+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications

, : Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. Journal of Biological Chemistry 288(39): 27801-27811

Osteocalcin (OC) and matrix Gla protein (MGP) are considered evolutionarily related because they share key structural features, although they have been described to exert different functions. In this work, we report the identification and characterization of both OC and MGP from the Adriatic sturgeon, a ray-finned fish characterized by a slow evolution and the retention of many ancestral features. Sturgeon MGP shows a primary structure, post-translation modifications, and patterns of mRNA/protein distribution and accumulation typical of known MGPs, and it contains seven possible Gla residues that would make the sturgeon protein the most γ-carboxylated among known MGPs. In contrast, sturgeon OC was found to present a hybrid structure. Indeed, although exhibiting protein domains typical of known OCs, it also contains structural features usually found in MGPs (e.g. a putative phosphorylated propeptide). Moreover, patterns of OC gene expression and protein accumulation overlap with those reported for MGP; OC was detected in bone cells and mineralized structures but also in soft and cartilaginous tissues. We propose that, in a context of a reduced rate of evolution, sturgeon OC has retained structural features of the ancestral protein that emerged millions of years ago from the duplication of an ancient MGP gene and may exhibit intermediate functional features.


PMID: 23884418

DOI: 10.1074/jbc.M113.450213

Other references

Kim, E.J.; Oh, J.H.; Cho, H.J.; Jung, S.J.; Kim, J.H.; Lee, H.J.; Shim, I.; Lee, H.S.; Lee, B.H., 2002: Inhibitory effects of acupuncture and electroacupuncture on the mechanosensitivity of articular afferents in arthritic rats. Acupuncture has traditionally been used in oriental medicine to relieve arthritic pain. In the present study, the effect of acupuncture and electroacupuncture on the activity of primary afferents was examined in an animal model of arthritic pain....

Bagel, O.; Degrand, C.; Limoges, B., 1999: Ion-exchange voltammetry as a solid-phase microextraction analytical method:  factors influencing the mass transfer to perfluorosulfonated ionomer film-coated electrodes and some of their consequences on the current responses. A simple theoretical model of mass transfer kinetics at an electrode coated with an ion-exchange film polymer is proposed. The model takes into account the analyte depletion in solution and gives the relationship between the ion-exchange voltammet...

Smith P.H., 1975: Electron microscopic evidence of a schwann cell modification in dog pancreatic islets. Anatomical Record 181(2): 484

Miranda, C.R.; Matsuoka, T., 2008: Nanogeoscience; theres plenty of room at the ground. Geochimica et Cosmochimica Acta 72.12S: A634

Hsu, C-Yi.; Su, Y-Wen.; Chen, Y-Tai.; Tsai, S-Hung.; Chang, C-Chin.; Li, S-Yuan.; Huang, P-Hsun.; Chen, J-Wen.; Lin, S-Jong., 2016: Association between use of oral-antidiabetic drugs and the risk of aortic aneurysm: a nested case-control analysis. Pleiotropic effects on cardiovascular protection have been suggested in several oral antidiabetic drugs (OAD). The impacts of OADs on aortic aneurysm (AA) growth have been found in animal studies, but the evidence of their beneficial effects for A...

Couet, C.; Fukagawa, N.K.; Matthews, D.E.; Bier, D.M.; Young, V.R., 1990: Plasma amino acid kinetics during acute states of glucagon deficiency and excess in healthy adults. The effects of glucagon deficiency and excess on plasma leucine, lysine, and alanine were examined in six healthy young adult men, with primed continuous infusions of L-[1-13C]- or L-[5,5,5-2H3]leucine, L-[.alpha.-15N]-lysine, and L-[3-13C]alanine...

Dupouy, V.M.; Ferre, P.J.; Uro-Coste, E.; Lefebvre, H.P., 2006: Time course of COX-1 and COX-2 expression during ischemia-reperfusion in rat skeletal muscle. Time course of COX-1 and COX-2 expression during ischemia-reperfusion in rat skeletal muscle. J Appl Physiol 100: 233-239, 2006; doi:10.1152/japplphysiol.00673.2004.- The aim of this study was to assess cyclooxygenase (COX)-1 and COX-2 expression...

Schijf Johan; de Barr Hein J.W., 1995: Rare earth element exchange through the Bosporus; the Black Sea as a net source of REEs to the Mediterranean Sea. The Bosporus is the only source of seawater to the Black Sea and helps to maintain the basin-wide salinity gradient that caused the Black Sea to become the largest permanently anoxic basin in the world, some 3000 years ago. Concentrations of disso...

Gahukar, Rt, 1975: New techniques adopted for the rearing of Ostrinia nubilalis Hubner (Lepidoptera, Pyraustidae) on artificial diet. Annales de zoologie: Ecologie animale: (4) 491-498

Stepanaytys N.Ye, 1979: The biostratigraphic significance of lower Tournaisian and Middle Visean ostracods of the western slope of the Central Urals. Voporsy Mikropaleontologii: ages 37-42. 1979.