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TNFRp55 modulates IL-6 and nitric oxide responses following Yersinia lipopolysaccharide stimulation in peritoneal macrophages

, : TNFRp55 modulates IL-6 and nitric oxide responses following Yersinia lipopolysaccharide stimulation in peritoneal macrophages. Immunobiology 216(12): 1322-1330

While cytokines are major regulators of macrophage activation following host-pathogen interactions, they also act to limit inflammation to avoid tissue damage. In previous studies we reported the development of progressive Yersinia enterocolitica-induced reactive arthritis (ReA) in mice lacking the tumor necrosis factor receptor p55 (TNFRp55). In this work, we analyzed the response of TNFRp55⁻/⁻ macrophages to Y. enterocolitica antigens. We found higher concentration of nitric oxide (NO) in TNFRp55⁻/⁻ compared to wild-type macrophages in response to heat-killed Yersinia (HKY) and Yersinia outer membranes (OM). Moreover, Toll-like receptor (TLR)4 expression was increased in OM-stimulated TNFRp55⁻/⁻ versus wild-type (WT) macrophages. Accordingly, NO production was inhibited in TLR4-deficient macrophages following stimulation with OM, suggesting that LPS may function as a major OM component implicated in these responses. Thus, augmented NO production together with enhanced expression of inducible nitric oxide synthase (iNOS) and higher IL-6 production, may provide a pro-inflammatory setting in Yersinia LPS-stimulated TNFRp55⁻/⁻ macrophages. Augmented synthesis of NO and IL-6 was prevented by treatment with Polymyxin B, or by exposure to a specific NF-κB p65 oligonucleotide antisense, indicating the involvement of TLR4-mediated NF-κB activation in the unleashed pro-inflammatory response triggered by TNFRp55 deficiency. Thus, TNFRp55 modulates macrophage functions in response to Yersinia LPS stimulation through mechanisms involving NO, IL-6 and NF-κB pathways, suggesting an essential regulatory role of TNF via TNFRp55 signaling.


PMID: 21802165

DOI: 10.1016/j.imbio.2011.05.009

Other references

Rundquist, P.J.; Ludewig, P.M., 2005: Correlation of 3-dimensional shoulder kinematics to function in subjects with idiopathic loss of shoulder range of motion. People with idiopathic loss of shoulder range of motion (ROM) have difficulty completing activities of daily living. This investigation was performed to determine the association between active glenohumeral ROM and function and to develop a multip...

Sahni G.; Acharya A.S., 1989: Semisynthetic approach for site specific mutations in the alpha chain of hemoglobin s. Whitten, C F And J F Bertles (Ed ) Annals Of The New York Academy Of Sciences, Vol 565 Sickle Cell Disease; Conference, Bethesda, Maryland, Usa, April 11-13, 1988 Xiv+477p New York Academy Of Sciences: New York, New York, Usa Illus Maps 458-459

Hoffmann, M.; Lang, M.; Sommer, J-Uwe., 2011: Gelation threshold of cross-linked polymer brushes. The cross-linking of polymer brushes is studied using the bond-fluctuation model. By mapping the cross-linking process into a two-dimensional (2D) percolation problem within the lattice of grafting points, we investigate the gelation transition in...

Shi, X.Yang.; Dumenyo, C.Korsi.; Hernandez-Martinez, R.; Azad, H.; Cooksey, D.A., 2007: Characterization of regulatory pathways in Xylella fastidiosa: genes and phenotypes controlled by algU. Many virulence genes in plant bacterial pathogens are coordinately regulated by "global" regulatory genes. Conducting DNA microarray analysis of bacterial mutants of such genes, compared with the wild type, can help to refine the list of...

Biniecki, S.; Herold, F., 1970: Synthesis of N,N'-bis(beta-phenylisopropyl)-1,3-diaminopropane. Acta Poloniae Pharmaceutica 27(6): 529-532

Anonymous, 1979: Utility and futility. Talanta 26(2): I-i

Abstracts., 2002: Abstracts of the 1st Polish-Czech-German Conference, Jelenia Gora 6th July 2002 Bats of the Sudety Mts. Auritus, 12: 1-7

Anella, L.B.; Schnelle, M.A.; Maronek, D.M., 2000: Oklahoma Proven A statewide marketing and evaluation program. Hortscience 35(4): 555, July

Haditsch J.G.; Yamac Y., 1977: Review of mapping of the middle and upper Sarmatian deposits near Straden in eastern Styria. Mitteilungen der Abteilung fuer Geologie, Palaeontologie und Bergbau am Landesmuseum Joanneum: ages 77-78. 1977.

Kovachevski, I., 1976: New host plants of the lucerne mosaic virus in Bulgaria. Of 24 hosts of alfalfa mosaic virus listed, 18 are new.