geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions


, : The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. Development 139(24): 4582-4590

Establishment and maintenance of epigenetic memories are essential for development. Replacement of canonical histone H3 by its variant H3.3 has been implicated in cellular memory. Drosophila sequence-specific DNA-binding protein GAGA factor and a chromatin factor FACT direct H3.3 replacement in conjunction with H3.3-specific chaperone HIRA at chromatin boundaries to counteract the spreading of silent chromatin. However, little is known about which ATP-driven chromatin remodeling factor is responsible for the H3.3 replacement at chromatin boundaries. Here, we report that GAGA factor associates with the Polybromo-associated Brm (PBAP) remodeling complex, which consists of many Trithorax group proteins, and recruits this complex to chromatin boundaries d1 (which is downstream of w), the Fab-7 DNase-hypersensitive site (HS) 1 of Abd-B and the bxd region of Ubx. Trl-encoding GAGA factor, brm and polybromo/bap180 mutations compromise the H3.3 replacement and boundary functions in a synergistic manner. Furthermore, Polybromo is necessary for generation of the DNase HS at d1, and HIRA functions to restore the alteration. Taken together, we propose that FACT and PBAP complexes are recruited to chromatin boundaries in a GAGA factor-dependent manner, and are needed for H3.3 replacement to execute boundary functions. Our results provide new insight into the function of the trithorax group during development.

US$19.90

PMID: 23136390

DOI: 10.1242/dev.083246


Other references

Kawaguchi T.; Nagashima R.; Noda M.; Shioya A., 1973: Mechanisms of protection by cps 161 ethyl d gluco fururonoside p ethoxyphenyl hydrazide against carbon tetra chloride induced liver injury. Japanese Journal of Physiology 23(SUPPL): 26

Goodyer, P.R.; Kachra, Z.; Bell, C.; Rozen, R., 1988: Renal tubular cells are potential targets for epidermal growth factor. Epidermal growth factor (EGF) is a potent polypeptide mitogen with various receptor-mediated growth effects on cells from the skin, breast, and gastrointestinal tract. Recent studies indicate that EGF is produced in the kidney and is excreted in t...

Deflaun, M.F.; Streger, S.H.; Gillespie, K.M.; Dong, H.; Onstott, T.C.; Fuller, M.E., 1999: Investigation of bacterial transport by intact core experiments. Abstracts of the General Meeting of the American Society for Microbiology 99: 418

Fowler, J.F.; Lawrey, J.M., 1960: Dose-rate effects on mice and rats in the range 2 to 340 rads/minute. The effect of 230 kV X-rays at 2 different intensities was compared in 3 biological systems: (1) skin and hair reactions of mouse legs were compared after single doses of about 2000 rads given at 340 or 65 rads/minute; (2) the percentage mortality...

Berger, H.; Hilbertz, T.; Zühlke, K.; Forst, H.; Pratschke, E., 1993: Balloon dilatation and stent placement of suprahepatic caval anastomotic stenosis following liver transplantation. We present an unusual case of suprahepatic caval anastomotic stenosis after adult liver transplantation in the early postoperative period. Color flow Doppler sonography and cavography were used to confirm the clinical diagnosis. Successful nonoper...

Keimer; Casa; Ivanov; Lynn; Zimmermann; Hill; Gibbs; Taguchi; Tokura, 2000: Spin dynamics and orbital state in LaTiO3. A neutron scattering study of the Mott-Hubbard insulator LaTiO3 ( T(N) = 132 K) reveals a spin wave spectrum that is well described by a nearest-neighbor superexchange constant J = 15.5 meV and a small Dzyaloshinskii-Moriya interaction ( D = 1.1 m...

Zhao-Hai-Zhen; Zhang-Zhi-Xiang; Liao-Me-De; Zhang-Jing-Li; Xu-Han-Hong, 2006: Insecticidal activities of propiconazole against Spodoptera litura (Lepidoptera : Noctuidae). On the ground of studying the cytotoxicity of 11 kinds of pesticides, propiconazole, which had the highest toxicity against Spodoptera litura (SL) cells, was chosen to further investigate its toxicology. The results showed that the mortality of St...

Zhang, T.; Qi, Y.; Liao, M.; Xu, M.; Bower, K.A.; Frank, J.A.; Shen, H-Ming.; Luo, J.; Shi, X.; Chen, G., 2013: Autophagy is a cell self-protective mechanism against arsenic-induced cell transformation. Subchronic exposure to arsenic increases the incidence of human cancers such as skin, lung, colon, and rectal cancer. The mechanism for arsenic-induced tumorigenesis is still not clear. It is generally believed that DNA damage and genomic instabil...

Giambelluca, T.W., 2002: Hydrology of altered tropical forest. The influences of land cover change on hydrologic processes, especially the effects of clearing forest, are a major environmental concern and the subject of much scientific research. In recognition of the rapid pace of deforestation in the Amazon...

Heintz, C.E.; Pramer, D., 1972: Ultrastructure of nematode-trapping fungi. The ultrastructure is described of the nematode-trapping cells of Dactylella drechslerii (adhesive knobs), Monacrosporium rutgeriensis (adhesive network) and Arthrobotrys dactyloides. There are 4 plates of electronmicrographs.