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Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats


, : Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats. Experimental Animals 59(1): 35-45

WMN/Nrs inbred rats have been widely used in radiation biology for years. However, their reproductive profile has never been examined. We examined various reproductive characteristics of WMN/Nrs inbred rats such as superovulatory response, oocytes spontaneous activation (OSA), and embryo development in vitro and in vivo. Superovulation was induced in 3- to 9-week-old females by injection of 150 IU/kg PMSG and 150 IU/Kg hCG by 48 h apart. Only 8- and 9-week-old animals superovulated averaging 31.4 and 43.9 oocytes, respectively, and superovulation did not depend on estrous cycle. Animals 3-7 weeks of age did not superovulate. Because Wistar strains have been known to show a high incidence of OSA, factors expected to affect OSA in WMN/Nrs, including the time interval of various steps from euthanasia to oocyte recovery, incubation media, estrous cycle, and anesthetic treatments, were examined. The time from animal euthanasia to oviduct excision was the only factor shown to affect OSA. We also compared in vitro and in vivo embryo developmental competence between embryos obtained by natural ovulation and superovulation. Although percent in vitro development of 2-cell embryos to blastocysts was similar for embryos obtained by natural ovulation (63.7%) and superovulation (69.7%), fetus development after oviductal transfer of 2-cell embryos was significantly lower in embryos obtained by superovulation than in those obtained by natural ovulation (60.2% vs. 87.5%, P=0.02). Our results provide important normative data regarding future applications of rat assisted reproductive technologies (ARTs) such as in vitro fertilization and cryopreservation in WMN/Nrs strain and may be applicable to other strains of laboratory rats.

US$29.90

PMID: 20224168


Other references

Sandholt, I.; Poulsen, J.N.hom; Stjernholm, M.; Fog, B.; Skriver, H., 1995: Classification of agricultural crops in Denmark using ERS-1 SAR and SPOT Imagery. Askne, J [Editor] Sensors and environmental applications of remote sensing 37-44

Palmal, S.; Basiruddin, S.K.; Maity, A.Ranjan.; Ray, S.C.; Jana, N.R., 2013: Thiol-directed synthesis of highly fluorescent gold clusters and their conversion into stable imaging nanoprobes. Fluorescent gold clusters (FGCs) with tunable emission from blue to red and quantum yields in the range of 6-17% have been synthesized by simple modification of the conditions used for the synthesis of gold nanoparticles, namely by replacing the s...

Garofallo, S.Bueno.; Machado, D.Pinheiro.; Rodrigues, C.Garcia.; Bordim, O.; Kalil, R.A.K.; Portal, V.Lúcia., 2015: Applicability of two international risk scores in cardiac surgery in a reference center in Brazil. The applicability of international risk scores in heart surgery (HS) is not well defined in centers outside of North America and Europe. To evaluate the capacity of the Parsonnet Bernstein 2000 (BP) and EuroSCORE (ES) in predicting in-hospital mor...

Sridhar, T.; Ullasa, B.; Sohi, H., 1975: Occurrence of a new disease on grape seedlings caused by Phytophthora nicotianae var. parasitica (Dastur.) Waterhouse from India. Current science: 44 (11) 406

Playford, D.A.; Watts, G.F.; Best, J.D.; Burke, V., 2002: Effect of fenofibrate on brachial artery flow-mediated dilatation in type 2 diabetes mellitus. American Journal of Cardiology 90(11): 1254-1257

Gnibidenko Z.N., 1989: Magnetobiostratigraphic section and paleomagnetic characteristics of Pliocene deposits in the southwestern Siberian Plain. A magneto-biostratigraphic Pliocene sediment profile of the southern Siberian Lowland has been drawn on the basis of paleomagnetic studies on stratotypes of Novostanichnaya and Rytovskaya formations in Omsk Irtysh profiles which are compared to ea...

Romer, A.S., 1969: Cynodont reptile with incipient mammalian jaw articulation. A diagnostic mammalian character is jaw articulation between squamosal and dentary bones, replacing the quadrate-articular joint of reptiles. A newly discovered Argentinian Middle Triassic form shows, for the first time in an ancestral reptile, de...

Santini, V.; Scappini, B.; Gozzini, A.; Ferrini, P.R.ssi, 1997: Mechanism of Syk kinase activation after G-CSF stimulation of myeloid cells. Blood 90(10 SUPPL 1 PART 1): 311A, Nov 15

Anthony, A., 1999: Neutrophils in NSAID-induced injury. Alimentary Pharmacology & Therapeutics 13(8): 1131-1132

Goldstein, S.J.; Tibbs, P.A., 1981: Recurrent subarachnoid hemorrhage complicating cerebral arterial ectasia. Case report. A case of subarachnoid hemorrhage (SAH) complicating cerebral arterial ectasia is reported. While ischemia and cranial nerve palsies are commonly associated with this condition, review of the literature reveals that SAH is exceedingly rare. The pa...