of asth...

Samsinak, K., 1957: Uber die an den Lamellicornien lebenden Milben aus der Familie Tyroglyphidae (Acari). Acta Societatis Entomologicae Bohemiae, 54: 180-188

Bai, X.; Zhang, W.; Tang, X.; Xin, Y.; Xu, Y.; Sun, H.; Luo, X.; Pu, J.; Xu, J.; Xiong, Y.; Lu, S., 2016: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China. Shiga toxin-producing Escherichia coli (STEC) are an emerging group of zoonotic pathogens. Ruminants are the natural reservoir of STEC. In this study we determined the prevalence and characteristics of the STEC in plateau pika (Ochotona curzoniae)...

Green, A.R.; McLean, N.R.; Warren, E.A., 1984: Some uses of hexcelite as a splintage material in plastic and burns surgery. The clinical use of Hexcelite in plastic surgery and burns to construct strong, light-weight splints and to provide protective cover for skin grafts is described. Several examples are presented to illustrate its simplicity and adaptability.

Schildknecht, H.; Kunzelmann, P.; Krauss, D.; Kuhn, C., 1972: On the chemistry of the web part 1 arthropod protective substances. Naturwissenschaften 59(3): 98-99

Ayala-Martin, E., 1974: The rabbit section, complement of all farms. I. Explotacion agraria: 10 13-25

Cameron A.A.; Cliffer K.D.; Dougherty P.M.; Willis W.D.; Carlton S.M., 1991: Histochemical changes in the dorsal horn of the rat following experimental peripheral neuropathy. Society for Neuroscience Abstracts 17(1-2): 436

Barone, R.; Poirier, J., 1955: The uterine mucosa of the mare. The descriptions are based on the macro- and microscopic examinations of the genital tracts of 43 mares sent for slaughter, and include variations in appearance at different ages, at various stages of the oestrous cycle and at pregnancy. M. N.