geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Suppression of aberrant transient receptor potential cation channel, subfamily V, member 6 expression in hyperproliferative colonic crypts by dietary calcium


, : Suppression of aberrant transient receptor potential cation channel, subfamily V, member 6 expression in hyperproliferative colonic crypts by dietary calcium. American Journal of Physiology. Gastrointestinal and Liver Physiology 299(3): G593-G601

Dietary calcium is believed to reduce colon cancer risk, but the mechanism by which this occurs is poorly understood. Employing the Citrobacter rodentium-induced transmissible murine colonic hyperplasia (TMCH) model, we previously showed that a high-calcium diet (hCa) significantly abrogated hyperplasia in the distal colons of NIH-Swiss mice. Here, we explored the mechanism of dietary protection by hCa by analyzing the expression of genes involved in the regulation of Ca uptake/flux in the intestinal epithelium, including the Ca-sensing receptor, vitamin D receptor, Ca binding protein, and transient receptor potential cation channels, subfamily V, members 5 and 6 (TRPV5/6). Interestingly, while TRPV6 expression increased significantly during TMCH, the expression of the other gene products was unchanged. This elevated TRPV6 expression was significantly abrogated by a hCa diet. Immunofluorescence revealed apical membrane localization of TRPV6 in the normal colon, whereas during TMCH we observed intense apical pole and cytoplasmic staining along the entire longitudinal crypt axis, including the expanded proliferating zone. The hCa diet reversed this effect. In humans, overexpression of TRPV6 was associated with early-stage colon cancer, and in colon carcinoma cells, inhibition of TRPV6 expression by small interfering RNA inhibited their proliferation and induced apoptosis. TRPV6 small interfering RNA also diminished the transcriptional activity of the calcium-dependent nuclear factors in activated T cells. Thus the aberrant overexpression of TRPV6 contributes to colonic crypt hyperplasia in mice and to colon cancer cell proliferation in humans. Therefore, it is likely that suppression of TRPV6 by a hCa diet is required for its protective effects in the colon.

US$19.90

PMID: 20508153

DOI: 10.1152/ajpgi.00193.2010


Other references

Berrini, C.-Ceschi; De-Appolonia, F.; Dalla-Valle, L.; Komarek, J.; Andreoli, C., 2004: Morphological and molecular characterization of a thermophilic cyanobacterium (Oscillatoriales) from the Euganean Thermal Springs (Padua, Italy). The Euganean thermal mud is mostly colonized by cyanobacteria and microalgae which seem to be the responsible of the therapeutic properties on rheumatic diseases of mud therapy. The most abundant and widespread taxon among the cyanobacteria belong...

Ren, X.; Wang, W.; Zhang, X.; Pu, Y.; Wang, M.; Jiang, T., 2006: The comparative study of magnetic resonance angiography diagnosis and pathology of blunt vertebral artery injury. Study Design. Observational and comparative study of the vertebral artery injury detected by 2-dimensional (2-D) time-of-flight magnetic resonance angiography (MRA) for admitting patients. Construct a strike-induced flexion injury model of cervic...

Lerch, R.; Benzi, R., 1990: Physiopathological basis of protection of the ischemic myocardium. Ischemia initiates a cascade of intracellular events that culminates in the death of the myocyte. Reperfusion of ischemic myocardium is the most efficient way of salvaging jeopardized cells. Additional measures referred to as "myocardial prot...

Moriyama, M., 1989: Cellular architecture of papillary and nonpapillary transitional cell carcinoma. To characterize the cellular architecture of papillary and nonpapillary transitional cell carcinoma. 2 normal ureters, 6 papillary bladder cancers and 5 nonpapillary bladder cancers were subjected to light and electron microscopic study as well as...

Anonymous, 2011: A model for predicting resuspension ofEscherichia colifrom streambed sediments. To improve the modeling of water quality in watersheds, a model is developed to predict resuspension ofE. colifrom sediment beds in streams. The resuspension rate is expressed as the product of the concentration ofE. coliattached to sediment parti...

Sobieraj, D.M.; White, C.Michael.; Parker, M.; Bookhart, B.K.; Coleman, C.I., 2016: Quality measures for anticoagulation care. To systematically identify and assess current quality measures for anticoagulation in atrial fibrillation (AF), venous thromboembolism (VTE) treatment and surgical VTE prophylaxis. Fifteen quality measure websites were searched as were bibliograph...

Rayburn, J.M.; Rayburn, L.G., 1994: Marketing implications of the shift in power of the hospital. With the introduction of the Prospective Payment System, hospital accountants' role changed from reimbursement maximizers to an important role in decision making. Faced with increased competition, hospitals are installing financial controls....

Magne, J.; Pibarot, P.; Trahan, S.; Sénéchal, M., 2008: Dysfunction of a bileaflet mechanical valve in mitral position: absence of symptoms despite a completely fixed leaflet. We report the case of 62 year-old asymptomatic woman with a bileaflet mechanical prosthesis implanted 1 year ago. Routine transthoracic echocardiography (TTE) showed that one leaflet was fixed in semi-closed position, which was confirmed by transe...

Hanisch, E.; Beyer, P.; Heller, K.; Paolucci, V.; Jung, M.; Schaeff, B., 1994: Potentially dangerous moments in use of esophageal balloon tamponade. 4 case reports with critical analysis of the literature. In general, balloon tamponade is still part of the therapy of bleeding esophageal varices, despite effective sclerotherapy. Four case reports demonstrate that the application of balloon tamponade may be associated with severe complications. Moreov...

Zakhari, R.B., 1964: Role Of The Time Factor In Emergency Surgery On Newborn Infants. Khirurgiia 40: 8-10