geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The Pneumocystis Ace2 transcription factor regulates cell wall-remodeling genes and organism virulence


, : The Pneumocystis Ace2 transcription factor regulates cell wall-remodeling genes and organism virulence. Journal of Biological Chemistry 288(33): 23893-23902

Pneumocystis carinii (Pc) β-glucans are major components of the organism cell wall; yet, the regulation of Pc cell wall genesis and remodeling is not well understood. Ace2 transcription factors, which are present in many fungi, regulate glucanases and other enzymes needed for cell wall remodeling. The cloning and heterologous expression of PcAce2 in ace2Δ Saccharomyces cerevisiae demonstrated that PcAce2 can restore the defective glucanase and endochitinase gene expression of the mutant as well as regulate cell wall β-glucan biosynthetic genes. Furthermore, when a reconstructed yeast system was used, PcAce2 activated the transcription of the Pneumocystis gsc1 β-glucan synthetase, confirming the activity of a Pc transcription factor on a native Pneumocystis promoter and gene for the first time. We further observed that Pneumocystis binding to host extracellular matrix proteins and lung epithelial cells induced the phosphorylation (activation) of the PcAce2 transcription factor. Finally, we present a novel method that confirms the role of PcAce2 in modulating organism virulence using ace2Δ Candida glabrata infection in neutropenic mice. Together, these results indicate that the adherence of Pc to lung matrix proteins and epithelial cells leads to the activation of the Ace2 transcription factor, which regulates cell wall degradation and biosynthesis genes that are required for cell wall remodeling.

US$19.90

PMID: 23801335

DOI: 10.1074/jbc.M113.471243


Other references

Edwards C.C.; Levine A.M., 1987: Posterior reduction and fixation for unstable l4 s1 injuries. Orthopaedic Transactions 11(1): 19-20

Ashraf, M.; Yasmin, H., 1991: Differential waterlogging tolerance in three grasses of contrasting habitats aeluropus lagopoides l. trin. cynodon dactylon l. pers. and leptochloa fusca l. kunth. High concentrations of reduced iron and manganese, developed as a result of anoxia in waterlogged soils, cause considerable growth inhibition in many plant species. In order to determine the effect of waterlogging on three grass species, Aeluropus...

Axt, J.C.; Mccaffery, J.M., 1993: Reduction of postoperative against-the-rule astigmatism by lateral incision technique. A group of 80 preoperative against-the-rule (AR) eyes had cataract removal and intraocular lens implantation with lateral (temporal) incision and suturing. Fifty-six of the 80 eyes had extracapsular cataract extraction (ECCE) and 24 had phacoemuls...

Topouzis, F.; Coleman, A.L.; Jonescu Cuypers, C.; Yu, F.; Harris, A.; Mavroudis, L.; Anastasopoulos, E.; Kagemann, L.; Koskosas, A.; Wilson, M.R., 2003: Systemic factors affecting optic disc topographic heidelberg retina tomograph parameters in the general population. Purpose: To identify factors associated with optic disc topographic HRT parameters in a random sample of the general population. Methods: In 94% of 263 consecutive subjects (248 subjects) over 60 years of age in the Thessaloniki Eye Study, we obta...

Gage, A.J.; Sommerfelt, A.E.; Piani, A.L., 1997: Household structure and childhood immunization in Niger and Nigeria. In this study, we use data from the Demographic and Health Surveys to examine the relationship between household structure and childhood immunization in Niger and Nigeria. We show that household structure is an important determinant of childhood i...

Carlson R.H.; Bridges C.V.; Williams P.S., 1982: Effects of health locus of control and health value upon electro myography bio feedback training. Subjects (human) who scored highly internal or external on a health locus of control scale and who scored high or low on the value each attached to good health were each given 2 sessions of frontalis EMG (electromyographic) biofeedback training in...

Chieruzzi, M.; Rallini, M.; Pagano, S.; Eramo, S.; D'Errico, P.; Torre, L.; Kenny, Jé.M., 2014: Mechanical effect of static loading on endodontically treated teeth restored with fiber-reinforced posts. The aim of this study was to investigate the mechanical behavior of a dental system built up with fiber-reinforced composite (FRC) endodontic posts with different types of fibers and two cements (the first one used with a primer, the second one wi...

Ishimoto, S., 1971: The eyes of nursing. Connecting mind with mind. Japanese Journal of Nursing 23(3): 1-2

Bharani, A.; Kumar, H., 2001: Diabetes inspidus induced by ofloxacin. BMJ 323(7312): 547

Gascon, J., 2003: Infectious diseases and immigration. This article discusses infectious diseases and immigration, in Europe and Spain in particular, including common infections in immigrants, such as tuberculosis, HIV infection and parasitic infections (neurocysticercosis and strongyloidiasis). Impor...