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Structure, tissue distribution and estrogen regulation of splice variants of the sea bream estrogen receptor α gene


, : Structure, tissue distribution and estrogen regulation of splice variants of the sea bream estrogen receptor α gene. Gene 503(1): 18-24

Estrogen actions are mainly mediated by specific nuclear estrogen receptors (ERs), for which different genes and a diversity of transcript variants have been identified, mainly in mammals. In this study, we investigated the presence of ER splice variants in the teleost fish gilthead sea bream (Sparus auratus), by comparison with the genomic organization of the related species Takifugu rubripes. Two exon2-deleted ERα transcript variants were isolated from liver cDNA of estradiol-treated fish. The ΔE2 variant lacks ERα exon 2, generating a premature termination codon and a putative C-terminal truncated receptor, while the ΔE2,3* variant contains an in-frame deletion of exon 2 and part of exon 3 and codes for a putative ERα protein variant lacking most of the DNA-binding domain. Both variants were expressed at very low levels in several female and male sea bream tissues, and their expression was highly inducible in liver by estradiol-17β treatment with a strong positive correlation with the typical wild-type (wt) ERα response in this tissue. These findings identify novel estrogen responsive splice variants of fish ERα, and provide the basis for future studies to investigate possible modulation of wt-ER actions by splice variants.

US$19.90

PMID: 22579469

DOI: 10.1016/j.gene.2012.04.081


Other references

Waldbott, G.L., 2011: Does the routine treatment of asthma need revision?. Annals of Allergy 5(2): 126-131

Lennard-Jones, J.E., 1949: Measurement of swelling of an infected finger. Lancet 1(6550): 429-429

Oades, J.M.; Swinger, G.D., 1968: Effect of time of sampling and cropping sequences on the carbohydrates in red-brown earths. Flotation on a heavy liquid removed 2/5 of the total soil carbohydrate from partly decomposed plant remains. Carbohydrates were further extracted from the soils by 0.2 N NaOH which was more efficient with a soil cropped continuously with wheat tha...

Kozlova, I.A.; Frolova, M.I.; Kuznetsova, L.K., 1998: Antibiotic susceptibility of pneumonia pathogens in newborns and problems of antibacterial therapy of the pathological process. The results of the bacteriological investigation of the secretion from the trachea, large bronchi and fauces of 36 newborns (including 27 preterms) with severe pneumonia were analyzed. 20 of them were born of women with complicating somatic, obste...

Tsukada K.; Ito N., 1971: Poly nucleotide ligase from rat liver after partial hepatectomy and hepatoma. Proceedings of the Symposium on Chemical Physiology & Pathology 11: 36-40

Langworthy, O.R.; Ortega, L., 1943: Sensory endings on gastric muscle. Physiologists have long maintained that a variety of visceral sensations arise in the muscular coats of hollow organs. In the case of the stomach, sensations of hunger, distention and pain related to muscle cramp appear to rise in the muscular wal...

Owens, R.A.; Thompson, S.M.; Kramer, M., 2003: Identification of neutral mutants surrounding two naturally occurring variants of Potato spindle tuber viroid. Single point mutations in the pathogenicity domain of Potato spindle tuber viroid (PSTVd) can have a dramatic effect on disease expression, and only three substitutions are required for the spontaneous conversion of the type strain PSTVd-Intermedi...

Grover, S.; Rajeshwari, 2010: Global warming and its impact on skin disorders. Indian Journal of Dermatology, Venereology and Leprology 75(4): 337-339

Tonndorf, J.; Khanna, S.M., 1972: Tympanic-membrane vibrations in human cadaver ears studied by time-averaged holography. Journal of the Acoustical Society of America 52(4): 1221-1233

Marcinkiewicz S., 1972: Novel methods of preparation of 3 amino propan 1 ol derivatives part 2 synthesis of 3 n piperazinyl propan 1 ol and its derivatives. Acta Poloniae Pharmaceutica (english Translation): 20-25