+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Suppression of aggressive strains of 'Candidatus phytoplasma mali' by mild strains in Catharanthus roseus and Nicotiana occidentalis and indication of similar action in apple trees

, : Suppression of aggressive strains of 'Candidatus phytoplasma mali' by mild strains in Catharanthus roseus and Nicotiana occidentalis and indication of similar action in apple trees. Phytopathology 104(5): 453-461

To study antagonistic interactions of 'Candidatus Phytoplasma mali' strains, graft inoculation of Catharanthus roseus and Nicotiana occidentalis was performed with mild strains 1/93Vin and 1/93Tab as suppressors and three aggressive strains as challengers. Inoculation of the suppressors was carried out in either the cross-protection modus prior to grafting of the challengers or by co-inoculating suppressors and challengers. Monitoring using multiplex real-time polymerase chain reaction assays revealed that, in long-term cross-protection trials with C. roseus, suppressor 1/93Vin was present in all root and randomly collected stem samples over the entire observation period. In contrast, the challengers were never detected in such stem samples and rarely in the roots. Following simultaneous inoculation, the suppressor successively colonized all stem and root regions whereas detection of challenger AT steadily decreased. However, this strain remained detectable in up to 13 and 27% of stem and root samples, respectively. The cross-protection trials with N. occidentalis yielded results similar to that of the cross-protection experiments with C. roseus. Comparison of the symptomatology of infected apple trees with the presence of putatively suppressive strains indicated that suppression of severe strains also occurs in apple. Phylogenetic analysis using a variable fragment of AAA+ ATPase gene AP460 of 'Ca. P. mali' revealed that suppressors 1/93Vin and 1/93Tab, together with several other mild strains maintained in apple, cluster distantly from obviously nonsuppressive strains that were predominantly highly virulent.


PMID: 24724815

DOI: 10.1094/PHYTO-08-13-0230-R

Other references

Platt, L.; Thun, M.; Harriman, K.; Winter, K., 2017: A Population-Based Study of Recurrent Symptomatic Bordetella pertussis Infections in Children in California, 2010-2015. Natural infection with Bordetella pertussis is thought to result in 4-20 years of immunity against subsequent symptomatic pertussis infection. However, these estimates are based on studies in unvaccinated or whole-cell pertussis-vaccinated childre...

Rosenblum, S.; Waissman, P.; Diamond, G.W., 2016: Identifying play characteristics of pre-school children with developmental coordination disorder via parental questionnaires. NlmCategory="UNASSIGNED">Motor coordination deficits that characterize children with Developmental Coordination Disorder (DCD) affect their quality of participation. The aim of the current study was to identify play characteristics of...

Gupta, V.; Nath, S.; Chand, S., 2002: Electrostatic interactions, phase separation behaviour and partitioning of proteins in polyelectrolyte based aqueous two-phase systems. Electrostatic interactions play a major role in the purification of proteins by different methods and they tend to be specific in nature in the presence of salts and environmental hydrogen ion concentration (pH). These can be readily exploited as...

Navdaev, A.; Clemetson, K.J., 2002: Glycoprotein Ib cross-linking/ligation on echicetin-coated surfaces or echicetin-IgMkappa in stirred suspension activates platelets by cytoskeleton modulated calcium release. Cross-linking platelet GPIb with the snake C-type lectin echicetin provides a specific technique for activation via this receptor. This allows GPIb-dependent mechanisms to be studied without the necessity for shear stress-induced binding of von Wi...

Siripoonya, P.; Tejavej, A., 1980: Congenital abnormalities in the early neonatal period: ten years incidence at Ramathibodi Hospital. Journal of the Medical Association of Thailand 63(10): 544-547

Pezzilli, R.; Morselli Labate, A.M.; Casteliano, E.; Barbera, C.; Corrao, S.; D.P.ima, L.; Lucidi, V.; Carroccio, A., 2001: Acute pancreatitis in children An Italian multicenter study. Gastroenterology 120(5 Supplement 1): A 471, April

Evans, P.C.; Smith, T.S.; Lai, M-Jiun.; Williams, M.G.; Burke, D.F.; Heyninck, K.; Kreike, M.M.; Beyaert, R.; Blundell, T.L.; Kilshaw, P.J., 2003: A novel type of deubiquitinating enzyme. A previous report from this laboratory described two novel proteins that have sequence similarity to A20, a negative regulator of NF-kappaB (Evans, P. C., Taylor, E. R., Coadwell, J., Heyninck, K., Beyaert, R., and Kilshaw, P. J. (2001) Biochem. J...

Mitsui, T.K.sezawa, N.G.da, T., 2007: Milk consumption does not affect body mass index but may have an unfavorable effect on serum total cholesterol in Japanese adults. Milk consumption is considered to have favorable effects on obesity, blood pressure, and cholesterol. However, studies conducted on Japanese populations are sparse, and a positive association between milk intake and increased level of serum total...

Polteva, Y.K., 1964: Some urgent problems in pediatric nutrition. Research is needed in the requirements of breast-fed infants for vitamins, particularly vitamin D, and in the feeding of children aged over 1 year. Most investigations of diets of older children is on their requirements for protein, to its quantit...

Hall, R.J.; Martin, C.C.; Hardesty, B.E., 1989: Advances in electronic reproduction of the clinical bedside examination for cardiovascular education. Texas Heart Institute Journal 16(1): 1-4