geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines


, : Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines. Autophagy 4(6): 744-753

Burkholderia pseudomallei is the causative agent of melioidosis, a tropical infection of humans and other animals. The bacterium is an intracellular pathogen that can escape from endosomes into the host cytoplasm, where it replicates and infects adjacent cells. We investigated the role played by autophagy in the intracellular survival of B. pseudomallei in phagocytic and non-phagocytic cell lines. Autophagy was induced in response to B. pseudomallei invasion of murine macrophage (RAW 264.7) cells and a proportion of the bacteria co-localized with the autophagy effector protein LC3, a marker for autophagosome formation. Pharmacological stimulation of autophagy in RAW 264.7 and murine embryonic fibroblast (MEF) cell lines resulted in increased co-localization of B. pseudomallei with LC3 while basal levels of co-localization could be abrogated using inhibitors of the autophagic pathway. Furthermore, induction of autophagy decreased the intracellular survival of B. pseudomallei in these cell lines, but bacterial survival was not affected in MEF cell lines deficient in autophagy. Treatment of infected macrophages with chloramphenicol increased the proportion of bacteria within autophagosomes indicating that autophagic evasion is an active process relying on bacterial protein synthesis. Consistent with this hypothesis, we identified a B. pseudomallei type III secreted protein, BopA, which plays a role in mediating bacterial evasion of autophagy. We conclude that the autophagic pathway is a component of the innate defense system against invading B. pseudomallei, but which the bacteria can actively evade. However, when autophagy is pharmacologically induced using rapamycin, bacteria are actively sequestered in autophagosomes, ultimately decreasing their survival.

US$29.90

PMID: 18483470


Other references

Moshkova, L.V.; Skulkova, R.S.; Guseva, V.T., 1974: Use of statistical methods in evaluating the production activities of assistants in cost accounting pharmacies. Farmatsiia 23(3): 10-15

Chan, S.H.; Tan, T., 1989: HLA and allopurinol drug eruption. Strong HLA associations were found in Southern Chinese patients with skin eruptions due to allopurinol. The positive associations were with AW33 (Pc = 1.7 .times. 10-5; relative risk [RR] = 15.0, 95% confidence limit 5.2-42.9) with B17/BW58 (Pc =...

Phares, G.A.; Walent, J.H.; Niece, R.L.; Kumar, S.B.; Ericsson, L.H.; Kowalak, J.A.; Lloyd, P.E., 1996: Primary structure of a new neuropeptide, cerebral peptide 2, purified from cerebral ganglia of Aplysia. We report the purification and characterization of a novel neuropeptide from Aplysia nervous tissue. The peptide was termed cerebral peptide 2 (CP2) because it was the larger of two peptides predominantly synthesized in the cerebral ganglia and tr...

Doetsch, R.N.; Pelczar, M.J., 1948: The microbacteria; morphological and physiological characteristics. A representative collection of species of the genus Microbacterium Orla-Jensen was studied. Morphologically, the organisms were found to be of 3 distinct types; viz., short, thin rods characteristic of M. lacticum; rod-shaped cells 0.7-0.9 X 1.4-2...

Jagadeesan, J.; Oudit, D.; Hardwicke, J.; Shariff, Z.; McCoubrey, G.; Roberts, G.; Howcroft, A., 2006: Solitary plasmocytoma of frontal bone presenting as an asymptomatic forehead lump. Solitary plasmocytoma of bone is a rare type of plasma cell tumor. We present a case of a solitary extramedullary plasmacytoma of the frontal bone presenting as an asymptomatic forehead lump with clinically benign characteristics. This case highli...

Pohl, C.; Block, W.; Karitzky, J.; Träber, F.; Schmidt, S.; Grothe, C.; Lamerichs, R.; Schild, H.; Klockgether, T., 2001: Proton magnetic resonance spectroscopy of the motor cortex in 70 patients with amyotrophic lateral sclerosis. Objective: To evaluate proton magnetic resonance spectroscopy for detection and monitoring of upper motoneuron degeneration in patients with amyotrophic lateral sclerosis. Methods: Seventy patients with amyotrophic lateral sclerosis according to t...

Rodionov, V.V.; Shabarshov, I.A., 1975: Present beekeeping methods. This is a book on large-scale beekeeping with multi-storey hives in the USSR. The chapter on the utilization of nectar flows covers e.g. lime, buckwheat, sunflower, mixed flowers in Central Russia, the Volga and Higher Urals; buckwheat and heather...

Marini, M.A.; Casas, J.G.; Kien, M.C., 1989: Cutaneomucous lentiginosis and cutaneous and cardiac myxomas carney's complex. We report a 12 year old white boy with multiple cutaneous myxomas, acquired cutaneo-mucosal lentiginosis and cardiac myxoma. His father had died at the age of 34, of a thyroid carcinoma. This is an autosomal dominant inherited syndrome, previously...

Goldzveig, M.S.; Visconti, P.C.; Serrano, Z.R., 1958: Estimation of oestrogenic activity in subterranean clover. The oestrogenic activity of subterranean clover, Trifolium subterraneum, Dwalganup and Mount Barker varieties from 2 regions of Chile, was tested on immature female " lauchas " of 7.5 to 12 g. and ovariectomised virgin rats of 30 to 53 g...

Coombs, D.; Loyacano, A.; Joost, R., 1990: Stocker beef performance and stand persistence of low-endophyte tall fescue in central Louisiana. Proceedings of the Forage and Grassland Conference: 20-124