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Tear fluid proteome variability in healthy donors


, : Tear fluid proteome variability in healthy donors. Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 99(4): 527-536

The variability of control samples is the main problem in clinical proteomics and reliable sources for estimation of variability of normal content of different proteins are not numerous. Most of the investigations of normal human tear proteome include pool of samples as reference materials. But it is impossible to use such experimental approach to establish the range of variability of different tear proteome components. This study is the first attempt to estimate the variability of proteins content in healthy donors tear using high-performance liquid chromatography and tandem mass spectrometry. The protein profiles from 11 individual healthy donors were analyzed. Essential variability of the minor proteins was revealed while the presence of 6 major proteins in all 11 samples was invariable. We found the Lacritin glycosylation in all samples of tear fluid received from healthy donors. It seems that this modification is typical for healthy donors tear. The analysis of the pool samples was also carried out to estimate the availability for this approach for the search of ophthalmologic diseases biomarkers.

US$29.90

PMID: 23862393


Other references

Vermeiren, H.; Willems, A.; Schoofs, G.; de Mot, R.; Keijers, V.; Hai, W.; Vanderleyden, J., 1999: The rice inoculant strain Alcaligenes faecalis A15 is a nitrogen-fixing Pseudomonas stutzeri. The taxonomic position of the nitrogen-fixing rice isolate A15, previously classified as Alcaligenes faecalis, was reinvestigated. On the basis of its small subunit ribosomal RNA (16S rRNA) sequence this strain identifies as Pseudomonas stutzeri....

Geoghegan, K.F.; Dallas, J.L.; Feeney, R.E., 1980: Periodate inactivation of ovotransferrin and human serum transferrin. Azari and Phillips (Azari, P., and Phillips, J. L. 1970 Arch. Biochem. Biophys. 138, 32-38) reported that periodate treatment of iron-free ovotransferrin causes a rapid loss of iron-binding activity and an oxidation of 3 to 5 tyrosines and 1 trypt...

Bohrer, K., 1987: Larval food and caste differentiation in Lasioglossum malachurum. The following parameters of L. malachurum larval food were examined: content and composition of free fatty acids (FFA), proteins, free amino acids and the content of reducing sugars. Emphasis was given to the FFA analyses. On the basis of behaviou...

Timmermans, K.; van der Wal, S.Ei.; Vaneker, M.; van der Laak, J.Awm.; Netea, M.G.; Pickkers, P.; Scheffer, G.Jan.; Joosten, L.Ab.; Kox, M., 2013: IL-1β processing in mechanical ventilation-induced inflammation is dependent on neutrophil factors rather than caspase-1. Mechanical ventilation can cause ventilator-induced lung injury, characterized by a sterile inflammatory response in the lungs resulting in tissue damage and respiratory failure. The cytokine interleukin-1β (IL-1β) is thought to play an importan...

Agarwal R.; Jugert F.K.; Merk H.F.; Bickers D.R.; Mukhtar H., 1992: Induction of cytochrome p 450 isozymes 1a 2b and 3a in murine skin by pyridine. Journal of Investigative Dermatology 98(4): 652

White, P.C., 1977: A survey of medical care in the United States Merchant Marine. Military Medicine 142(8): 613-616

Gilka, W.; Paasivirta, L., 2009: Evaluation of diagnostic characters of the Tanytarsus chinyensis group Diptera Chironomidae, with description of a new species from Lapland. Tanytarsus salmelai sp. n. collected from the northernmost sites of the Finnish Lapland is described. The new species and two sibling Tanytarsus, T. brundini Lindeberg, 1963 and T. curticornis Kieffer, 1911, are compared, diagnosed and illustrated...

Anonymous, 2012: Association of ErbB1–4 expression in invasive breast cancer with clinicopathological characteristics and prognosis. Human epidermal growth factor receptor type 2 (Her2)/ErbB2 plays a key role in the initiation and progression of invasive breast cancer. However, the prognostic relevance to breast cancer patients of the other ErbB family members has long been a m...

Miettinen, O.S.; Henschke, C.I.; Yankelevitz, D.F., 1999: Evaluation of diagnostic imaging tests: diagnostic probability estimation. In the evaluation of a diagnostic imaging test for the diagnosis of a particular illness in a particular category of patients, the test should be construed as leading to a test result in the sense of a set of descriptive readings from the image(s)...

Huppert, J.D.; Pasupuleti, R.V.; Foa, E.B.; Mathews, A., 2007: Interpretation biases in social anxiety: response generation, response selection, and self-appraisals. Cognitive theories propose that the resolution of ambiguity is related to the maintenance of social anxiety. A sentence completion task was used to examine how individuals high (n = 26) and low (n = 23) in social anxiety resolve ambiguous social...