+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The differential response to Fgf signalling in cells internalized at different times influences lineage segregation in preimplantation mouse embryos

, : The differential response to Fgf signalling in cells internalized at different times influences lineage segregation in preimplantation mouse embryos. Open Biology 3(11): 130104-130104

Lineage specification in the preimplantation mouse embryo is a regulative process. Thus, it has been difficult to ascertain whether segregation of the inner-cell-mass (ICM) into precursors of the pluripotent epiblast (EPI) and the differentiating primitive endoderm (PE) is random or influenced by developmental history. Here, our results lead to a unifying model for cell fate specification in which the time of internalization and the relative contribution of ICM cells generated by two waves of asymmetric divisions influence cell fate. We show that cells generated in the second wave express higher levels of Fgfr2 than those generated in the first, leading to ICM cells with varying Fgfr2 expression. To test whether such heterogeneity is enough to bias cell fate, we upregulate Fgfr2 and show it directs cells towards PE. Our results suggest that the strength of this bias is influenced by the number of cells generated in the first wave and, mostly likely, by the level of Fgf signalling in the ICM. Differences in the developmental potential of eight-cell- and 16-cell-stage outside blastomeres placed in the inside of chimaeric embryos further support this conclusion. These results unite previous findings demonstrating the importance of developmental history and Fgf signalling in determining cell fate.


PMID: 24258274

DOI: 10.1098/rsob.130104

Other references

Karatas, O.F.; Guzel, E.; Duz, M.B.; Ittmann, M.; Ozen, M., 2016: The role of ATP-binding cassette transporter genes in the progression of prostate cancer. Prostate cancer (PCa) is the most commonly diagnosed neoplasm and the second leading cause of cancer-related death among men in developed countries. There is no clear evidence showing the success of current screening tests in reducing mortality of...

Simones, T.; Shepherd, D.M., 2011: Consequences of AhR activation in steady-state dendritic cells. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the prototypical aryl hydrocarbon receptor (AhR) ligand and a potent immunotoxicant. However, the mechanisms underlying TCDD-induced immunomodulation remain to be defined. Dendritic cells are professio...

Vaidya, R.; Siragusa, S.; Huang, J.; Schwager, S.M.; Hanson, C.A.; Hussein, K.; Pardanani, A.; Tefferi, A., 2010: Mature survival data for 176 patients younger than 60 years with primary myelofibrosis diagnosed between 1976 and 2005: evidence for survival gains in recent years. In the past 20 years, management of primary myelofibrosis (PMF) has incorporated new treatment approaches, but survival benefits have not been confirmed in controlled studies. This retrospective study includes 176 consecutive patients younger than...

Plata-Colin, A., 1982: Report of Forest Technological Institute No. 1, El Salto, P.N., Durango. Publicacion especial Instituto Nacional de Investigaciones Forestales: (36) 43-51

Ackerman, N.W., 1970: What happened to the family?. Mental Hygiene 54(3): 459-463

Rasmussen, R.A.; Morgal, P.W.; Miller, E.J., 1943: Composition of fat from a North American black bear. Fat from the subcutaneous adipose tissues of a $ black bear (Ursus americanus), from Ontario, contained 71.7% oleic and 17.4% linoleic acid glycerides. Tabulated data show the physical and chemical properties of bear fat as published by other inve...

Hall, R.H.B., 1995: Sheep breeds, wool preparation, wool classing and management practices in the Falkland Islands during 1990/91. A survey by questionnaire of sheep producers on the Falkland Islands showed that Corriedales accounted for 61% of the sheep population, the remainder being Polwarths (27%) and Romneys (12%). The questionnaire showed that 69% of producers were plan...

Kostis J.B.; Wilson A.C.; Tannenbaum A.K.; Pan H.Y.; Kuo P.T., 1989: Long term combination therapy of primary hypercholesterolemia with pravastatin. Circulation 80(4 SUPPL 2): II128

McClintock, D.W., 1982: Politics and economics of genetic diversity. The technical and institutional challenges, the financial problems, the political ramifications and the need for improvements in international communication are considered in relation to the conservation of world genetic resources.

Jeon, J.; Sato, K.; Niedzwiecki, D.; Ye, X.; Saltz, L.B.; Mayer, R.J.; Mowat, R.B.; Whittom, R.; Hantel, A.; Benson, A.; Wigler, D.S.; Atienza, D.; Messino, M.; Kindler, H.; Venook, A.; Fuchs, C.S.; Meyerhardt, J.A., 2014: Impact of physical activity after cancer diagnosis on survival in patients with recurrent colon cancer: Findings from CALGB 89803/Alliance. The impact of physical activity on survival outcomes in patients with recurrent colon cancer has not been studied. We tested the association between the level of postdiagnosis physical activity and survival outcomes of patients with recurrent colo...