+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly

, : Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly. Nucleic Acids Research 42(21): 13430-9

Many ribosome-interacting GTPases, with proposed functions in ribosome biogenesis, are also implicated in the cellular regulatory coupling between ribosome assembly process and various growth control pathways. EngA is an essential GTPase in bacteria, and intriguingly, it contains two consecutive GTPase domains (GD), being one-of-a-kind among all known GTPases. EngA is required for the 50S subunit maturation. However, its molecular role remains elusive. Here, we present the structure of EngA bound to the 50S subunit. Our data show that EngA binds to the peptidyl transferase center (PTC) and induces dramatic conformational changes on the 50S subunit, which virtually returns the 50S subunit to a state similar to that of the late-stage 50S assembly intermediates. Very interestingly, our data show that the two GDs exhibit a pseudo-two-fold symmetry in the 50S-bound conformation. Our results indicate that EngA recognizes certain forms of the 50S assembly intermediates, and likely facilitates the conformational maturation of the PTC of the 23S rRNA in a direct manner. Furthermore, in a broad context, our data also suggest that EngA might be a sensor of the cellular GTP/GDP ratio, endowed with multiple conformational states, in response to fluctuations in cellular nucleotide pool, to facilitate and regulate ribosome assembly.


PMID: 25389271

DOI: 10.1093/nar/gku1135

Other references

Parrini, C.; Panconesi, A.; Govi, G.; Tunioli, F.; Raddi, P.; Intini, M., 1977: Coryneum disease of cypress (Cupressus sempervirens) in Tuscany. A series of 4 papers describing the prevention and control of the disease (caused by Coryneum cardinale) by cultural and chemical methods, the possibility of breeding for resistance, and the symptoms and pathology of the disease. Some tolerance wa...

Juncosa Morros, T.; Palacín Camacho, E.; Latorre Otín, C., 2005: Salmonellosis in a maternity-children's hospital in Barcelona over a 10-year period (1992-2001). We performed a retrospective study of Salmonella spp. strains, as well as of serotypes and resistance to antimicrobial agents that could be useful in salmonellosis requiring antibiotic treatment. All Salmonella strains isolated in a maternity-chil...

Cranston P.S.; Edward D.H.D.; Colless D.H., 1987: Archaeochlus brundin a midge out of time diptera chironomidae. Archaeochlus, a plesiomorphic genus of Podonominae (Chironomidae), is revised and two new species (biko from Namibia and brundini from southwestern Australia) described. The systematic status of the genus and the tribe Boreochlini is reassessed. B...

Wilson-Ashworth, H.A.; Bahm, Q.; Erickson, J.; Shinkle, A.; Vu, M.P.; Woodbury, D.; Bell, J.D., 2006: Differential detection of phospholipid fluidity, order, and spacing by fluorescence spectroscopy of bis-pyrene, prodan, nystatin, and merocyanine 540. The properties of liquid-ordered, solid-ordered, and liquid-disordered phases were investigated by steady-state fluorescence spectroscopy in liposomes composed of mixtures of dipalmitoylphosphatidylcholine and cholesterol (0-40 mol %) as a functio...

Kelch, R.P.; Hopwood, N.J.; Sauder, S.; Marshall, J.C., 1985: Evidence for decreased secretion of gonadotropin-releasing hormone in pubertal boys during short-term testosterone treatment. Information about the site(s) of action as well as the age-dependent effects of sex steroids on gonadotropin-releasing hormone and gonadotropin secretion during human puberty is limited. To begin to address these questions, we evaluated the effect...

Sandu Ville C.; Iacob V.; Hatmanu M.; Mititiuc M., 1967: Micromycetes new to science occurring in romania. Institutul Agronomic "Ion Ionescu De La Brad" Iasi Lucrari Stiintifice : 173-178

Gonzalez Tizon, A.; Martinez Lage, A.; Marinas, L.; Freire, R.; Cornudella, L.; Mendez, Y., 1998: Cytogenetic characterization of Donax trunculus. Cytogenetics & Cell Genetics 81(2): 109

Pesut, D.P.; Bulajic, M.V.; Nagomi-Obradovic, L.M.; Grgurevic, A.D.; Gledovic, Z.B.; Ponomarev, D.R.; Blanka, A.Z., 2012: Asthma mortality in Serbia: a 30-year analysis. The asthma mortality pattern differs among countries. No published evidence is currently available on asthma mortality in a European low-middle-income country in socioeconomic transition. We analyzed the trend of asthma mortality rate in Serbia du...

Sheft, I.; Fried, S., 1948: Quartz spring Jolly balance. Review of Scientific Instruments 19(10): 723-723

Rey, Jérôme.; Giustiniani, Jérôme.; Mallet, F.; Schiavon, Vèrie.; Boumsell, L.; Bensussan, A.; Olive, D.; Costello, Régis.T., 2006: The co-expression of 2B4 (CD244) and CD160 delineates a subpopulation of human CD8+ T cells with a potent CD160-mediated cytolytic effector function. Within human CD8(+) T lymphocytes, the CD27-CD45RA(high) or CD56(+) phenotypes contribute to precisely define the cells with CTL effector function. Novel markers were demonstrated to correlate with CTL properties, such as the 2B4 (CD244) receptor,...