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The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers

, : The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers. Tissue Engineering. Part A 16(9): 2731-2741

Interactions between hepatocytes and liver sinusoidal endothelial cells (LSECs) are essential for the development and maintenance of hepatic phenotypic functions. We report the assembly of three-dimensional liver sinusoidal mimics comprised of primary rat hepatocytes, LSECs, and an intermediate chitosan-hyaluronic acid polyelectrolyte multilayer (PEM). The height of the PEMs ranged from 30 to 55 nm and exhibited a shear modulus of approximately 100 kPa. Hepatocyte-PEM cellular constructs exhibited stable urea and albumin production over a 7-day period, and these values were either higher or similar to cells cultured in a collagen sandwich. This is of significance because the thickness of a collagen gel is approximately 1000-fold higher than the height of the chitosan-hyaluronic acid PEM. In the hepatocyte-PEM-LSEC liver-mimetic cellular constructs, LSEC phenotype was maintained, and these cultures exhibited stable urea and albumin production. CYP1A1/2 activity measured over a 7-day period was significantly higher in the hepatocyte-PEM-LSEC constructs than in collagen sandwich cultures. A 16-fold increase in CYP1A1/2 activity was observed for hepatocyte-PEM-10,000 LSEC samples, thereby suggesting that interactions between hepatocytes and LSECs are critical in enhancing the detoxification capability in hepatic cultures in vitro.


PMID: 20491586

DOI: 10.1089/ten.tea.2009.0695

Other references

Drain, P.K.; Rousseau, C., 2017: Point-of-care diagnostics: extending the laboratory network to reach the last mile. More point-of-care (POC) diagnostic tests are becoming available for HIV diagnosis and treatment in resource-limited settings. These novel technologies have the potential to foster decentralized HIV care and treatment for the benefit of clinical l...

Lintilhac, J.P.; Tichadou, M., 1966: The umbilicus in plastic surgery of the abdomen. Transposition or reconstruction. Maroc Medical 45(490): 266-271

Knueppel, R.C.; Rahimian, J., 2007: Diffuse cutaneous hyperpigmentation due to tigecycline or polymyxin B. Clinical Infectious Diseases 45(1): 136-138

Kosztyła-Hojna, Bżena.; Moskal, D.; Falkowski, D.; Łobaczuk-Sitnik, A.; Kraszewska, A.; Skorupa, Młgorzata., 2016: Rehabilitation of phonosurgically treated patients with edematous-hypertrophic changes of larynx. Advanced change of organic dysphonia are an indication for phonosurgery. Edematous-hypertrophic changes are cause of serious disturbances of voice. High-speed digital imaging (HSDI) technique is the unique method, allowing for assessment the effec...

Kehrberg, C., 1988: Pay equity--what it means and how it affects nurses. Kansas Nurse 63(10): 6-6

Cai, Y.; Gu, H.; Kenney, T., 2017: Learning Microbial Community Structures with Supervised and Unsupervised Non-negative Matrix Factorization. Learning the structure of microbial communities is critical in understanding the different community structures and functions of microbes in distinct individuals. We view microbial communities as consisting of many subcommunities which are formed...

Hou, J.; Cliver, S.; Tamura, T.; Goldenberg, R., 1999: Maternal serum ferritin concentration in relation to small for gestational age infants. American Journal of Obstetrics & Gynecology 180(1 PART 2): S179, Jan

Southgate, J.; Varley, C.L.; Garthwaite, M.A.E.; Hinley, J.; Marsh, F.; Stahlschmidt, J.; Trejdosiewicz, L.K.; Eardley, I., 2007: Differentiation potential of urothelium from patients with benign bladder dysfunction. To develop a novel in vitro approach to test the hypothesis that failure of urothelial differentiation underlies the aetiopathology of interstitial cystitis (IC), where there is evidence of compromised urinary barrier function, as benign dysfunct...

Mrazek, A., 1916: Cestoden-Studien. II. Die morphologische Bodeutung der Cestoden-Larven. Zoologische Jahrbuecher Jena Abteilungen fur Anatomie, 39 515-584

Dinse H., 1981: Effects of velocity direction and intensity on hyper complex cells in the cat area 18. Neuroscience Letters. 7: S147